StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子
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. i- a6 j; f8 OStemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。0 p3 H& |6 U, T8 M
http://products.invitrogen.com/ivgn/product/10639011' C* W! U1 b9 Q0 N: x# b* d) \9 N
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至于STO feeders， 请看相关文献的描述：
6 D* r6 {/ b$ N( q9 g- z  w3 G) K' LSeveral alternative cell lines have been investigated/ z1 M, D: x+ `3 O/ |
for their ability to support existing hESC lines as well4 \% t1 Z$ T) b" U
as being used to derive new hESC lines (Table 1). The5 }& y9 N6 ]! B
mouse embryonic fibroblast cell line,STO, has been used
$ j9 K5 I: r/ c, a  ]' j9 Oto establish nine cell lines from frozen blastocysts and" z/ O4 n+ S; p2 Z
zygotes (Park et al., 2004). The advantage of STO cells
4 W; `9 ?+ z* r$ E6 eover primary cultures of MEFs is that, being immortalized,
$ E: T% z3 u7 v* o+ m* W) Sthey are easy to maintain and propagate. Human
1 H# A- L8 M( E+ w6 e  m; T7 UES cells cultured on this feeder layer exhibited a similar
7 u3 Z  q! ?" r/ O1 g6 H2 Vdoubling time to those on MEFs and expressed surface+ H5 f6 ]$ W! ^! N7 b7 M, }
markers as expected. In addition, prolonged culture did* K" L0 T$ @! r) \7 w  o: L
not lead to abnormal karyotypes. However, Xu et al." p8 u7 T3 p9 o4 v7 F* e  W
(2001) have found increased differentiation when conditioned
- `5 J8 T% P9 s5 X4 emedium (CM) from these cells was used in
7 C" @5 ^# j  t; T0 Q8 @1 p+ vfeeder-free conditions. Thus, the STO line is not a direct" w" Z5 b* m) W; L* i
substitute for MEFs but can reduce some of the work
6 g6 n8 A9 M/ N4 q* T5 Yload regarding MEF isolation and culture.

yangpan521

非常感谢  受教了