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Modulation of mouse neural crest cell motility by N-cadherin and conne

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发表于 2009-3-5 23:31 |显示全部帖子
1 Biology Department, Goddard Laboratory, University of Pennsylvania, Philadelphia, PA 19104" v  c- |9 j! u+ E

1 r; n' W5 l/ E' G5 A- b( E2 Center for Research on Reproduction and Women's Health, School of Medicine of the University of Pennsylvania, Philadelphia, PA 19104
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Address correspondence to C.W. Lo, Biology Dept., Goddard Laboratory, University of Pennsylvania, Philadelphia, PA 19104-6017. Tel.: (215) 898-3693/8394. Fax: (215) 898-8780. E-mail:clo@sas.upenn.edu: k' r/ f. l; A# `
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Abstract9 \0 c7 ~! F' j; D) D' F

5 m; |- _% i4 aConnexin 43 (Cx431) gap junction has been shown to have an essential role in mediating functional coupling of neural crest cells and in modulating neural crest cell migration. Here, we showed that N-cadherin and wnt1 are required for efficient dye coupling but not for the expression of Cx431 gap junctions in neural crest cells. Cell motility was found to be altered in the N-cadherin–deficient neural crest cells, but the alterations were different from that elicited by Cx431 deficiency. In contrast, wnt1-deficient neural crest cells showed no discernible change in cell motility. These observations suggest that dye coupling may not be a good measure of gap junction communication relevant to motility. Alternatively, Cx431 may serve a novel function in motility. We observed that p120 catenin (p120ctn), an Armadillo protein known to modulate cell motility, is colocalized not only with N-cadherin but also with Cx431. Moreover, the subcellular distribution of p120ctn was altered with N-cadherin or Cx431 deficiency. Based on these findings, we propose a model in which Cx431 and N-cadherin may modulate neural crest cell motility by engaging in a dynamic cross-talk with the cell's locomotory apparatus through p120ctn signaling.
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Key Words: cadherin; connexin; wnt; neural crest; catenin3 z, d/ T4 |; n) U
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Introduction& [+ ]) o% q* J' r
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The deployment of migratory cells plays an important role in embryonic development and disease. In vertebrates, a remarkable migratory cell population known as the neural crest cells plays an essential role in many aspects of development. These cells are derived from the dorsal neuroepithelium by an epithelial–mesenchymal cell transformation. They migrate long distances along distinct pathways to many different regions of the embryo and contribute to the differentiation and development of a diverse array of tissues. Various developmental defects can arise when neural crest cells do not migrate along their stereotypical pathways, or for other reasons, failed to reach their intended target sites. For example, perturbation in the deployment of the cardiac neural crest cells can result in persistent truncus arteriosus and various aortic arch anomalies (Kirby and Waldo, 1995). Thus, elucidating the mechanisms regulating neural crest cell migration is of great interest not only given its importance in vertebrate development, but also such studies have significant clinical relevance.- W* w; z( m* y( Z, W
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Recent studies have indicated an essential role for gap junctions in modulating the migratory behavior of neural crest cells. Gap junctions contain membrane channels that allow the movement of ions, second messengers, and small metabolites between cells (for review see Bruzzone et al., 1996). They are encoded by a multigene family known as the connexins. The gap junction protein, connexin 43 or 1 connexin (Cx431)* is expressed abundantly in migrating neural crest cells, and these cells are also functionally coupled via gap junction channels (Lo et al., 1999). These observations are consistent with the fact that neural crest cells migrate not as individual cells but as cohorts of cells that are organized in sheets or streams (Bancroft and Bellairs, 1976; Davis and Trinkaus, 1981). When the Cx431 gene is deleted in the Cx431 knockout mice, conotruncal heart malformations and outflow obstruction were elicited (Reaume et al., 1995). Using transgenic mouse models, we showed that the requirement for Cx431 in conotruncal heart development involves the cardiac neural crest cells (Ewart et al., 1997; Huang et al., 1998a; Sullivan et al., 1998). Cardiac neural crest cells, a neural crest subpopulation derived from the postotic hindbrain neural fold, play an essential role in outflow tract septation and tissue remodeling in the conotruncal region of the heart. Transgenic mice exhibiting an elevation or inhibition of Cx431 function targeted to the cardiac neural crest cells show outflow tract obstruction and conotruncal heart malformations (Ewart et al., 1997; Huang et al., 1998b; Sullivan et al., 1998). These heart malformations are likely due to alterations in the abundance of neural crest cells reaching the heart. Thus an elevation of gap junction communication was associated with an enhanced rate of neural crest cell migration and an increase in the abundance of neural crest cells in the outflow tract (Huang et al., 1998a). In contrast, a reduction or inhibition of gap junction communication was associated with a reduced rate of neural crest cell migration, and concomitantly, a decrease in the abundance of crest cells in the outflow tract (Huang et al., 1998a). Based on these observations, we proposed previously that gap junctions may mediate the cell-to-cell movement of second messengers and other cell-signaling molecules involved in regulating cell locomotion and in this manner help coordinate the deployment of neural crest cells to the heart (Huang et al., 1998a; Lo and Wessels, 1998).
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4 V2 a/ @3 V  H& H+ |3 o! X; L( V0 IThe present study was initiated to examine the role of cadherin-based adherens junction in modulating gap junction communication and the migration of mouse cardiac neural crest cells. Studies in tissue culture cells have shown previously that gap junction formation is dependent on cadherin-mediated cell–cell adhesion (Keane et al., 1988; Mege et al., 1988; Matsuzaki et al., 1990; Musil et al., 1990; Jongen et al., 1991; Meyer et al., 1992; Frenzel and Johnson, 1996). In chick embryos, N-cadherin is expressed in the neural tube and emerging neural crest cells (Hatta and Takeichi, 1986). A specific requirement for N-cadherin in neural crest cell migration was shown by the finding that the inhibition of N-cadherin with function-blocking antibody perturbed the deployment of neural crest cells (Bronner-Fraser et al., 1992). In such antibody-treated embryos, neural crest cells were found inside the lumen of the neural tube or in the extraembryonic space dorsal to the neural tube but not in the expected dorsolateral neural crest migratory pathway. In addition, studies using adenoviral vectors to ectopically express various N-cadherin constructs have shown that overexpression of N-cadherin can disrupt the normal deployment of neural crest cells (Nakagawa and Takeichi, 1998). In the present study, we sought to elucidate the role of N-cadherin in the deployment of mouse cardiac neural crest cells and whether this involves the modulation of Cx431-mediated gap junction communication." j7 U- D. b0 @, w

