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本帖最后由 grape 于 2011-7-19 10:12 编辑 5 _ r2 Q2 ~. ]
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4 j/ ^+ }5 I8 j/ G关于乙酰化抗体的选择 h( D7 W" N4 m# B% a" m
c5 u) v' ]+ g3 }# Shttp://www.immunechem.com/所提供的乙酰化抗体在世界的知名度很高，其抗乙酰化抗体发表的文章有近20多篇，大部份发表在Cell, Science, Nature, Molecular Cell等一流杂志，主要条带集中在组蛋白、p53和能量代谢的线粒体蛋白；Dr. chunaram choudhary采用他们的抗体，分别在2009, 2010发表在Science两篇高引用率的文献；Haigis, Leonard采用他们的抗体，在2009发表在Cell.$ v( k3 W& S! L# H; G( `/ c9 [
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Acetyl Lysine Antibody" o5 c$ ]$ o- |! b* Z6 m
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CatalogNumber Pack Size Price(CNY)
- c# U4 s) o L9 O4 d2 l+ QICP0380 100 µg ￥2,000.00
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; n. }4 ]$ z) DProduct Description: This pan-specific antibody is affinity purified using acetyllysine affinity chromatography. It recognizes proteins with acetyllysine residues. The product has been utilized for proteomic studies of the protein acetylation, immunoaffinity charomatography separation and isolation of the acetylated protein and peptides from protease-digesting proteins of the whole cells.
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% I+ @8 C9 {6 s2 Z) D. b) PSpecies: Rabbit
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Formulation: 250 µg/mL antibody stored in PBS, 50% glycerol
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Storage & Stability: Store product at -20 ℃. Three years
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from date of shipping.
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Immunogen: Acetylated KLH conjugates
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Purification: The antibody was purified on acetyl-lysine agarose
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Specificity: The antibody recognizes proteins acetylated on lysine residues. * @' t( B, A% p! m [$ B
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Application: ELISA,WB,IP,IHC,IF. % q5 F- J# o7 Z$ G1 |. U
. K+ X# [/ v. L# O* W( }Tested: Acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins
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Scientific Description: DNA transcription can not take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylation of lysine residues within the proteins. It has been suggested that acetylation of non-histones proteins (e.g., transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains.7 _' p, p$ r3 R& }8 \
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References: & l2 U0 I5 g" E$ M8 c
1. Zeng, L. et al FEBS Lett. 2002, 513, 124-128 (2002) . O* z6 Q$ B& n
2. Kim. S. et al Molecular Cell, Volume 23, Issue 4, Pages 607-618 9 }# F1 s K9 Q6 m7 `% R6 E6 M
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发表于 2011-7-19 09:14
发表于 2011-7-19 11:18
发表于 2012-7-1 15:19
