牙槽骨牙周膜来源干细胞的鉴定

qianqianlaile

原文来源于pubmed，由干细胞之家新闻小组成员 qianqianlaile 翻译（转帖请注明）
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组织工程A部 2010-12-27 付印前电子版
3 F9 ^& i3 \! T牙槽骨牙周膜来源干细胞的鉴定
( `" p6 y( D. R8 [0 _中华人民共和国 西安 第四军医大学  口腔医学院 正畸科
) [4 b  R/ `# ?# |5 z摘要
' v- I/ J) `/ J; o: r# s( L复合的牙周结构即牙骨质、牙周膜和牙槽骨的完整而predicable再生对牙周研究者来说是一个很大的调战。一般认为人牙根表面的牙周膜中含有能增强体内类牙骨质/牙周膜结构再生的干细胞(r-PDLSCs)。本研究用长效溴脱氧尿苷 (BrdU)标记物观察发现牙周膜拥有不对称分布的干细胞。分离并鉴定人牙槽骨表面的牙周膜假定的干细胞(a-PDLSCs)。结果显示a-PDLSCs 有强的增殖能力并高比例表达间充质干细胞标记物。成骨和成脂分化时，a-PDLSCs的多向分化潜力较r-PDLSCs的高，而且a-PDLSCs的碱性磷酸酶活性和矿化相关标记物的表达也高于r-PDLSCs。体内a-PDLSCs 能再生骨/类牙周膜结构并修复临界尺寸的NOD/SCID鼠颅骨上建模形成的缺损。自体PDLSC介导的牙周再生显示 a-PDLSCs重建的牙槽骨较r-PDLSCs重建的完美。结果表明不同的条件下的PDLSCs可能有完全不同的特性。a-PDLSCs 和r-PDLSCs在牙周膜再生中可能有协同作用。
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Tissue Eng Part A. 2010 Dec 27. [Epub ahead of print]% H7 D* U: G( H
Characterization of Stem Cells from Alveolar Periodontal Ligament.  I3 n0 K! J% j7 }- |( d
Wang L, Shen H, Zheng W, Tang L, Yang Z, Gao Y, Yang Q, Wang C, Duan Y, Jin Y.
7 F/ ^4 m) l3 N1 Department of Orthodontics, School of Stomatology, the Fourth Military Medical University , Xi'an, Shaanxi, People's Republic of China .7 K; c4 E$ L8 R
Abstract
2 M) t! s+ q- G* LComplete and predicable regeneration of complex periodontal structures, which include cementum, periodontal ligament (PDL), and alveolar bone, has been a great challenge for periodontal researchers. It is generally believed that human PDL from the root surface contains stem cells (r-PDLSCs), which can enhance cementum/PDL-like tissues regeneration in vivo. In this work, PDL was found to possess asymmetrically distributed stem cells observed by long-term bromodeoxyuridine (BrdU) labeling. Putative stem cells from human PDL on the alveolar bone surface (a-PDLSCs) were then isolated and characterized. It was shown that a-PDLSCs exhibited strong proliferation capability and expressed high percentages of mesenchymal stem cell markers. Comparatively, a-PDLSCs had higher multilineage differentiation potential than r-PDLSCs with regard to both osteogenic and adipogenic differentiation. Alkaline phosphatase activity and the expression of mineralization-related markers of a-PDLSCs were also higher than those of r-PDLSCs. In vivo, a-PDLSCs could regenerate bone/PDL-like structures and repair critical-size defects created in calvarial bone of NOD/SCID mice. Autologous PDLSC-mediated periodontal regeneration showed that a-PDLSCs could accomplish reconstruction of alveolar bone more perfectly than r-PDLSCs. Our data suggest that PDLSCs may have quite different characteristics depending on locations. a-PDLSCs may take a synergistic effect with r-PDLSCs in periodontal regeneration.3 z4 N/ O; D. d) S+ S7 \
PMID: 21186958 [PubMed - as supplied by publisher]( z: d7 i5 ]) Q1 g/ K4 [$ t* f
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注明：个人翻译，理解不准确的语句还望大家积极指出。
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