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年轻的齐藤通纪,在生殖细胞研究上近几年成果不断,差不多是这个领域最耀眼的年轻猩猩
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0 ^# E- n2 Z/ U/ R, m5 bCell. 2011 Aug 3. [Epub ahead of print]
: n( T' i1 h* K+ \0 b" CReconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells.0 |) I5 _. p1 P7 B+ B
Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M. Y3 ^! v8 O m) K- S* V4 T c
SourceDepartment of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; JST, CREST, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
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Abstract( ^% y& _) @3 t! |7 b
The generation of properly functioning gametes in vitro requires reconstitution of the multistepped pathway of germ cell development. We demonstrate here the generation of primordial germ cell-like cells (PGCLCs) in mice with robust capacity for spermatogenesis. PGCLCs were generated from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) through epiblast-like cells (EpiLCs), a cellular state highly similar to pregastrulating epiblasts but distinct from epiblast stem cells (EpiSCs). Reflecting epiblast development, EpiLC induction from ESCs/iPSCs is a progressive process, and EpiLCs highly competent for the PGC fate are a transient entity. The global transcription profiles, epigenetic reprogramming, and cellular dynamics during PGCLC induction from EpiLCs meticulously capture those associated with PGC specification from the epiblasts. Furthermore, we identify Integrin-β3 and SSEA1 as markers that allow the isolation of PGCLCs with spermatogenic capacity from tumorigenic undifferentiated cells. Our findings provide a paradigm for the first step of in vitro gametogenesis. m; E: _. v. l/ D, f2 ?& K
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