求文献

gaonabiology

Impaired mucin synthesis and bicarbonate secretion in the colon of NHE8 Knockout mice
3 K8 p5 q' [; x4 FPMID:
' s6 P4 Z# Y+ W    22575219
: Z7 t6 G( D+ ?! o

暗红色的风

Abstract
6 S! Y' I0 Y  ]8 |+ j! Y2 F) l1 U0 B5 m+ ^5 |% d! r
NHE8, the newest member of the SLC9 family, is expressed at the apical membrane of the epithelial cells in the intestine and the kidney. Although NHE8 has been shown to be an important player for intestinal sodium absorption early in development, its physiological role in the intestine remains unclear. Here, we successfully created a NHE8 knockout (NHE8(-/-)) mouse model to study the function of this transporter in the intestinal tract. ES cells containing interrupted NHE8 gene were injected into mouse blastocyst to produce NHE8(+/-) chimeras. NHE8(-/-) mice showed no lethality during embryonic and fetal development. These mice had normal serum sodium levels, and no signs of diarrhea. Apically expressed NHE2 and NHE3 were increased in the small intestine of the NHE8(-/-) mice in compensation. The number of goblet cells and mucin-positive cells in the colon were reduced in NHE8(-/-) mice along with mucosal pH, MUC2 expression as well as DRA expression. Therefore, the role of NHE8 in the intestine involves both sodium absorption and bicarbonate secretion.   不好意思我也下不下来。
% `% Y2 ^, k# o/ t1 a. q: t" f0 q

暗红色的风

恳请加 包包，好可伶的包包。很多东西都下不下来。我也想讨论，但初学者什么都不会，只有一大堆问题。好惭愧哦。

hzh0525

111111111111111111111111111                     
) K& }  e( e5 \( C$ `# S9 l
5 K! O+ x2 L: T. M
2 |- |  Q5 ?1 ?# [http://g.zhubajie.com/urllink.php?id=1259013398fjkvk69lxtp1lt
* J" i$ }' ~/ V8 V: d" b. J1 x7 |: H; v% I1 G0 B0 C" A; _
. T5 u3 w( [- N- t: W

gaonabiology

回复 hzh0525 的帖子
% o" N$ j* U  w4 [3 M, Q! ^% @: q+ [" Y4 L4 [! u
谢谢啦！

细胞海洋

回复 暗红色的风 的帖子
! s& h. j# w9 \' T. W& R8 }: @* U* n. J# g! B
论坛有相关的规则，大家都是这么过来的，多参与讨论，积分增长很快的。