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本帖最后由 细胞海洋 于 2014-1-4 09:43 编辑 ( S3 _- X+ m& @; n! N& d
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Molecular therapies
- c6 ~2 Z; a, ]; o. u& pDr. Zoltán Balajthy, Dr. János Aradi, Dr. Zoltán Balajthy, Dr. Éva Csősz, Dr. Beáta Scholtz, Dr. István Szatmári, Dr. József Tőzsér, Dr. Tamás Varga (2011)
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University of Debrecen7 P4 Z! W. H, o; @1 {& b
Copyright © 2011 The project is funded by the European Union and co-financed by the European Social Fund., Manuscript completed: 17 November 2011
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3 T- f' o3 O$ n+ U8 WIndex
$ r' t9 o( X, @1 j/ Y) D1. 1. Functional Genomics
" x+ e9 J! N0 B$ }7 ]" j1.1 Definitions
9 H/ ~6 y& D1 C9 X$ S' m1.2 About diseases0 `6 p/ b) R6 ] n9 ?; R- } ~2 S9 ?
1.3 Approaches to understanding disease mechanisms( F8 \4 [7 s* e
1.3.1 Gene expression is regulated in several basic ways
# t: k# P& a f; F" T8 W. y0 Z* r1.3.2 Microarrays: functional genomics in cancer research
8 ?# O: ?7 h. O m1 ^1.3.3 A Variety of Genetic Alterations Underlie Developmental Abnormalities and Disease) _7 U6 a0 R- A, P$ I- i7 H
1.3.4 Genomic microarrays
+ v" Z! e& c; \& T; |2. 2. Recombinant proteins
( Z p# r9 Z" D* s% M1 {2.1 Overview: Protein pharmaceuticals) {9 x1 R# G5 B, u: G
2.2 Cell-free systems: In vitro transcription and translation
+ w7 v3 O' l& z0 ^4 l2.3 Recombinant protein expression in isolated cells (cell culture)0 i" V' g: [" t) E4 k0 V( k
2.4 Non-prokaryotic expression systems
! q" q$ B4 ]$ y& q+ a( N2.4.1 Cloning in Pichia pastoris
, l0 L5 E& E8 ~7 s* {% ]$ L0 w" n2.4.2 Baculovirus mediated protein expression in insect cells
2 ?1 K# L* c+ s) g2.4.3 Mammalian expression systems
& B& ~0 I" r# }& k6 X3 T2.5 Purification of recombinant proteins* K$ U4 s, d R/ ^3 q
3. 3. Gene Therapy: Vectors and Strategies.* |; }) I, B+ }5 }( _2 w2 H- `/ E
3.1 Vectors for Gene Therapy
2 `' V' m1 V# C0 V/ _1 d) y3.2. Types of gene transfer, vectors for gene therapy& H7 V" C8 q. r- t1 R" h! V% D
3.3. General gene therapy strategies4 Y8 Z# E" [* I; A1 s; e) a
3.4. Human gene therapy. J$ O9 w. v) K' {1 u! z8 N h
4. 4. Protein replacement therapies.5 u% Y6 d: L. n& U% P/ ?: q
5. 5. Recombinant antibodies and the phage display technology9 }* k! b& x; I8 r# \7 U& A
5.1. Introduction
* i K, S5 Z1 Z; A, v5.1.1. The structure of antibodies and their production in the body) Q0 V3 L7 r' Q* ]6 v; G
5.1.2. Antigen-antibody binding
: T* B; b; c/ ?+ R! K5.2. The production of therapeutic antibodies
_9 p. j; B0 r+ |: H5.2.1. The production of antibodies in hybridoma cells.9 m% F7 X; [. B! _$ ?5 Q
5.2.2. Humanized antibodies
2 Q# W6 z2 _; ~+ J9 k5.2.3. Production of human antibodies
- o/ f* P2 p5 j6 f: Z8 e5.3. Generation of antibodies by phage display/ r- ]5 X' }' ]+ O: A
5.3.1. The phage display technology$ {: ^* H' J! w& Q5 l/ L1 b
5.3.2. Generation of phage libraries
( G7 ~5 G9 z' M5.4. Administration of therapeutic antibodies4 a/ y# A8 L% G2 T- x
6. 6. Anti-cytokine Therapy (sepsis).& e- b/ H# f. ^2 C. j
6.1. The consequences of developing inflammation
% s3 ^" G# A+ T4 S3 F; h6.2. Development of Inflammatory Response: Synthesis of Lipid Mediators
) @1 L' H* K! A4 ?6 M; ]6.3. Role of the Liver in Maintenance of Homeostasis: Acute Phase Response( w, J1 ?7 B0 ^: p
6.4. Time-Course of the Inflammatory Response During Sepsis
- j+ ]9 \, @( {0 @" Z7. 7. Animal models and transgenesis in biotechnology2 ~: K: h( C* P
8. 8. Embryonic and adult stem cells in regenerative medicine I. U8 B7 Y7 U( e. L1 p% i
8.1 Embryonic stem cells
, c) t& z7 Y/ Y: M" m8 f8.2 Somatic cell reprogramming into pluripotent stem cells5 @, T3 m; g# w( [4 Z
8.3 Adult stem cells
9 t; J$ w0 A E& G9. 9. Embryonic and adult stem cells in regenerative medicine II.9 c% G- {8 ? {! h$ p1 {
9.1 Pluripotent stem cells for regenerative medicine' d& k0 s) S l2 g
9.2 Clinical application of stem cells
. Y+ Q- K$ S! R, R7 g5 l9.3 Stem cell therapy to cure various diseases- B1 Y) p, B5 c/ T- Q
10. 10. Cell Cycle and Cancer Therapy, p53 I." f% i) s9 C, n. i$ C5 D1 E0 R" z
10.1 Cell Cycle! J7 r: u9 H1 b( P' O: k
10.2. Mitogenic Signaling in Eukaryotic Cell Controls the Rate of Cell Division' e3 q; c, Q/ c Y( o! B& a
10.3. Biochemical Events of Cell-cycle – in M Phase
2 h2 m, R; O3 ~0 h10.4. Protooncogenes
0 Y6 Q4 {6 V* n10.5. ErbB/HER Receptors
' c; R; U; I! j0 u$ t) l0 v0 B; r/ ?10.6. Therapeutic targets
" w/ H/ S# Q: O( o7 Y# E11. 11. Cell Cycle and Cancer Therapy, p53 II.
8 N1 |2 o# v3 j# ]11.1. Tumor Suppressor Genes and p539 z# r7 B( T0 S0 x0 k. f
11.2. Biochemical Pathways of Apoptosis and its Therapeutic Utilization
2 c9 x( l' h$ k1 J3 k7 n12. 12. Gene Silencing Technologies.! y$ s& ]; I9 e" O0 p4 D- I
12.1 Introduction6 u. d8 @8 O9 i& L( r& O! ~
12.2 Action of antisense oligonucleotides/ l* p+ f5 |* F4 l1 {
12.3 Chemical modifications of gene silencing oligonucleotides; general considerations1 _' g6 U* b* \" W8 {6 F3 r9 Q
12.4 Inhibition of transcription by triple helix forming oligonucleotides
8 H( E! A( x: h( \3 K1 g12.5 Gene silencing by ribozymes
! k, J+ R; L3 B' U; q% ~12.6 Gene silencing with short RNA fragments
' ]6 a8 [) E" q' i12.7 Important final note
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发表于 2014-1-3 16:18
