CFU,克隆形成率？

cartoonyang

在做ASC指标中，CFU （克隆形成率）的指标是很重要的，来这里请教下是如何做，如何染色？谢谢！

zerollx

回复 cartoonyang 的帖子: F9 @, i. A8 |

4 S/ I, Q( L0 J6 s3 E附件上传不了，请参考文献《Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: from small to large animal models》：
9 t9 ^5 S. l0 E: Z. N; i    The fibroblast-colony-forming unit (CFU-F) assay was performed as previously reported with minor modifications (Wu et al. 2000; Vidal et al. 2007). Starting from a suspension
3 w& Y1 p. D/ }* {7 i7 M- Econtaining 103 cells/ml, ASCs were plated by serial dilution in 6-well plates (from 48 cells/cm2 to 1 cell/cm2)and cultured in control medium supplemented with 20% FBS.At day 6, the medium was changed and, at day 10, cells were fixed with 100% methanol and stained with 0.5% crystal violet (Fluka, Buchs, Switzerland). The frequency of the CFU-F was established by scoring individual colonies (consisting of at least 20-30 cells) with respect to the number of seeded cells.