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After harvesting a test tube of eggs from Rana pipiens frogs, Lohka could spin the eggs at low speed and pop the cytoplasmic contents out of their plasma membranes "just like taking the skin off a grape." If he added Xenopus laevis sperm heads to the activated cytoplasm, he observed that the sperm heads transformed into pronuclei and then mitotic chromosomes (Lohka and Masui, 1983).
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& O8 n1 x! Q! r& `2 MBut it wasn't until Lohka and advisor Yoshio Masui looked at their extract-plus-sperm preparations by electron microscopy that they realized that not only did the sperm nucleus decondense, but a nuclear envelope was assembling around it as well (Lohka and Masui, 1984). Lohka provided one of the first descriptions of envelope assembly: membrane vesicles flattened and fused into a double-membraned structure, complete with nuclear pores. In addition, if Lohka fractionated the egg extracts with a higher spin and separated them into soluble and particulate fractions, he could show that envelope assembly required both.. S [5 c4 ?2 {
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Lohka credits Masui's love of unorthodox approaches for the discovery that egg cytoplasm can support cellular activities at least for short periods. "That was back when how you did experiments was an expression of your personality," says Lohka, now at the University of Calgary in Canada.
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9 w2 V4 A$ S( T$ Y$ c; VFrog egg extracts allow the study of nuclear envelope formation.
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He notes that the study outcomes were not as important in the long run "as the notion that you could actually get quite complex cell processes to occur in cell-free extracts." The system was used to purify metaphase-promoting factor (Lohka et al., 1988), which led to the identification of cdc2 and cyclin as its components (Gautier et al., 1990) and the extracts became a valuable tool for further investigating the cell cycle (Murray, 1991). The system has also been used to study nuclear transport (Newmeyer et al., 1986), DNA replication (Mills et al., 1989), and spindle microtubule dynamics (Heald et al., 1996).) w+ Q( I( \- Q% \& b7 W
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Gautier, J., et al. 1990. Cell. 60:487–494.
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Heald, R., et al. 1996. Nature. 382:420–425.
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# s0 j, l3 }4 n1 ^4 HLohka, M.J., and Y. Masui. 1983. Science. 220:719–721.
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; h) M$ ]2 o \8 Z& A* K" [Lohka, M.J., and Y. Masui. 1984. J. Cell Biol. 98:1222–1230.- v$ K# R% }8 y U+ H
: H- ]4 N% @4 y) o' ULohka, M.J., et al. 1988. Proc. Natl. Acad. Sci. USA. 85:3009–3013.
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Mills, A.D., et al. 1989. J. Cell Sci. 94:471–477., x5 V3 J/ B) \( h" y
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Murray, A.W. 1991. Methods Cell Biol. 36:573–597.
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Newmeyer, D.D., et al. 1986. J. Cell Biol. 103:2091–2102.(For his graduate project at the Universi) |
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发表于 2009-3-6 08:56
发表于 2015-5-29 15:09
