StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子
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/ j& T" c- N3 m& ^, f, }* g! C: LStemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。1 Q+ i: [) f# q. T; _3 A' j+ \
http://products.invitrogen.com/ivgn/product/10639011
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至于STO feeders， 请看相关文献的描述： 8 o( {; e# K" i# |# A
Several alternative cell lines have been investigated1 ]& i: D4 s# v4 w- [) L
for their ability to support existing hESC lines as well
: A0 Y# b/ A1 ~1 t( L6 y3 G; Yas being used to derive new hESC lines (Table 1). The* t0 ~# o( }: {" x
mouse embryonic fibroblast cell line,STO, has been used
+ p% o& k* g, g! A6 a% Hto establish nine cell lines from frozen blastocysts and
- A3 f( ]# @( \" R6 i4 a( Mzygotes (Park et al., 2004). The advantage of STO cells' T& v+ e: X$ Z' L
over primary cultures of MEFs is that, being immortalized,& s& o/ c: e. |+ Z! ^
they are easy to maintain and propagate. Human
+ x" R' [- L. Y. S# F& E+ cES cells cultured on this feeder layer exhibited a similar/ @1 Y1 t3 {. w7 d/ `
doubling time to those on MEFs and expressed surface/ s" S; [1 Y; i3 h
markers as expected. In addition, prolonged culture did6 f: H4 w5 @4 [. h. c  i
not lead to abnormal karyotypes. However, Xu et al.
( G" u0 F! q, L; t' `* q5 k, S(2001) have found increased differentiation when conditioned
4 i6 |& r; |3 S, Y( |% H, Bmedium (CM) from these cells was used in
$ O3 P8 _  x/ t# E5 D8 ofeeder-free conditions. Thus, the STO line is not a direct% k; u% T) |# S4 R
substitute for MEFs but can reduce some of the work
5 j3 @8 r: z; C8 Cload regarding MEF isolation and culture.

yangpan521

非常感谢  受教了