求助：肝细胞的流式分离

wanglibin

最近要用流式的方法来分离小鼠的成体肝细胞，请问能用哪些抗体来进行？请大家帮帮忙

naturalkillerce

回复 wanglibin 的帖子9 w  e3 R' \, |) a) M

6 K/ M4 D2 K* n4 s/ v$ [Isolated mouse hepatocyte purity check was introduced to monitor the expression of CD95 on hepatocytes using flow cytometry methods.5 I" d" b: B! [" R+ g. E, W$ W

, N. ?) ]/ X! ^& R" B  b因此，CD95抗体应该是需要的。

naturalkillerce

回复 wanglibin 的帖子
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给你提供一篇文献《Improved isolation of murine hepatocytes for in vitro malaria liver stage studies》供参考：% t/ L; e, ?, V# }
摘要为：
4 R7 G4 e- A& Z+ Q3 \This protocol is a detailed description of a technique to isolate and culture mouse/ k# b2 b8 N! y& U) S9 c1 C
hepatocytes and represents an improvement over previous descriptions of hepatocyte isolation for: ?: e) B+ n$ w+ e0 h
malaria studies, regarding three technical aspects: (1) dissociation reagents choice; (2) cell
( P/ f# @% a7 @+ o. e) kseparation gradient and (3) cell purity control. Cell dissociation was optimized for a specific
( F& |! T* b! [collagenase digestion media. The cell dissociation step was improved by using a three-layer# N  |! N/ t9 h4 b3 |- O
discontinuous gradient. A cell purity check was introduced to monitor the expression of CD95 on
- g& ]5 G+ c6 n& H/ x9 khepatocytes using flow cytometry methods.
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3 K" d1 }+ A" X( q2 x0 K; T 1475-2875-6-169.pdf (632.31 KB)

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wanglibin

回复 naturalkillerce 的帖子+ [5 H& A6 ?0 W2 N: u& \5 ]

* X3 y  W1 e! F% f谢谢你了~