$ d1 P: r! N8 p8 F2 \3 lOur studies show that N-cadherin has an essential role in mouse neural cell migration. Mouse neural crest cells express N-cadherin in regions of cell–cell contact found between extended cell processes. N-cadherin is often colocalized or closely juxtaposed with Cx431 gap junctions. Interestingly, in N-cadherin–deficient neural crest cells, although Cx431 gap junction contacts remained abundant, gap junction communication was reduced to levels like that of the Cx431 knockout mice. Motion analysis revealed that the speed and directionality of neural crest cell locomotion are affected differently by N-cadherin versus Cx431 deficiencies. These results suggest that N-cadherin and Cx431 gap junctions may have separable roles in neural crest cell locomotion. As ?-catenin expression was reduced in the N-cadherin but not Cx431-deficient neural crest cells, we further examined the possible involvement of wnt signaling in neural crest cell motility. Neural crest cells from the wnt1 knockout mouse embryos exhibited normal cell motility, even though gap junction communication was reduced to levels like that of Cx431-deficient neural crest cells. As with the N-cadherin–deficient neural crest cells, Cx431 gap junction contacts remained abundant at the cell surface in wnt1-deficient neural crest cells. These observations suggest that N-cadherin and wnt1 may regulate gating of Cx431 gap junction channels in neural crest cells. As cadherin is known to bind p120 catenin (p120ctn), an Armadillo protein also involved in modulating cell motility (Anastasiadis and Reynolds, 2000; Grosheva et al., 2000; Noren et al., 2000), we examined p120ctn expression in neural crest cells. p120ctn was observed to be expressed abundantly in neural crest cells. It was found to be colocalized with N-cadherin and Cx431. Significantly, the distribution of p120ctn was altered in the Cx431- and N-cadherin–deficient neural crest cells. Based on these findings, we propose that neural crest cell motility may be modulated by the dynamic interactions of N-cadherin and Cx431 with the cell's locomotory apparatus through p120ctn signaling.
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6 I' D! A3 H& U* z4 C% }Cx431 gap junctions in N-cadherin–deficient neural crest cells
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Our studies focused on the cardiac neural crest cells, a subpopulation of neural crest cells that emerge from the postotic hindbrain neuroepithelium at E8.5 (Fukiishi and Morriss-Kay, 1992). This is before the time when N-cadherin–deficient embryos die from cell adhesion defects in the developing myocardium (Radice et al., 1997). To determine if N-cadherin is expressed in the cardiac neural crest cells, postotic hindbrain neural tube explant cultures were generated, and immunofluorescence microscopy was used to examine the newly emerged neural crest cells. Such studies showed the distribution of N-cadherin diffusely over the cell surface and also clustered in punctate spots along extended cell processes, many of which were thin projections barely visible by phase–contrast microscopy (Fig. 1 A). This pattern is similar to that reported for N-cadherin expression in neural crest cells in chick neural tube explant cultures (Monier-Gavelle and Duband, 1995). Double immunostaining with Cx431 and N-cadherin antibodies revealed in some regions the close juxtapositioning or colocalization of N-cadherin with Cx431, particularly along cell processes (Fig. 1 A, arrows). Examination of neural crest cells from the N-cadherin–deficient embryos surprisingly showed no detectable change in the abundance of Cx431 gap junction plaques at the cell surface (Fig. 2 A). The specificity of the Cx431 antibody was demonstrated by a parallel analysis of Cx431-deficient neural crest cells, which showed no significant immunostaining (Fig. 2 C). We also examined the expression of N-cadherin in the Cx431-deficient neural crest cells and showed no obvious change in the distribution of N-cadherin, which remained abundant at regions of cell–cell contact (data not shown).
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Figure 1. Colocalization of Cx431 with N-cadherin and ?-catenin in neural crest cells. Neural crest cells in explant cultures derived from the dorsal neural fold of E8.5 mouse embryos were double immunostained with antibodies to Cx431 and N-cadherin (A) or Cx431 and ?-catenin (B). The Cx431 antibody was detected using a FITC secondary antibody (seen in green), whereas the N-cadherin and ?-catenin antibodies were detected using a Cy3 secondary antibody (seen in red). In regions of cell–cell contact, Cx431 is often colocalized with N-cadherin (A) or with ?-catenin (B) (see yellow regions denoted by white arrows in A and B). This is particularly prominent in extended cell processes, some of which are very thin and barely detectable by phase–contrast microscopy. These images were obtained by merging the darkfield FITC and Cy3 immunofluorescence images with the phase–contrast image to help visualize the distribution of Cx43 and N-cadheirn or ?-catenin with regions of cell–cell contact along cell processes. All images are at the same magnification. Bar, 25 μm.: O" _  p! _# z8 ]

