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Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours  
9 n5 c' ?7 y: p; y/ V) U! B  h作者: Rijlaarsdam, MA (Rijlaarsdam, M. A.)1; van Herk, HADM (van Herk, H. A. D. M.)1; Gillis, AJM (Gillis, A. J. M.)1; Stoop, H (Stoop, H.)1; Jenster, G (Jenster, G.)2; Martens, J (Martens, J.)3; van Leenders, GJLH (van Leenders, G. J. L. H.)1; Dinjens, W (Dinjens, W.)1; Hoogland, AM (Hoogland, A. M.)1; Timmermans, M (Timmermans, M.)2; Looijenga, LH (Looijenga, L. H. J.)1  ' k  w; l" [( J9 |, k
来源出版物: BRITISH JOURNAL OF CANCER  卷: 105   期: 6   页: 854-863   DOI: 10.1038/bjc.2011.270   出版年: SEP 6 2011  
5 l8 e# k; Z; x: @8 p8 |6 D# e* \9 J被引频次: 0 (来自 Web of Science)  ) ]" Y& p# f6 d2 k/ K" w
引用的参考文献: 52 [ 查看 Related Records ]     引证关系图      
1 a  j$ P: P* F2 n6 h. V6 E# w摘要: BACKGROUND: OCT3/4 (POU5F1) is an established diagnostic immunohistochemical marker for specific histological variants of human malignant germ cell tumours (GCTs), including the seminomatous types and the stem cell component of non-seminomas, known as embryonal carcinoma. OCT3/4 is crucial for the regulation of pluripotency and the self-renewal of normal embryonic stem-and germ cells. Detection of expression of this transcription factor is complicated by the existence of multiple pseudogenes and isoforms. Various claims have been made about OCT3/4 expression in non-GCTs, possibly related to using nonspecific detection methods. False-positive findings undermine the applicability of OCT3/4 as a specific diagnostic tool in a clinical setting. In addition, false-positive findings could result in misinterpretation of pluripotency regulation in solid somatic cancers and their stem cells. Of the three identified isoforms - OCT4A, OCT4B and OCT4B1 - only OCT4A proved to regulate pluripotency. Up until now, no convincing nuclear OCT4A protein expression has been shown in somatic cancers or tissues.
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