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; m t) z) B5 \, A. pPROTEIN PURIFICATION7 j% ` ^$ Q% b# k
Edited by Rizwan Ahmad4 ]# y+ m( p/ S$ U: }2 G' W
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Copyright © 2011 InTech) h$ P7 @% v' |7 Q( z
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Contents$ n, E6 Z, T0 e v* P& G, ^7 s
Preface VII
4 G# X- y. n8 _* hChapter 1 The Art of Protein Purification 1
- C8 P: }- C5 RWilliam Ward
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Chapter 2 The Isolation of Invertase from Baker’s Yeast –
8 k; @+ ], X6 BAn Introduction to Protein Purification Strategies 29
% s1 T. c6 J5 mAnthony P. Timerman6 o# |9 D( l1 C
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Chapter 3 Protein Purification by Affinity Chromatography 53 Luana C. B. B. Coelho, Andréa F. 1 n# r8 \; w$ [1 u3 J! E% W) j
S. Santos, Thiago H. Napoleão, Maria T. S. Correia and Patrícia M. G. Paiva
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4 s0 o* U& |" g# ~Chapter 4 Episomal Vectors for Rapid Expression
, ` n" n- m( ^! E: a0 Iand Purification of Proteins in Mammalian Cells 73! O! J0 y8 {9 d( }) N) X
Giovanni Magistrelli, Pauline Malinge, Greg Elson and Nicolas Fischer' v* |# S/ q$ q( N* @; i* R
' }' u* Y3 V8 i: g7 F2 Q" ?Chapter 5 Purification Systems Based on Bacterial Surface Proteins 89
! S, `" p& f4 x. BTove Boström, Johan Nilvebrant and Sophia Hober& |: F( P _# E8 H
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Chapter 6 The Denaturing and Renaturing are Critical Steps in the Purification7 K. I9 y1 Q+ @) O
of Recombinant Protein in Prokaryotic System 137
' p6 Y3 W G7 p5 {5 }1 C2 xDi Xiang, Yan Yu and Wei Han& D" ?' B6 N5 L
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Chapter 7 Identification and Characterization of
7 g! F) _& \1 I% bFeruloyl Esterases Produced by Probiotic Bacteria 151- @0 o7 f* {9 ~3 }+ N+ J4 {
Kin-Kwan Lai, Clara Vu, Ricardo B. Valladares, Anastasia H. Potts and Claudio F. Gonzalez8 U) \0 ]9 \2 @* R" }& e
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Chapter 8 Lectins: To Combat Infections 167
+ Z9 H3 N# Z. w6 |& v( [* x hBarira Islam and Asad U. Khan
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Chapter 9 Phosphoproteomics 189+ u; W6 t) E) U8 l# c9 ^
Francesco Lonardoni and Alessandra Maria Bossi
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