3 s7 d* E8 B# [1 dFigure 2. Cx431 and ?-catenin expression in N-cadherin– and Cx431-deficient neural crest cells. Neural crest cells in explant cultures derived from Cx431 and N-cadherin knockout mouse embryos were examined for the expression of Cx431 (A and C) or ?-catenin (B and D) by immunofluorescence microscopy. N-cadherin–deficient neural crest cells show abundant expression of Cx431 gap junctions at regions of cell–cell contact (A), whereas no expression was detected in neural crest cells derived from the Cx431 knockout mouse embryos (B). In the N-cadherin–deficient neural crest cells, ?-catenin expression at the cell surface was reduced compared with wild-type neural crest cells (not shown; Fig. 1 B) and neural crest cells from the Cx431 knockout mouse embryos (D). These images were obtained by merging darkfield fluorescence and phase–contrast images. All images are at the same magnification. Bar, 25 μm.
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Gap junction communication in neural crest cells requires N-cadherin function
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2 l4 N3 A" p" o( [2 GTo determine whether gap junction communication may be functionally altered by the loss of N-cadherin, we quantitated the level of dye coupling. This entailed carrying out microelectrode impalements into neural crest cells and iontophoretically injecting carboxyfluorescein, a fluorescent tracer that can pass through gap junction channels. After 2 min of dye injection, the electrode was withdrawn and the number of dye-filled cells counted to determine the extent of gap junction communication. Such studies carried out in neural crest outgrowth cultures showed that gap junction communication is reduced greatly in the N-cadherin–deficient neural crest cells to levels similar to that observed in the Cx43-deficient neural crest cells (Table I; Huang et al., 1998a). To determine whether the changes in dye coupling detected in vitro reflected changes in gap junction communication in vivo, we examined dye coupling in presumptive neural crest cells in the living embryo. For these studies, intracellular impalements were carried out into the dorsolateral region of the hindbrain neural fold, a region where cardiac neural crest cells are situated. Such studies confirmed that gap junction communication in the N-cadherin–deficient neural crest cells is reduced in vivo as in vitro (Table I). These observations in conjunction with the findings above would suggest that N-cadherin may have a role in regulating gating of the Cx431 gap junction channel.
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Table I. Dye coupling in neural crest cells of N-cadherin
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N-cadherin deficiency reduces cell migration and elevates cell proliferation
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+ {1 X; A  h8 Y4 LGiven our previous observations showing a reduction in the rate of neural crest cell migration with the loss or reduction of Cx431-mediated gap junction communication, we expected that the loss of N-cadherin would reduce the rate of neural crest cell migration. To monitor the overall rate of neural crest cell migration, we first examined the migration index, a method for estimating migration rate based on a measurement of the neural crest outgrowth area in neural tube explant cultures (Huang et al., 1998a). In the 24-h outgrowth cultures, the migration index was reduced significantly in the heterozygous and homozygous N-cadherin knockout mouse embryos. However, by 48 h no significant migration differences were detected (Table II). Analysis of BrdU incorporation suggests that this disparity could be due to alterations in the rate of cell proliferation. Thus, cell proliferation was significantly increased in the heterozygous and homozygous N-cadherin knockout explants (Table III), which over 48 h could inflate the migration index (Table II). It is interesting to note that these results contrast with that of the Cx431-deficient neural crest cells, which do not show any change in the rate of cell proliferation (Huang et al., 1998a).
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0 {' e5 {4 J; \" \, E/ u! xTable II. Neural crest migration in neural tube explant cultures from N-cadherin knockout mouse embryosa6 N" t& O% K0 o; o3 C

; C" E( q( |$ o5 t; Q; nTable III. Neural crest cell proliferation in explant cultures of N-cadherin knockout mouse embryosa
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8 u/ d& x+ H2 q' SNeural crest cell motility differentially affected by N-cadherin versus Cx431 deficiency  n+ L8 {+ M; z( U. M" F* ?
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To determine precisely how cell motility is affected by the loss of N-cadherin and how it may compare with cell motility perturbation elicited by Cx431 deficiency, we used time-lapse videomicroscopy and motion analysis to quantitate various cell motility parameters in individual neural crest cells. For this study, images of neural tube explant cultures were captured every 10 min over a 20-h interval. Examination of the resulting time-lapse movies revealed no discernible difference in the timing of neural crest cell emergence in explants derived from the N-cadherin– or Cx431-deficient mouse embryos compared with wild-type littermates. Tracking and analyzing the migratory paths of individual neural crest cells provided quantitative information on three cell motility parameters: speed, directionality (ratio of net over total distance traveled), and persistence of cell movement (direction change divided by speed). Surprisingly, this analysis showed that the speed of neural crest cell locomotion was elevated in the N-cadherin–deficient neural crest cells, but this was accompanied by a decrease in the directionality of cell movement (Table IV). This reduction in directionality is sufficiently large enough that it can be discerned visusally by the actual migratory paths of the individual neural crest cells (Fig. 3) . In addition, the persistence of cell movement was increased in the N-cadherin–deficient neural crest cells (Table IV; data not shown). A parallel analysis of Cx431-deficient neural crest cells revealed a similar reduction in the directionality of cell movement but no change in the speed or the persistence of cell movement (Table V). These results suggest that the altered cell motility of the N-cadherin–deficient neural crest cells is not simply due to the perturbation of Cx431-mediated gap junction communication.
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Table IV. Analysis of N-cadherin–deficient neural crest cell locomotion by time-lapse videomicroscopy. u4 N8 ]" u8 |1 _) n
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Figure 3. Migratory paths of individual neural crest cells captured by time-lapse videomicroscopy. The color lines represent the migratory path of individual neural crest cells in a neural tube explant culture obtained with images captured every 10 min over 20 h. The circle represents the original position of each cell as it emerged from the neural tube explant. The migratory paths of neural crest cells derived from the N-cadherin–deficient embryo (right explant) are more tortuous than that of the wild-type embryo (left explant). Note that the neural tube explant flattened over time, thereby giving the erroneous impression that some of the neural crest cells originated from deep within the explant. Bar, 100 μm.
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, x2 E) o8 _+ W4 P5 [( mTable V. Analysis of Cx431-deficient neural crest locomotion by time-lapse videomicroscopy* C9 [6 b$ J2 _6 c. R# V* ^& n/ Z3 Y
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Wnt1 is essential for coupling but not neural crest cell motility( V* @) l( |' q
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Since changes in cadherin expression can affect ?-catenin distribution (Dufour et al., 1999), we examined ?-catenin expression in neural crest cells by immunofluorescence microscopy. These studies are of particular interest, given a recent report showing that wnt1-Cre–mediated deletion of ?-catenin in the dorsal neuroepithelium and neural crest cells resulted in craniofacial defects indicative of cranial neural crest perturbations (Brault et al., 2001). In the N-cadherin–deficient neural crest cells, we observed a marked reduction in cell surface expression of ?-catenin at regions of cell–cell contact in extended cell processes (Fig. 2 B compare with Fig. 1 B). As Cx431 also has been shown to bind ?-catenin (Ozawa et al., 1989; Ai et al., 2000), we carried out ?-catenin and Cx431 double immunolocalization studies. In wild-type neural crest cells, ?-catenin was expressed abundantly and exhibited extensive colocalization with Cx431 (Fig. 1 B). In the absence of Cx431, ?-catenin continued to be expressed abundantly at regions of cell–cell contact, similar to that seen in wild-type neural crest cells (Fig. 2 D; data not shown). Due to technical constraints, we were unable to determine whether there were any changes in the level of ?-catenin expression in the cytoplasm or nucleus.0 p8 M2 a1 \2 r$ S, R
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These results suggest the possibility that N-cadherin deficiency could alter ?-catenin–mediated wnt signaling in neural crest cells. Previous studies have indicated a role for wnt signaling in neural crest cell proliferation and differentiation, but whether there is also a role in cell migration is not known (Ikeya et al., 1997; Dunn et al., 2000). To examine this question, we analyzed the motility of neural crest cells from the wnt1 knockout mouse embryos. Using time-lapse videomicroscopy and motion analysis, we found no change in any cell motility parameter with the loss of wnt1 function (Table VI). We also used lithium treatment to mimic wnt signaling in neural tube explant cultures and at 10 mM concentration (the highest compatible with viability) found no significant effect on neural crest cell motility (data not shown). These results indicate that wnt1 does not have an essential role in neural crest cell motility.
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Table VI. Analysis of wnt1-deficient neural cell locomotion by time-lapse videomicroscopya
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% h8 Y: S# x7 B; P& S  GSince previous studies by others have indicated that wnt1 signaling potentially can modulate gap junction communication (Olson et al., 1991; Olson and Moon, 1992) and Cx431 transcription (van der Heyden et al., 1998; Ai et al., 2000), we also examined Cx431 gap junction expression and dye coupling in the wnt1 knockout mouse embryos. Analysis of the wnt1-deficient neural crest cells by immunohistochemistry revealed an abundance of Cx431 gap junctions (Fig. 4 A). N-cadherin expression also was not detectably altered (Fig. 4 B) nor was the cell surface expression of ?-catenin (data not shown). Nevertheless, analysis of dye coupling with microelectrode impalements into presumptive neural crest cells in E8.5 embryos showed that gap junction communication was reduced greatly in the wnt1 knockout embryos to a level similar to that of the Cx431 or N-cadherin knockout mouse embryos (Table VII) (Huang et al., 1998a). These results indicate that although wnt1 is not required for Cx431 protein expression nor Cx431 gap junction formation, it nevertheless may have a role in regulating gating of the Cx431 gap junction channel.
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Figure 4. Expression of Cx431 gap junctions in neural crest cells from the wnt1 knockout mouse embryos. Immunodetection with a Cx431 antibody show abundant Cx431 expression associated with the cell periphery and extending cell processes in migrating neural crest cells derived from the wnt1-deficient mouse embryo (A). Double immunostaining (B) with a N-cadherin (red) and Cx431 antibodies (green) showed some regions of cell–cell contact with N-cadherin and Cx431 colocalization (yellow), a pattern similar to that seen in wild-type neural crest cells. These images were obtained by merging the darkfield fluorescence and phase–contrast images. All images are at the same magnification. Bar, 25 μm.
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7 S. n- \4 u. ~# \. t( aTable VII. Dye coupling in neural crest cells from the wnt1 knockout mouse embryosa
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. ?1 U; X$ x2 R. s3 \) |A possible role for p120 catenin in neural crest cell motility/ i' Z7 B$ p4 ?: u

