最新进展-Soft fibrin gels promote selection and growth of tumorigenic cells

饶冠华

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! ~! r0 q0 s$ K8 A8 L- rJing Liu, Youhua Tan, Huafeng Zhang, Yi Zhang, Pingwei Xu, Junwei Chen, Yeh-Chuin Poh, Ke Tang, Ning Wang & Bo Huang
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Nature Materials(2012)doi:10.1038/nmat3361Received 30 November 2011 Accepted 17 May 2012 Published online 01 July 2012 - }* O7 q: B. e- T! G& G! J  |' n

/ v! p% i- v* N$ YThe identification of stem-cell-like cancer cells through conventional methods that depend on stem cell markers is often unreliable. We developed a mechanical method for selecting tumorigenic cells by culturing single cancer cells in fibrin matrices of ~100 a in stiffness. When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days into individual round colonies that resembled embryonic stem cell colonies. Subcutaneous or intravenous injection of 10 or 100 fibrin-cultured cells in syngeneic or severe combined immunodeficiency mice led to the formation of solid tumours at the site of injection or at the distant lung organ much more efficiently than control cancer cells selected using conventional surface marker methods or cultured on conventional rigid dishes or on soft gels. Remarkably, as few as ten such cells were able to survive and form tumours in the lungs of wild-type non-syngeneic mice.
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利用这种新的方法能更快更方便地筛选出具有致瘤能力的细胞，大家有条件的话可以参考一下！（顺便求全文）6 P; `4 @4 @' U2 `$ N0 Y
http://www.nature.com/nmat/journ ... /full/nmat3361.html

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