科学家让工程细胞执行布尔逻辑门

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       据每日科学网5月31日（北京时间）报道，最近，美国约翰·霍普金斯大学通过基因工程对一种细胞进行改造，改造后的细胞就像表达“或（or）”和“与（and）”的布尔逻辑门，能根据一个或多个确定输入产生一个相应输出。研究人员指出，这一成果有望帮助科学家最终造出用细胞做微线路的计算机。相关论文发表在最近出版的《自然—化学生物学》上。  d) `: \- n/ `1 a) O7 O) l( z  e3 h
     Rapid and orthogonal logic gating with a gibberellin-induced dimerization system9 u7 E1 E% l  E0 p9 p! L
作 者：Takafumi Miyamoto,1, 5 Robert DeRose,1, 5 Allison Suarez,1 Tasuku Ueno,1 Melinda Chen,1 Tai-ping Sun,2 Michael J Wolfgang,3 Chandrani Mukherjee,4 David J Meyers4 & Takanari Inoue1
' g; @7 s/ s5 _期刊名称： nature chemical biology
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: @$ G2 [) n4 W       Using a newly synthesized gibberellin analog containing an acetoxymethyl group (GA3-AM) and its binding proteins, we developed an efficient chemically inducible dimerization (CID) system that is completely orthogonal to existing rapamycin-mediated protein dimerization. Combining the two systems should allow applications that have been difficult or impossible with only one CID system. By using both chemical inputs (rapamycin and GA3-AM), we designed and synthesized Boolean logic gates in living mammalian cells. These gates produced output signals such as fluorescence and membrane ruffling on a timescale of seconds, substantially faster than earlier intracellular logic gates. The use of two orthogonal dimerization systems in the same cell also allows for finer modulation of protein perturbations than is possible with a single dimerizer.
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