6 p$ w% K& S2 [- cTo further investigate the mechanism by which N-cadherin and Cx431 gap junctions may modulate neural crest cell motility, we considered the potential role of p120ctn, an Armadillo protein which binds to the juxtamembrane region of cadherin and is kown to modulate cell motility through the Rho GTPases (for review see Anastasiadis and Reynolds, 2000). Studies using various tissue culture cells have shown that p120ctn can be found distributed between the cell membrane, cell cytoplasm, and the nucleus (Anastasiadis and Reynolds, 2000). Analysis by immunohistochemistry showed that p120ctn is expressed abundantly in mouse cardiac neural crest cells. It is membrane localized at regions of cell–cell contact, and it is also present diffusely in the cell cytoplasm (Fig. 5 B). When the neural crest cells were fixed in the presence of 0.3% Triton X-100 to extract the cytoplasmic p120ctn, localizaton of p120ctn in the nucleus also can be discerned (Fig. 5 E and Fig. 6 A). The level of nuclear staining varied somewhat between explants with most being at very low levels (Fig. 5 E and Fig. 6 B). However, in 1 of 15 wild-type neural crest explants examined a more prominent level of nuclear staining was observed (Fig. 6 A). The inclusion of Triton in the fixative also made the membrane-bound p120ctn more sharply delineated, even when viewed by standard immunofluorescence microscopy (see Fig. 5 E, with Triton treatment; compare with B, without Triton treatment). This is presumably due to the removal of cytoplasmic p120ctn. It is important to note that the overall pattern of p120ctn distribution at the cell surface in Triton-treated cells was identical to that seen in cells fixed without Triton, being associated mostly with regions of cell–cell contact. Note that Fig. 5 B shows a relatively low level of cytoplasmic p120ctn immunostaining, since the image was obtained from optical slices of only 0.2 μm in thickness. In contrast, Fig. 5 E is a standard immunofluorescent image, but most of the cytoplasmic p120ctn has been extracted due to the inclusion of Triton during fixation.
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Figure 5. Neural crest cells show colocalization of N-cadherin and Cx431 with p120ctn. Neural crest cells derived from wild-type mouse embryos were double immunostained using Cx431 and p120ctn antibodies (A–C) or N-cadherin and p120ctn (D–F) antibodies. (A–C) Images of Cx431/p120ctn double immunostained cells were obtained by iterative deconvolution analysis of image stacks comprised of 0.2-μm slices. Merging of the Cx431 (A) and p120ctn (B) immunofluorescence images in C showed that Cx431 and p120ctn are extensively colocalized along cell processes (C, arrows). (D–F) For the N-cadherin/p120ctn immunostaining, the cells were fixed in the presence of 0.3% Triton X-100, and the sections were viewed by standard immunofluorescence microscopy. The removal of much of the cytoplasmic p120ctn by Triton extraction allowed clear delineation of the cell surface localized p120ctn and N-cadherin without deconvolution analysis. Note the colocalization of p120ctn and N-cadherin shown in F by merging the p120ctn (D) and N-cadherin immunofluorescence images (E) with a phase–contrast image of the cells. Note that some p120ctn immunostaining can be observed in the nucleus in the Triton extracted cells (E). All images are at the same magnification. Bar, 25 μm.& W' a+ \0 Y) B# _1 U+ z4 X- O
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Figure 6. Immunolocalization of p120ctn in N-cadherin– and Cx431-deficient neural crest cells. Neural crest cells from wild-type, Cx431, and N-cadherin knockout mouse embryos were fixed in the presence of Triton X-100 and then immunostained with a p120ctn antibody. In wild-type neural crest cells, p120ctn localized predominantly at the cell periphery, outlining regions of cell–cell contact found in cell processes (A and B). In such Triton extracted cells, some nuclear localization of p120ctn can be observed (A). In neural crest cells from the Cx431-deficient embryos (C), p120ctn expression in the cell periphery is reduced, and there is increased localization of p120ctn in the nucleus. In the N-cadherin–deficient neural crest cells (D), p120ctn expression at the cell periphery is generally not observed except in some cell processes. However, there is very strong localization of p120ctn in the nucleus. These images were obtained by standard immunofluorescence microscopy. All images are at the same magnification. Bar, 25 μm.' U8 T, X% H7 X7 l
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To determine whether N-cadherin and p120ctn are colocalized at the cell surface, immunostaining was carried out using an N-cadherin mouse monoclonal antibody (Fig. 5 D) followed by an FITC- conjugated p120ctn mouse antibody (Fig. 5 E). The N-cadherin antibody binding was detected using Cy3-conjugated secondary antibody. This analysis showed extensive colocalization of N-cadherin with p120ctn at the cell surface (Fig. 5 F). Interestingly, double immunostaining of neural crest cells with Cx431 and p120ctn antibodies showed that Cx431 is also extensively colocalized with p120ctn at the cell surface (Fig. 5 C, white arrows). This same pattern of p120ctn colocalization with N-cadherin and Cx431 was observed in cells fixed with or without Triton (Fig. 5; data not shown).* y% p3 V! V( W: t7 T! Q# Q: |- B
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In the N-cadherin– and Cx431-deficient neural crest cells, we observed distinct changes in the pattern of p120ctn distribution. In the N-cadherin–deficient neural crest cells, most of the cell surface expression of p120ctn was lost; although infrequently, some p120ctn can still be found along cell processes (Fig. 6 D). Very striking was the strong localization of p120ctn in the nuclei of the N-cadherin–deficient crest cells (Fig. 6 D). The increase in p120ctn immunostaining in the nuclei cannot be fully appreciated by the image in Fig. 6 D, since the immunofluorescence intensity was beyond the saturation limit of the camera. A parallel analysis of the Cx431-deficient neural crest cells also revealed an apparent increase in p120ctn localization in the nucleus but not as striking as that observed in the N-cadherin knockout crest cells (Fig. 6 C). In addition, there was a reduction in cell surface expression of p120ctn (Fig. 6 C compare with A and B). Quantitative analysis showed that this is due to a reduction in both the length and area of cell surface p120ctn (Table VIII). Overall, these observations suggest that the loss of N-cadherin and Cx431 could potentially perturb p120ctn-mediated signaling in neural crest cells.0 Q3 f- y2 }2 Q  z" x. A4 j

