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本帖最后由 饶冠华 于 2011-8-27 14:56 编辑
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Stem Cells. 2010 Apr;28(4):649-60.4 U+ G# p$ c- o& o' X
Complex display of putative tumor stem cell markers in the NCI60 tumor cell line panel.! Z3 D9 C) z1 `( J. N4 r
Stuelten CH, Mertins SD, Busch JI, Gowens M, Scudiero DA, Burkett MW, Hite KM, Alley M, Hollingshead M, Shoemaker RH, Niederhuber JE.
; h0 y; l8 E( L% l! H/ E9 R4 u; ASourceCell and Cancer Biology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA. chrisstu@mail.nih.gov
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Abstract
' L5 ^, o3 w* ^7 y# m2 a' OTumor stem cells or cancer initiating cells (CICs) are single tumor cells that can regenerate a tumor or a metastasis. The identification and isolation of CICs remain challenging, and a variety of putative CIC markers have been described. We hypothesized that cell lines of the NCI60 panel contain CICs and express putative CIC markers. We investigated expression of putative CIC surface markers (CD15, CD24, CD44, CD133, CD166, CD326, PgP) and the activity of aldehyde dehydrogenase in the NCI60 panel singly and in combination by six-color fluorescence-activated cell sorting analysis. All investigated markers were expressed in cell lines of the NCI60 panel. Expression levels of individual markers varied widely across the 60 cell lines, and neither single marker expression nor simple combinations nor co-expression patterns correlated with the colony-formation capacity of cell lines. Rather, marker expression patterns correlated with tumor types in multidimensional analysis. Whereas some expression patterns correlated with tumor entities such as basal breast cancer, other expression patterns occurred across different tumor types and largely related to expression of a more mesenchymal phenotype in individual breast, lung, renal, and melanoma cell lines. Our data for the first time demonstrate that tumor cell lines display CIC markers in a complex pattern that relates to the tumor type. The complexity and tumor type specificity of marker display creates challenges for the application of cell sorting and other approaches to isolation of putative tumor stem cell populations and suggests that therapeutic targeting strategies will need to take this into account.& |/ B' B# K7 v5 q! {
# n# W9 Z3 _* q* x& CPMID: 20178109 [PubMed - indexed for MEDLINE] Free full text+ {1 m$ b: @0 x6 K5 y8 M' S
3 ~: U; u( w% \% X# r8 S0 _推荐理由：鉴于有很多人用细胞系来研究肿瘤干细胞相关的性质，但用细胞系所遇到的第一个问题就是，面对众说纷纭的肿瘤干细胞Markers，我们所使用的细胞应该使用哪些marker的组合呢？我们知道，怎样使用正确的肿瘤干细胞标志组合，这是任何基于肿瘤干细胞研究的基础问题。
. P; R2 s2 i+ v/ ^$ v: e本篇文章系统地研究了近60种肿瘤细胞系，并且囊括了绝大部分肿瘤干细胞研究所用到的markers。快来找找，看看有没有你需要的数据吧！ |
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发表于 2011-8-27 14:56
发表于 2011-8-27 19:02
