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+ Y. \' f" n; xNature Biotechnology 14, 185 - 188 (1996)
3 C4 o9 l6 R- d& N0 \" h% y/ V- vdoi:10.1038/nbt0296-185
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6 h  |' g3 n; c* N6 ^& cImproved Wool Production in Transgenic Sheep Expressing Insulin-like Growth Factor 12 M: Z0 r+ i# G3 J' a- s
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Sami Damak1, Hung-yi Su1, ,*, Nigel P. Jay2 & David W. Bullock1, ,2, ,*
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Abstract
/ Q" S" i8 p6 \3 m. h9 ?- E3 i2 wTransgenic sheep were produced by pronuclear microinjection with a mouse ultra-high-sulfur keratin promoter linked to an ovine insulin-like growth factor 1 (IGF1) cDNA. Five transgenic lambs resulted from the microinjection of 591 embryos; one male and one female showed IGF1 expression in the skin. A progeny test of the ram was carried out by matings to 43 non-transgenic ewes. Of 85 lambs born, 43 (50.6%) were transgenic. At yearling shearing (approximately 14 months of age), clean fleece weight was on average 6.2% greater in transgenic animals than in their non-transgenic half-sibs, with a greater effect in males (9.2%) than females (3.4%). Transgenics showed a small but significant increase in bulk, but male transgenics had a lower staple strength than female transgenics and non-transgenics which did not differ significantly. There were no significant differences in fiber diameter, medullation, and hogget body weight. To our knowledge this is the first reported improvement in a production trait by genetic engineering of a farm animal without adverse effects on health or reproduction.
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: E, W* x+ F+ t0 a0 w# xhttp://www.nature.com/nbt/journal/v14/n2/pdf/nbt0296-185.pdf

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