: V' Q  j; q7 v/ @$ t5 rTable VIII. Reduction of cell surface expression of p120ctn in Cx43a1 knockout neural crest cells* u2 O1 t$ ~' Q1 f8 p# O) Z
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Discussion
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* [0 F6 |3 ]: `Our studies showed that N-cadherin is expressed in cell processes extended between migrating mouse cardiac neural crest cells. Often N-cadherin was colocalized or closely juxtaposed with Cx431 gap junctions. In the N-cadherin–deficient neural crest cells, Cx431 gap junction contacts remained abundant at the cell surface. This is unexpected, since previous studies in tissue culture cells indicated that cadherin-containing adherens junctions may play an important role in gap junction formation (Keane et al., 1988; Mege et al., 1988; Musil et al., 1990; Jongen et al., 1991; Meyer et al., 1992; Frenzel and Johnson, 1996; Fujimoto et al., 1997). However, it is possible that another cadherin expressed in neural crest cells may help facilitate the formation of Cx431 gap junctions in the absence of N-cadherin (Kimura et al., 1995; Nakagawa and Takeichi, 1995). Despite the prevalence of gap junctions in the N-cadherin–deficient neural crest cells, gap junction communication was nevertheless reduced markedly to levels like that of the Cx431-deficient crest cells. Thus N-cadherin, though not essential for Cx431 gap junction formation, is required for dye coupling via Cx431 gap junction channels.& X4 C, ?* L& R: |
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Our studies also revealed an essential role for N-cadherin in mouse neural crest cell migration. Analysis of neural crest outgrowth area in neural tube explant cultures indicated a significant reduction in the apparent rate of neural crest cell migration in the absence of N-cadherin. These results are similar to that seen previously in Cx431-deficient neural crest cells (Huang et al., 1998a). However, time-lapse videomicroscopy and an analysis of the migratory movement of individual neural crest cells revealed distinct differences in the motility changes elicited by the loss of N-cadherin versus Cx431. N-cadherin–deficient neural crest cells exhibited an increase in the speed of cell locomotion but a significant reduction in the directionality of cell movement. In contrast, Cx431 deficiency elicited only a reduction in the directionality of cell movement but no change in the speed of cell locomotion. Whereas the directionality of cell movement was reduced only in the homozygous N-cadherin and Cx431 knockout neural crest cells, the speed of cell locomotion was elevated in both heterozygous and homozygous N-cadherin knockout neural crest cells, thus indicating the latter cell motility parameter is subject to N-cadherin haploin sufficiency.
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Modulation of neural crest cell motility by N-cadherin+ `: a5 N9 v( X
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Overall, our studies indicated that N-cadherin has an essential role in neural crest cell motility and that this cannot simply involve facilitating Cx431-mediated gap junction communication. We note that although the formation of cadherin-based adhesion contacts are generally associated with the cessation of cell locomotion, N-cadherin can stimulate the motility of breast cancer cells and also has been shown to facilitate tumor cell metastasis (Nieman et al., 1999; Hazan et al., 2000). In addition, ectopic expression of N-cadherin can cause cells to lose their tight cell–cell contact and convert into a spindle or fibroblastic morphology (Kintner, 1992; Islam et al., 1996). Although the precise mechanism by which N-cadherin facilitates cell migration is not known, previous studies have indicated that cadherin's role in cell motility is separable from its role in cell adhesion. Thus, the ?-catenin–binding domain of cadherin required for cell adhesion is not essential for cell motility (Chen et al., 1997). Studies with retinal ganglion cells also indicated that the ?-catenin–binding domain of N-cadherin is not required for cadherin's role in axonal outgrowth (Riehl et al., 1996). Rather, recent studies suggest that cadherin may modulate cell motility by mediating signaling via the p120ctn family of Armadillo proteins. p120ctn is bound to cadherin's juxtamembrane region, a domain distinct from that mediating ?-catenin binding (Yap et al., 1998; Anastasiadis et al., 2000). Studies in various cultured cell lines show that p120ctn is also found in the cytoplasm, where it can modulate the activity of the RhoGTPases and thus affect cell motility by changing the organization of the actin cytoskeleton (Anastasiadis et al., 2000; Grosheva et al., 2000; Noren et al., 2000). In addition, p120ctn can signal to the nucleus, since it has been shown to interact with the transcription factor Kaiso and translocate into the nucleus (Daniel and Reynolds, 1999)." S1 j( U3 m: ~. {/ k6 `  n

