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1 Department of Medicine, The Sackler Institute, University College London, London WC1E 6JJ, England, UK( V# k- i6 m2 P* N: [! [
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2 Department of Biochemistry and Molecular Biology, University College London, London WC1E 6BT, England, UK1 }% D+ r6 A, h
8 V6 o0 G4 ^/ u# r5 Y) K" `8 v, Q3 School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, England, UK
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7 R! A% p4 g8 oCorrespondence to Marco Falasca: m.falasca@ucl.ac.uk4 \* [2 ~, y+ ^7 t) z- o
* y8 t1 Y) A6 t6 d0 P2 B% JThe lipid products of phosphoinositide 3-kinase (PI3K) are involved in many cellular responses such as proliferation, migration, and survival. Disregulation of PI3K-activated pathways is implicated in different diseases including cancer and diabetes. Among the three classes of PI3Ks, class I is the best characterized, whereas class II has received increasing attention only recently and the precise role of these isoforms is unclear. Similarly, the role of phosphatidylinositol-3-phosphate (PtdIns-3-P) as an intracellular second messenger is only just beginning to be appreciated. Here, we show that lysophosphatidic acid (LPA) stimulates the production of PtdIns-3-P through activation of a class II PI3K (PI3K-C2?). Both PtdIns-3-P and PI3K-C2? are involved in LPA-mediated cell migration. This study is the first identification of PtdIns-3-P and PI3K-C2? as downstream effectors in LPA signaling and demonstration of an intracellular role for a class II PI3K. Defining this novel PI3K-C2?–PtdIns-3-P signaling pathway may help clarify the process of cell migration and may shed new light on PI3K-mediated intracellular events.$ Y2 ~ M: O2 _ Z7 S# Y
% U& v! o4 A. C6 x% L3 OAbbreviations used in this paper: LPA, lysophosphatidic acid; PI3K, phosphoinositide 3-kinase; PLC, phospholipase C; PtdIns-3,4,5-P3, phosphatidylinositol-3,4,5-trisphosphate; PtdIns-3-P, phosphatidylinositol-3-phosphate.
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! S$ X+ _! q/ F' Y, O1 H7 CIntroduction
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) m7 C+ m) {6 S6 b0 T1 A& K9 mOver the last few years, the intracellular role of phosphoinositide 3-kinases (PI3Ks), the family of enzymes responsible for generation of 3-phosphorylated phosphoinositides, has been extensively investigated and it is now established that PI3Ks are crucial components of many signaling pathways playing a pivotal role in many different physiological events (Rameh and Cantley, 1999; Cantley, 2002). Furthermore, it is also well documented that altered PI3K-dependent pathways are implicated in different diseases including cancer and diabetes (Katso et al., 2001). Although different PI3Ks have been identified and grouped into three classes (Foster et al., 2003), the majority of these studies focused on members of class I and their main in vivo product phosphatidylinositol-3,4,5-trisphosphate (PtdIns-3,4,5-P3).
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* j5 ~ X2 M/ |Recently, a growing interest has arisen in the members of class II PI3K and several lines of evidence suggest a potential role for these enzymes in agonist-mediated regulation of cellular functions (Foster et al., 2003). Such evidence includes activation of PI3K-C2 by insulin (Brown et al., 1999) and monocyte chemotactic peptide-1 (Turner et al., 1998) and through interaction with clathrin (Gaidarov et al., 2001). Similarly, insulin (Brown and Shepherd, 2001) and platelet aggregation (Zhang et al., 1998) have been reported to activate PI3K-C2?. In addition, PI3K-C2 and PI3K-C2? associate with polypeptide growth factor receptors (Arcaro et al., 2000) and recent data suggest that PI3K-C2? is involved in EGF- and stem cell factor–dependent signals (Arcaro et al., 2002). Despite this evidence, a clear mechanism of activation and the precise intracellular roles of these enzymes are still not defined (Foster et al., 2003). Furthermore, there is currently no clear indication of their in vivo lipid products (Foster et al., 2003), although the observation that, in vitro, these enzymes display a strong substrate preference for phosphatidylinositol suggests phosphatidylinositol-3-phosphate (PtdIns-3-P) to be their main lipid product in vivo (Brown and Shepherd, 2001).
