|
  
- 积分
- 6084
- 威望
- 6084
- 包包
- 18316
|
回复 naturalkillerce 的帖子& |3 j: T9 n9 j5 y/ I# v7 X5 ]
+ X" `! f% |3 rResearchers Grow Human Colon Stem Cells In Vitro6 U/ p$ u! J! k1 O, \
Sunday, September 04, 2011 - Stem Cell Research News
h6 X0 |& d' B, z" z" z9 U$ ?; z+ q: j; `* D: s" r
Spanish researchers have identified and grown human colon stem cells in vitro for the first time.) \/ o/ Q0 M- p9 O- n# [3 Q+ t! I
7 M6 L( G3 G* p" Q. [% xThroughout life, stem cells of the colon regenerate the inner layer of the human large intestine weekly.
. ?: r8 G* `$ k6 I; q1 [
* B4 H0 c8 q8 BFor decades scientists had evidence of the existence of these cells yet their identity remained elusive.% R' L8 C/ _" X( @9 R
* ^/ S; P3 t/ d0 o6 C! dScientists led by the ICREA Prof. Eduard Batlle, a researcher at the Institute for Research in Biomedicine (IRB Barcelona), discovered the precise localization of the stem cells in the human colon and worked out a method that allows their isolation and in vitro expansion in Petri dishes.
$ b) ?5 E* Z' f9 T" F$ e' H6 X# }$ l% b* ?1 y4 G, L0 ^
Growing cells outside the body generally requires providing the cells in a dish with the right mix of nutrients, growth factors and hormones.
0 K5 l- ? w7 d5 P' n8 L* h, }7 [; L9 v2 ^ y; U A% \
But in the same way that each of the more than 200 types of cells in our body differs from the others, so do optimal growing conditions in the lab.# k: t# x+ N/ M8 F
7 t* F' j& H( o- j% ]/ `/ M/ Y1 {* Z
Consequently, human adult stem cell culture in labs has been a truly impossible mission until now.( b4 n; o t/ F9 q
( m7 i, G+ c5 j, G
Batlle’s team also established the conditions for maintain living human colon stem cells (CoSCs) outside of the human body.
+ i9 d3 e8 K) N5 ^6 I5 e1 f* a ^9 Y. _9 N1 M( F B
“This is the first time that it has been possible to grow single CoSCs in lab-plates and to derive human intestinal stem cell lines in defined conditions in a lab setting,” said Peter Jung, first author of the study together with Toshiro Sato, from the University Medical Center Utrecht in The Netherlands.
5 \6 c1 X% M! [ L- ?: {1 }% Z" G7 z. t& J: ?/ R6 Q
The development occurred after more than ten years of research focused on the characterization of the biology of the intestinal stem cells and its connection with cancer.
" e, L& R0 O1 I/ X. @8 H0 P# M: Z( G# m+ D
“For years, scientists all over the world have been trying to grow intestinal tissue in lab-plates; testing different conditions; using different nutritive media. But because the vast majority of cells in this tissue are in a differentiated state in which they do not proliferate, they survived only for a few days,” Jung said. “The aim of this study was to find a way to identify and select individual CoSCs and to grow them while maintaining their undifferentiated and proliferative state in lab conditions. Thus, we would be able to model how they grow in number and differentiate into normal intestinal epithelial cells in lab-plates.
0 W8 I+ y/ H) V
! u) C8 ]$ y) r, g: Q7 Y- ~“The scientific community now has a defined ‘recipe’ for isolating CoSCs and deriving stable CoSCs lines, which have the capacity to grow undifferentiated for months. In fact, now we can maintain stem cells in a plate up to five months or we can induce these cells to differentiate artificially, as they do inside our bodies.”+ q" v }2 ~2 w" m( X/ c
* o9 T; ~5 m4 z7 S2 h$ ~. P3 z1 k
Jung said the achievement opens up an exciting new area of research with the potential to bring about a huge breakthrough in regenerative medicine.& L( X4 h& ^" y% Q
5 N' d3 n# Q! p- y: L( Z
Now that guidelines for growing and maintaining colon stem cells in the lab are in place, Jung said, there is an ideal platform that could help the scientific community to determine the molecular bases of gastrointestinal cell proliferation and differentiation. He suspects that alterations in the biology of CoSCs are at the origin of several diseases affecting the gastrointestinal tract, such as colorectal cancer and Crohn’s disease, an autoimmune and inflammatory disorder.
; F: s8 o6 _# V4 V3 c9 k* A9 E; C7 D" S( Y1 z6 F5 Y; q
“Our discovery paves the way to start exploring this exciting field,” Jung said.
6 f, }9 f6 H, v7 t0 b% k' }6 f$ Y, j5 x9 [3 S- Y7 x* B
The research was published on September 4 in Nature Medicine. |
|
发表于 2011-9-13 10:37
发表于 2011-9-13 13:48