" `; S' S/ M) N# wWe observed that p120ctn is expressed abundantly in migrating mouse cardiac neural crest cells. Its distribution is similar to that described for other cell types: being localized at the membrane, diffusely in the cytoplasm, and at a low level in the nucleus. The latter was most evident when the heavy cytoplasmic p120ctn immunostaining was reduced by including Triton in the fixative. In the N-cadherin–deficient neural crest cells, membrane-bound p120ctn was decreased, whereas p120ctn localization in nuclei was markedly increased. These results are consistent with a previous study showing that the exogenous expression of E-cadherin can downregulate the nuclear localization of p120ctn (Van Hengel et al., 1999). Since the Rho GTPases also have been shown to have a role in cell cycle regulation (Welsh and Assoian, 2000; Assoian and Schwartz, 2001), alterations in p120ctn signaling could potentially affect cell proliferation in the N-cadherin–deficient neural crest cells. We note that ectopic N-cadherin expression in breast tumor cells was reported to slow the growth of individual tumors, a result that is consistent with our finding of an elevation of cell proliferation in the N-cadherin–deficient neural crest cells (Hazan et al., 2000).
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8 ^, x! w2 {# h4 M& q0 I1 i2 E7 pThe role of connexins in neural crest cell motility
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% J3 O. I, T3 P4 qThe present study together with our earlier work showed that Cx431 gap junctions play an essential role in neural crest cell motility. We proposed previously that Cx431-mediated gap junction communication might regulate neural crest cell migration by facilitating the cell-to-cell movement of second messengers and other cell-signaling molecules involved in cell locomotion (Huang et al., 1998a; Lo and Wessels, 1998). This model was based on our finding of an apparent correlation between changes in the level of dye coupling and alterations in the apparent rate of neural crest cell migration elicited by the gain or loss of Cx431 function. However, in the present study we showed that neural crest cells from the wnt1 knockout mouse embryos have normal cell motility even though they exhibited only a low level of functional coupling via gap junction channels. In addition, our studies showed no consistent correlation between cell motility changes and the stepwise decrease in dye coupling in neural crest cells derived from the heterozygous and homozygous N-cadherin or Cx431 knockout mouse embryos. This divergence between changes in neural crest cell motility and alterations in the level of dye coupling would suggest that carboxyfluorescein injection is not an appropriate method for examining the role of Cx431 in cell motility. Thus, carboxyfluorescein may not be a suitable gauge of the movement of molecules that are important in cell motility. Since wnt1-deficient neural crest cells continued to exhibit a low level of residual dye coupling, in principle this may be sufficient to mediate the cell-to-cell movement of molecules involved in modulating cell motility. However, this explanation is somewhat problematic, given that Cx431- and N-cadherin–deficient neural crest cells have dye coupling levels comparable to that of the wnt1-deficient neural crest cells.
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Another possibility to consider is that the role of Cx431 in motility may involve a novel function. Since Cx431 is extensively colocalized with p120ctn, perhaps connexins like cadherins may have a role in modulating p120ctn signaling. It is of significance to note that the distribution of p120ctn is altered in the Cx431-deficient neural crest cells. In the wnt1-deficient neural crest cells, Cx431 gap junctions continued to be expressed in abundance at the cell surface. Since Cx431 gap junctions are also maintained in N-cadherin–deficient neural crest cells, presumably it is not merely whether Cx431 gap junctions are present but perhaps the modulation of Cx431 interaction with other proteins that is of critical importance in cell motility.8 G) h7 x6 Q/ C' n+ N

