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Finally, Mabuchi himself had isolated myosin from the cortex of dividing sea urchin and starfish eggs (Mabuchi, 1973; Mabuchi, 1974). From all of this, the University of Tokyo duo concluded, "it would be reasonable to suspect that actin and myosin would interact to produce the force of constriction." To test this suspicion, Mabuchi says he decided the microinjection of myosin antibodies would be "the only promising method." He injected an antibody raised against starfish egg myosin into dividing eggs in the first experiment to use antibodies as protein inhibitors in live cells.$ K# T# ^! X3 f# ^
" N& O8 j! w2 V% b% O2 [5 LCytokinesis fails after injection of anti-myosin antibodies.6 X7 s( ?3 r7 E- ]
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, x: F& \5 H6 RThe antibody inhibited the actin-activated ATPase activity of myosin in a dose-dependent manner. And when it was injected into eggs during the second interphase, it stopped all subsequent cleavage events. Mitosis still occurred normally in most of the injected cells, even if cleavage was inhibited. The study solidified a role for actin–myosin contraction in cytokinesis but not nuclear division〞and, equally important, a role for antibodies in disrupting cellular phenomena.
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Fujiwara, K., and T.D. Pollard. 1976. J. Cell Biol. 71:848–875.
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Mabuchi, I. 1973. J. Cell Biol. 59:542–547.8 U2 A# M6 I5 y9 S( z- u
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Mabuchi, I. 1974. J. Biochem. 76:47–55.& |& e) ]# u4 @
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Mabuchi, I., and M. Okuno. 1977. J. Cell Biol. 74:251–263.
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" E( j! j$ X! A4 v, o/ B; uRappaport, R. 1967. Science. 156:1241–1243.(The stage was set in 1977 for Issei Mabu) |
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发表于 2009-3-6 08:55
发表于 2015-6-18 13:44
