胎盘造血干流式有人做过么？

tinatina

所以应该进行正向和负向标记物同时进行流式标记，另外，CD34+细胞也不一定是HSC，组织中直接获得的未培养的MSC也有一定CD34表达，而HSC中也有一部分是CD34-性的。给您推荐一篇文章，摘要如下：
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$ C/ {8 w! t+ O7 S! P. pCytotherapy. 2012 Nov;14(10):1159-63. doi: 10.3109/14653249.2012.729817.$ Q5 M7 e4 |! t
Is CD34 truly a negative marker for mesenchymal stromal cells?
/ ?" s7 E' I$ l7 XLin CS, Ning H, Lin G, Lue TF." R5 n3 A; o2 a
Source
" T) O9 G  O7 [0 e4 N" s, o: oKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California , San Francisco, California 94143 – 0738, USA. clin@urology.ucsf.edu
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Abstract
: J4 b6 {& c0 e3 x) KThe prevailing school of thought is that mesenchymal stromal cells (MSC) do not express CD34, and this sets MSC apart from hematopoietic stem cells (HSC), which do express CD34. However, the evidence for MSC being CD34(-) is largely based on cultured MSC, not tissue-resident MSC, and the existence of CD34(-) HSC is in fact well documented. Furthermore, the Stro-1 antibody, which has been used extensively for the identification/isolation of MSC, was generated by using CD34(+) bone marrow cells as immunogen. Thus, neither MSC being CD34(-) nor HSC being CD34(+) is entirely correct. In particular, two studies that analyzed CD34 expression in uncultured human bone marrow nucleated cells found that MSC (BMSC) existed in the CD34(+) fraction. Several studies have also found that freshly isolated adipose-derived MSC (ADSC) express CD34. In addition, all of these ADSC studies and several other MSC studies have observed a disappearance of CD34 expression when the cells are propagated in culture. Thus the available evidence points to CD34 being expressed in tissue-resident MSC, and its negative finding being a consequence of cell culturing.

tinatina

CD34+ cell Tissue distribution, 人和鼠表达情况不一样哦+ G) M- L+ W9 e( W9 q
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Cells expressing CD34 (CD34+ cell) are normally found in the umbilical cord and bone marrow as hematopoietic cells, a subset of mesenchymal stem cells, endothelial progenitor cells, endothelial cells of blood vessels but not lymphatics (except pleural lymphatics), mast cells, a sub-population dendritic cells (which are factor XIIIa negative) in the interstitium and around the adnexa of dermis of skin, as well as cells in soft tissue tumors like DFSP, GIST, SFT, HPC, and in to some degree also in MPNSTs etc. It is important to mention that Long Term Hematopoietic Stem Cells [LT-HSCs] in mice and humans are the hematopoietic cells with the greatest self-renewal capacity. Human HSCs express CD34 marker but mice HSCs do not express CD34 marker.

incosc

人类造血干细胞的确定，目前最可信的方法是：移植进SCID动物（大鼠）体内后能够重建它们的造血系统的CD34+细胞。参看下面文字：
- U; k! R3 E% f; y) N) s/ h/ T* hThe human HSC/HPC system is not nearly as well defined as that of the mouse. Although human HSCs can be found in a population of CD34+ cells, HSCs are very rare in the CD34+ cell population. A highly purified CD34+ cell population is mainly composed of HPCs, and can include other cell types (for example, endothelial cells). Although there are in vitro assays that have in the past been used to identify HSCs, there are not many investigators at present who still consider in vitro assays as a definitive means to detect and evaluate the human HSC with long-term in vivo marrow repopulating capacity. The long-term culture-initiating cell or cobblestone area-forming cell may detect some cells of the human HSC population, but the system now considered to be the best indicator of identifying a functional human HSC is the in vivo SCID repopulating cell (SRC) assay.

fenglinzhu

之前有一同事跑过，好像是说CD34不高的来着，还是造血干不多来着的，具体方法不确定。

花饶然

回复 coffee.cat105 的帖子
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谢谢您的分享，能够进一步请教您么？您说的不断设门来取舍，具体是怎么操作？跨度是很大，该选那部分呢，各种疑惑啊？

花饶然

回复 tinatina 的帖子* o0 Q/ s; M7 ?2 C, e

4 f) [5 N0 A9 U是的，我也明白造血干也表达CD34-的，那么我到底该选择那些标记，什么方法会相对准确点？动物实验，我们也没有那个条件

凤城收藏

要确定是否造血干，采用半固体培养基做集落测试，做个判定，这是国际金标。

droface

应该不用

wf78365421

CD34+细胞也不一定是HSC，组织中直接获得的未培养的MSC也有一定CD34表达，而HSC中也有一部分是CD34-性的。

448922239

最近也在做这个胎盘造血干，请问楼主是不是直接从胎盘内消化下来的啊？？谢谢