; X8 `8 O. ]7 p; `& L8 C9 _0 e, Y( JThe integration of Cx431 and N-cadherin in cell motility
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& Y5 X9 U& ^3 N1 W+ U" NIn light of the binding of p120ctn by Cx431 and N-cadherin and the colocalization or juxtapositioning of Cx431 and N-cadherin at the cell surface, we hypothesize that Cx431 and N-cadherin may be integrated within a cell surface complex that can modulate cell motility by transducing signals to the cytoskeleton and the nucleus. This would allow cell adhesion contacts and gap junctions to be regulated dynamically in conjunction with cell locomotion. Since p120ctn family members are subject to differential splicing and are typically expressed in multiple isoforms (Reynolds, et al., 1994; Staddon et al., 1995; Mo and Reynolds, 1996; Keirsebilck et al., 1998), it is possible that different p120ctn isoforms may bind Cx431 versus N-cadherin and thus differentiate the roles of cadherin versus connexin in cell motility. In preliminary studies, we observed that Cx431 coimmunoprecipitated with only one of two p120ctn bands expressed in NIH3T3 cells (unpublished data). In future studies, it may be interesting to examine another p120ctn family member, Armadillo repeat protein deleted in velocardiofacial syndrome (ARVCF; Mariner et al., 2000), a gene situated in a chromosome region deleted frequently in patients with velocardiofacial syndrome, a congenital condition involving cranial and cardiac neural crest defects (Young et al., 1980; Shprintzen et al., 1981; Sirotkin et al., 1997). Studies in polarized epithelial cells have indicated that scaffold proteins play an important role in integrating components of the apical junctional complexes with signal transduction pathways (Yeaman et al., 1999). In this regard, it is interesting to note that the scaffold protein ZO-1 has been shown to bind Cx431 (Toyofuki et al., 1998); ZO-1 also has been observed to have a role in cadherin-based cell adhesion (Itoh et al., 1997). In future studies, it will be important to examine, on a biochemical level, the interactions of Cx431 and N-cadherin and their interactions with ZO-1, p120ctn, and other cytoplasmic proteins. However, these studies will likely necessitate the use of model tissue culture systems, since at best only a few thousand neural crest cells can be obtained from each postotic neural tube explant culture.$ U. y3 j4 E1 S
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Modulation of gap junction communication by N-cadherin and wnt1
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Our finding that Cx431 gap junctions persisted even as dye coupling was virtually eliminated in the N-cadherin– and wnt1-deficient neural crest cells would suggest that wnt1 signaling and N-cadherin can modulate gating of the Cx431 gap junction channel. These results contrast with a previous study of mammalian tissue culture cells, indicating that Cx431 is transcriptionally regulated by the wnt1/?-catenin–signaling pathway (van der Heyden et al., 1998). Studies in the Xenopus embryo have shown that wnt signaling can modulate gap junction communication (Olson et al., 1991; Olson and Moon, 1992; Heasman et al., 1994), but this likely is posttranscriptionally regulated, since the effects of wnt on coupling occurs before the activation of zygotic transcription. It is interesting to note that more recent work suggests that the wnt modulation of gap junction communication in Xenopus embryos involves ?-catenin but is independent of cadherin's role in cell adhesion (Krufka et al., 1998). As ?-catenin is extensively colocalized with Cx431 in neural crest cells, an interesting possibility is that the inhibition of dye coupling in the wnt1- and N-cadherin–deficient neural crest cells may arise from the perturbation of ?-catenin and Cx431 interactions. For example, ?-catenin interactions could modulate conformation of the cytoplasmic tail of Cx431 and thus affect gating of the gap junction channel as proposed in the particle–receptor model (Homma et al., 1998). We note that the cell surface localization of ?-catenin is greatly reduced in both wnt1- and N-cadherin–deficient neural crest cells. A possible role for p120ctn in gating the gap junction channel also may be worth considering. In addition, as the cytoplasmic tail of Cx431 contains a consensus phosphorylation site for GSK3? (Lau et al., 1996), alterations in wnt signaling could alter phosphorylation of the cytoplasmic tail of Cx431 and thus bring about conformational changes that may perturb channel activity directly or indirectly. In future studies, it will be important to define the cytoplasmic domain of Cx431 to which ?-catenin and p120ctn binds and determine whether mutations that disrupt such interactions may affect gating of the gap junction channel.( o/ ~8 w0 g! x& [7 v* v( t1 j
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Materials and methods0 ~$ ^, w3 |- c/ g* E
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Mouse breeding and genotyping
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, e! C8 z! s1 p2 uHeterozygous N-cadherin, Cx431, and wnt1 knockout mice were interbred to generate litters of embryos that were then genotyped by PCR analysis of yolk sac DNA (Hanley and Merlie, 1991). The oligonucleotide primers and thermal cycler conditions used for PCR genotyping the wnt1 knockout mice are as described previously (McMahon et al., 1992). For genotyping the Cx431 knockout mouse embryos, the primers and conditions used are modified from those previously described (Reaume et al., 1995). For detecting the wild-type allele, the primer pairs used consisted of IMR5-5'-ACTTTTGCCGCCTAGCTATCCC-3' and IMR3-5'-CCCCACTCTCACCTATGTCTCC-3', which gave a 500-bp product, wheras the knockout allele was detected using primers IMR5-5'-ACTTTTGCCGCCTAGCTATCCC-3' and NEO5-5'-GCTTGCCGAATATCATGGTGG A-3', which gave a 1,000-bp product. The cycling parameters were 94°C for 45 s, 61.5 (wild-type allele) or 60°C (knockout allele) for 30 s, and 72°C for 1 min for 35 cycles. For the N-cadherin knockout mouse embryos, two primer pairs were used: one set detected the N-cadherin knockout allele corresponded to the neo gene, 5'-CGTGTTCCGGCTGTCAGCGCAGG-3' and 5'-CAACGCTATGTCCTGATAGCGGTCC-3', and the other primer pairs detected the wild-type N-cadherin allele corresponded to exon 10 of the N-cadherin gene, 5'-CAGCCAATTGACTTTGAAACG-3' and 5'-GGCGAATGATTTTAGGATTTGGGG-3'. Cycling parameters were 94°C for 1 min, 65°C for 2 min, and 72°C for 3 min for 30 cycles. PCR products were resolved on a 1.0–1.6% agarose gel.
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. r% a' H# L, p$ @  R0 g3 kNeural tube explant cultures
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To obtain embryos for neural crest outgrowth studies, mice were mated, and the day the vaginal plug was found was considered E0.5. Embryos were collected on E8.5, and the yolk sac from each embryo was harvested for genotyping by PCR analysis. The method used to establish the neural crest outgrowth cultures was described previously (Huang et al., 1998a) and was adapted from those described by Murphy et al. (1991) and Moiseiwitsch and Lauder (1994). In brief, the hindbrain neural folds of E8.5 embryos were collagenase/dispase treated, and the dorsal ridge of the neuroepithelium spanning the postotic region of the hindbrain neural fold was surgically removed from the surrounding tissue and cultured on a fibronectin-coated Petri dish in DME with high glucose and 10% FBS. The cultures were maintained for 24–48 h at 37°C with 5% CO2. Lineage studies in mouse embryos have confirmed that neural crest cells from the postotic hindbrain neural fold give rise to the cardiac crest cells, as has been found in chick embryos (Fukiishi and Morriss-Kay, 1992; for review see Kirby and Waldo, 1995).
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6 o1 B* v5 R! `5 v* A1 G( N! ]Neural crest dye coupling analyses in vitro and in vivo
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To quantitate gap junction communication, dye coupling experiments were carried out in vitro and in vivo as described previously. For the in vitro studies, microelectrode impalements were made into neural crest cells in 24-h explant cultures (Huang et al., 1998a). Microelectrodes were filled with 5% carboxyfluorescein and dye was iontophoretically injected for 2 min. The number of dye-filled cells found at the termination of dye injection was recorded. To examine dye coupling in vivo, impalements and dye injections were carried out into the dorsolateral region of the postotic hindbrain neural fold, the region where presumptive cardiac crest cells are found, and the number of dye filled cells recorded after 2 min as described above.! A1 L  c  A: C' N7 U
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Analyses of neural crest cell migration and proliferation$ \8 v4 Z1 z2 Z1 S( d9 a1 v
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Images of the neural tube explant cultures were acquired by videomicroscopy at 24 and 48 h, and the outgrowth area was measured as described previously (Huang et al., 1998a). In brief, this entailed using NIH Image software to measure the area and perimeter of the outgrowth to obtain the migration index, which is the outgrowth area (mm2) divided by the perimeter (mm) of the explant. This normalized for variations in the outgrowth area arising from differences in the shape of the explant. For monitoring cell proliferation, 48-h explant cultures were incubated with BrdU (10 μM/ml) for 2 h, then fixed, and processed for immunodetection using an anti-BrdU antibody as described previously (Huang et al., 1998a). To determine the total cell number in the outgrowth after BrdU immunostaining, the cultures were further stained with hematoxylin. Cell counting was carried out using NIH Image. The migration and proliferation obtained were analyzed statistically using Statview (SAS Institute, Inc.).5 A- d- D+ y  r7 T' q3 T3 k

