大家好，我想培养有核红细胞，请教！！！

yaolinzhang

不知用gibco的STEMPRO-34SFM Complete Medium还是stem cell 的SFEM好？

xieyuhuan

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你好，我想问下你是怎么培养的有核红细胞，我现在也想做这块，求指导，谢谢！

yaolinzhang

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; }" e. {5 u# s0 Y9 U% U1 r5 w之前计划做，但最终没有做。不过我可以给你提供几篇文献题目（我没有原文）：1 j2 C, o: K  L7 v
1.Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells" @$ q* I2 T( {; W4 B
2.Erythroid progenitor cells expanded from peripheral blood without mobilization or preselection: molecular characteristics and functional competence! m0 F& z/ \/ x+ X9 O1 ^# Y
3.Red blood cell production from immortalized progenitor cell line
: Q0 E! M0 K- f/ R4.In vitro proliferation and differentiation of human CD34+ cells from peripheral blood into mature red blood cells with two different cell culture systems  O* P4 K8 i# o& j: N& G0 G: @
5.Ex vivo production of human red blood cells from hematopoietic stem cells: what is the future in transfusion?
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xieyuhuan

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有些文献我看了，就是每个实验室的条件不一样，所用的方法也不一样，目前正在尝试用EB球法做谢谢！