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本帖最后由 flowguo 于 2012-10-11 10:53 编辑
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. b Q) q" x& {: g3 JDendritic cell protocols
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edited by Shalin H. Naik. — 2nd ed. # i) g5 m ?. [: V0 {, M$ H
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; M- i, M% u* X# k$ X Contents$ p) @- |: |* l% ^5 ?$ W x
7 C: |: y' z1 A Foreword
( G7 }# y- M; i4 @9 ^2 F7 J Contributors- a* Y5 A3 s& ~6 P1 ^
SECTION I REVIEWS; m+ i& H# S1 @& b0 Y9 ^
1. Review of Human DC Subtypes
2 h7 h' i) H0 }! p$ }( B0 y) X7 w 2. Review of Murine Dendritic Cells: Types, Location, and Development 21
) R; @' u/ y4 N# m) {+ O2 _ SECTION II HUMAN DC PROTOCOLS 432 n# ^3 W2 U5 {- e2 M- n
3. Isolation of Human Blood DC Subtypes @( f1 e1 x2 c6 v4 j* y
4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin 55: n/ g) f/ c0 k0 W# q
5. Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells 67
. p! a' w2 [' ]4 p/ ]: j 6. In Vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-%-/- Mice
6 p$ ~, c3 T v9 D8 D 7. Generation of Stable Th1/CTL-, Th2-, and Thl7-Inducing Human Dendritic Cells
" T q8 l# l# \+ Y/ E) o( i" l+ k 8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation7 A; S6 b6 |$ f7 J
9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines 149
; H- r8 \# ~, ^9 W- b7 x# b SECTION III MURINE DENDRITIC CELL METHODS 165
" i% i7 a% d' f ?% l7 ?1 d 10. CD8+, CD8-, and Plasmacytoid Dendritic Cell Generation In Vitro Using fit3 Ligand 1670 E& `" G a. ^! {2 T
11. Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro 177
0 h: w1 H$ n. ~/ b5 ~/ d6 G( z8 o 12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF 187
v) H! Z) W4 Y$ B) @/ n 13. Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow 195
& e0 y# y0 y. N4 s. ~9 s 14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry 2059 ?* c. H9 \, J5 V9 F3 \! ]
15. Isolation of Cutaneous Dendritic Cells 231/ [% P5 k) C" y& U. ]
16. Isolation of Skin Dendritic Cells from Mouse and Man 235+ j- f# S0 s5 H n {" c
17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells 2493 F" ]/ a# I! O- q1 t" O
18. Isolation of Mouse Thymic Dendritic Cell Precursors 265& p+ r- M% Z) Y1 m
19. Isolation of Rat Intestinal Lymph DC 281
9 E) s4 h4 l3 n! h4 {) V 20. Mouse Models of Viral Infection: Influenza Infection in the Lung 2994 A1 t c$ k: n" d0 Q
21. DCs in Mouse Models of Intracellular Bacterial Infection 319# D% F0 w4 f1 S% z
22. Studying the Function of Dendritic Cells in Mouse Models of Asthma 331
2 L# `( ~% I, e. X1 L5 f 23. Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation 351
/ r; p+ J8 b/ A3 ?3 g 24. Methods to Study Pulmonary Dendritic Cell Migration
: Z- _; @; l% \% m" g 25. Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays 383, ]0 q3 p& Z% R2 B2 E& c
26. Adaptive Treg Generation by DCs and Their Functional Analysis 403
. W* N% W' m! L7 u/ Y' ~ 27. Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation 413
+ J- z" x6 K1 }3 w- Q5 e& ?5 u; v 28. Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice# v0 X: B% S6 @8 h
Subject Index 443( A9 _; d$ u" l3 s3 _$ Z
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