StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子& Z. Q9 {4 U! ^: U0 v! X, p
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StemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。- R3 h0 `1 b6 o
http://products.invitrogen.com/ivgn/product/10639011
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至于STO feeders， 请看相关文献的描述：
% M, [. o  S! ]3 I5 U; i4 b& X5 [2 USeveral alternative cell lines have been investigated
% ~+ K4 J, N! s2 Vfor their ability to support existing hESC lines as well3 x( v, C2 X0 M
as being used to derive new hESC lines (Table 1). The0 O0 x% r" L+ G0 L. A
mouse embryonic fibroblast cell line,STO, has been used$ j6 e! I& r. h+ k/ F1 O- {' U
to establish nine cell lines from frozen blastocysts and/ [; }& o* }( e" D* u
zygotes (Park et al., 2004). The advantage of STO cells
  r2 S1 C: |# o4 sover primary cultures of MEFs is that, being immortalized,/ q! r9 y$ A( {9 p; y
they are easy to maintain and propagate. Human
  {+ `: Q& Q$ e. ]8 a9 ?' eES cells cultured on this feeder layer exhibited a similar( |: z6 [7 E7 b2 P
doubling time to those on MEFs and expressed surface
4 R* H9 D% ^9 Q+ \. l& d) a* nmarkers as expected. In addition, prolonged culture did# b/ A( V" {9 m- I# g  q
not lead to abnormal karyotypes. However, Xu et al.
: @; {' i6 i. V$ |8 j  j  {. ?(2001) have found increased differentiation when conditioned7 ]2 k" I/ m) ^, ?; S- S
medium (CM) from these cells was used in8 ]; P5 L, w1 _
feeder-free conditions. Thus, the STO line is not a direct
) ?. C- ]: Q- R. F8 P: Asubstitute for MEFs but can reduce some of the work
) Q$ M7 g" U) ^0 Mload regarding MEF isolation and culture.

yangpan521

非常感谢  受教了