Oct4 Expression Is Not Required for Mouse Somatic Stem Cell Self-Renewal

fwyou

Summary8 W6 G+ o, S& P
The Pou domain containing transcription factor Oct4 is a well-established regulator of pluripotency in the inner cell mass of the mammalian blastocyst as well as in embryonic stem cells. While it has been shown that the Oct4 gene is inactivated through a series of epigenetic modifications following implantation, recent studies have detected Oct4 activity in a variety of somatic stem cells and tumor cells. Based on these observations it has been suggested that Oct4 may also function in maintaining self-renewal of somatic stem cells and, in addition, may promote tumor formation. We employed a genetic approach to determine whether Oct4 is important for maintaining pluripotency in the stem cell compartments of several somatic tissues, including the intestinal epithelium, bone marrow (hematopoietic and mesenchymal lineages), hair follicle, brain, and liver. Oct4 gene ablation in these tissues revealed no abnormalities in homeostasis or regenerative capacity. We conclude that Oct4 is dispensable for both self-renewal and maintenance of somatic stem cells in the adult mammal.% L! E; o0 V" x8 e
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huangcong1988

原文，之前有相关报道，不过是在SCR过程中，其作用可以被E-钙粘蛋白替代

huangcong1988

E-钙粘蛋白

qizhi502

E-cadherin是CAM的组成之一,重要的细胞与细胞之间的adhesin,胚胎过程是影响致密化,在小鼠ES中有高表达,具体是通过怎样的信号通路参与细胞内的代谢?也许只是合适的niche表现.本文只是证明了它存在的重要性(ES,reprogramming).

细胞海洋

回复 fwyou 的帖子# j; I: D3 ]" J

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新疆八钢

正常上皮细胞富于细胞间黏附从而形成稳定的细胞间接触。恶性肿瘤细胞上皮组织基本结构消失，失去细胞极性和紧密连接，细胞内骨架成分重排并获得移行能力等间质细胞的特性。恶性肿瘤细胞的迁徙和运动能力增强，摆脱细胞之间的连接，从而突破基底膜、侵袭周围组织。E-cadherin是上皮组织中的一种依赖Ca2+的细胞间跨膜粘连糖蛋白分子，主要参与细胞间的连接，E-cadherin同时也是上皮表型标志物。恶性肿瘤细胞具体是通过怎样的信号通路，调节细胞的代谢，下调E-cadherin的表达，获得迁徙和运动能力，有待遇深入研究。

always_wp

这个应该是原文吧7 t0 j/ h* z# c  }