0 \4 d& E" ~" W0 X2 W( o/ ]# e& vTime-lapse videomicroscopy and motion analysis, L1 P5 J# G% ~! A
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For time-lapse videomicroscopy, images of the explants were captured every 10 min over a 20-h interval using IPLab (Scanalytics) or Openlab (Improvision, Inc.) imaging software. For these studies, the explant cultures were maintained at 37°C on a heated microscope stage in phosphate-buffered L-15 medium containing 10% FBS. The images were then converted to a Quicktime movie for viewing and motion analysis. The motion analysis was carried out using the Dynamic Image Analysis Software (Solltech, Inc.). Using this software, we determined the speed of cell locomotion, the directionality of cell movement (the net distance achieved divided by the total distance traveled), the direction change (the change in direction of cell movement in degrees), and the persistence of cell movement (the speed of cell locomotion divided by direction change in gradients). The data obtained was statistically evaluated by ANOVA using Statview (SAS Institute, Inc.).
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; b) G" h9 N* r( ~8 C$ S8 G, jImmunohistochemistry& V# s- v2 [( u- E! J
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For immunohistochemical staining of neural crest cells in explant cultures, neural tube explants were plated on glass coverslips coated with 15 μg/ml of fibronectin. After 24–48 h of culture, the cells were fixed in 4% paraformaldehyde in PBS for 20 min at room temperature. In some cases, cells were first incubated with 3.7% formaldehyde containing 0.3% Triton X-100 for 3 min followed by postfixation in 4% paraformaldehyde for an additional 15 min. For immunostaining, the coverslips were incubated for 2 h at room temperature with the primary antibody diluted in blocking buffer (1% BSA and 2% FBS with 0.3% Triton X-100 in PBS), then washed with PBS three times for 1 h or longer, and incubated with the secondary antibody for 45 min at room temperature. After washing, the coverslips were mounted with SlowFade antifade reagent (Molecular Probes) on glass slides and examined by epifluorescence illumination using a 40 or 63x oil objective on a Leica DMRE microscope. All of the images were captured using a 5 Mhz Micromax cooled CCD camera. For some samples, images were obtained as Z stacks comprised of 0.2-μm optical slices and then subjected to deconvolution analysis by volume neighbor or iterative deconvolution algorithms using the Openlab deconvolution software (Improvision, Inc.).
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( t; K6 e, r/ g0 C' ~4 u- zThe antibodies used included mouse monoclonal anti–N-cadherin antibody from Zymed Laboratories (33-3900; used at 1:100 dilution), mouse monoclonal anti–?-catenin IgG (C19220; used at 1:100 dilution), mouse monoclonal anti-p120ctn antibody (P17920; 1:500 dilution), and mouse monoclonal anti–p120-FITC–conjugated antibody from BD Transduction Laboratory (P17924; 1:50 dilution), and rabbit polyclonal Cx431 antibody 18-A8 made against the cytoplasmic tail of Cx431 (1:1500 dilution; supplied by Dr. Elliot Hertzberg, Albert Einstein College of Medicine, Bronx, NY). The secondary antibodies used were Cy3- or FITC-conjugated goat anti–mouse or anti–rabbit IgG obtained from Jackson ImmunoResearch Laboratories. For double immunostaining with the N-cadherin and p120ctn mouse antibodies, cells were first incubated with the N-cadherin antibody followed by Cy3-conjugated anti–mouse antibody. Then they were further incubated with the FITC-conjugated p120ctn antibody.; T  |: T( n6 f; h8 R  M

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希瑞干细胞
拿分走人呵呵,楼下继续!
佰通生物

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干细胞之家微信公众号
长时间没来看了 ~~  

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你加油吧  

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不知道说些什么  

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留个脚印```````  

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今天临床的资料更新很多呀

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小生对楼主之仰慕如滔滔江水连绵不绝,海枯石烂,天崩地裂,永不变心.  

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谢谢哦  
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