StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子
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7 Z; B! E( K, u# v0 UStemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。5 K. W! S+ q) L$ m9 v" v4 F6 h
http://products.invitrogen.com/ivgn/product/10639011& I( p; H% c4 F
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至于STO feeders， 请看相关文献的描述： % M" R: l. y4 \( A
Several alternative cell lines have been investigated
: J% ?, U, V# M" L$ r. e, Tfor their ability to support existing hESC lines as well$ o; F) S  ?5 D6 l$ ^
as being used to derive new hESC lines (Table 1). The
$ P' r! c! L, E* Wmouse embryonic fibroblast cell line,STO, has been used
- Z) h+ Q5 Z% b) u2 fto establish nine cell lines from frozen blastocysts and) m& s% V* L/ n/ c' O7 b
zygotes (Park et al., 2004). The advantage of STO cells
) z! D) _0 D, F( G' Eover primary cultures of MEFs is that, being immortalized,/ }5 B/ d$ d  l& O
they are easy to maintain and propagate. Human+ V% Y% O/ [0 A) j
ES cells cultured on this feeder layer exhibited a similar
/ x9 d( N! R* Q, K( {doubling time to those on MEFs and expressed surface+ W  l7 s. k0 w* k8 o1 Y
markers as expected. In addition, prolonged culture did
. ~1 T0 ^0 K4 E5 w) lnot lead to abnormal karyotypes. However, Xu et al.
1 |( W- J* p+ i# |* Z(2001) have found increased differentiation when conditioned
5 C& n+ V! e0 z  cmedium (CM) from these cells was used in
7 f4 g! c! G( t/ e! ^. hfeeder-free conditions. Thus, the STO line is not a direct& W1 M; \8 q% t) g0 t2 i' W
substitute for MEFs but can reduce some of the work( Y" u- }6 J0 h! V% G5 h+ B
load regarding MEF isolation and culture.

yangpan521

非常感谢  受教了