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; V+ w4 z9 d7 O4 _The role of PtdIns-3-P as a dynamic intracellular second messenger has been recently underscored by our work reporting that this phosphoinositide is generated upon insulin stimulation (Maffucci et al., 2003) and the demonstration that it plays a crucial role in insulin signaling (Chaussade et al., 2003; Maffucci et al., 2003). Evidence suggests that the insulin-dependent pool of PtdIns-3-P might be generated through activation of a class II PI3K enzyme (Maffucci et al., 2003).5 k& r7 x0 }7 W0 B, _
3 D2 ^0 n$ N2 k3 ]Lysophosphatidic acid (LPA; 1-acyl-sn-glycerol-3-phosphate) is a serum-borne phospholipid that evokes a wide range of biological effects, including cell proliferation, migration, and survival (Moolenaar, 1994; Graler and Goetzl, 2002; Mills and Moolenaar, 2003; van Leeuwen et al., 2003). This large spectrum of biological activities is explained by the fact that LPA receptors can couple to three distinct G proteins, Gq, Gi, and G12/13, leading to activation of several intracellular messengers, including phospholipase C (PLC), the small GTPases RhoA and Rac, the Ras–MAPK cascade, and PI3K (Radeff-Huang et al., 2004). Two members of class I PI3K (p110? and p110) have been reported to be involved in LPA signaling (Yart et al., 2002). In particular, p110 can be directly activated by G protein ? subunits through association with the adaptor protein p101 (Stephens et al., 1997) and it has been implicated in the LPA-dependent Ras–MAPK activation (Takeda et al., 1999) and motility (Jung et al., 2004). Furthermore, it has been shown that at least in some cell lines the LPA-dependent accumulation of PI3K products is mediated by p110? in a mechanism involving transactivation of the EGF receptor (Laffargue et al., 1999).
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% p# ]: _* g! KAmong the different effects elicited by LPA, migration has recently received increasing attention (Gschwind et al., 2003; Stahle et al., 2003; van Leeuwen et al., 2003; Yamada et al., 2004). The importance of LPA in migration has been underscored by the discovery that autotaxin, a cell motility-stimulating ectophosphodiesterase involved in tumor invasion, can act as a lysophospholipase D and generate LPA from lysophosphatidylcholine in the extracellular environment (Umezu-Goto et al., 2002). Indeed, both LPA and autotaxin have been involved in motility of neoplastic and nonneoplastic cells (Hama et al., 2004). Cell migration is the result of different and well-coordinated physical processes and orchestrates embryonic morphogenesis, contributes to tissue repair and regeneration, and drives disease progression in cancer, atherosclerosis, and arthritis. Several lines of evidence suggest that 3-phosphoinositides play a role in directed migration via both G protein– and tyrosine kinase–mediated signaling processes. Here, we identify a novel pathway in LPA signaling that involves generation of the lipid second messenger PtdIns-3-P through activation of PI3K-C2? and is crucial for LPA-dependent cell migration. This study is the first demonstration of an intracellular role for a class II PI3K and identification of two new important players in cell migration.
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Results
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2 S3 [) N+ v: kLPA generates the intracellular second messenger PtdIns-3-P
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# ~9 a2 ?* |+ P4 V6 F& b) oWe have recently demonstrated for the first time that PtdIns-3-P can act as a lipid second messenger and plays a crucial role in insulin signaling (Maffucci et al., 2003). This observation, together with our previous work reporting that LPA can generate PtdIns-3-P in COS7 cells (Razzini et al., 2000), prompted us to further investigate the effects of LPA stimulation on the levels of the PI3K products in different cell lines. HPLC analysis revealed that LPA stimulated the production of PtdIns-3-P in 3H-labeled COS7 (Fig. S1, A and B, available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1), HeLa cells (Fig. 1 A), and an ovarian cancer cell line (SKOV-3; Fig. 1 B). It is important to underline that resting mammalian cells contain high basal levels of PtdIns-3-P (Rameh and Cantley, 1999); therefore, the apparent small increase upon LPA stimulation actually corresponds to a large amount of this phosphoinositide being generated de novo.
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& H& F$ {) t) _Figure 1. LPA generates specifically PtdIns-3-P in HeLa and SKOV-3. HeLa (A) and SKOV-3 (B) were labeled with -myo-inositol for 24 h and stimulated with 25 μM LPA for the indicated times. Phospholipids were then extracted, deacylated, and analyzed by HPLC. Levels of PtdIns-3-P are shown as a percentage of basal levels. Data are mean ± SEM of three to four (HeLa) and three to eight (SKOV-3) independent experiments. (C) Serum-starved HeLa were labeled with phosphoric acid for 3 h and stimulated with 25 μM LPA for the indicated times. Phospholipids were then extracted and analyzed by TLC. (D) Levels of PtdIns-3,4,5-P3 in 3H-labeled HeLa assessed at different times of EGF stimulation by HPLC analysis. Data are mean ± SEM of three independent experiments. (E) Western blotting analysis of lysates from serum-starved HeLa stimulated with 25 μM LPA for the indicated times. Phosphorylation of Akt on residue Ser473 and Thr308 was assessed by using specific antibodies. Filters were stripped and reprobed with an anti-Akt antibody. (F) Western blotting analysis of lysates from serum-starved SKOV-3 stimulated with 25 μM LPA for the indicated times. Phosphorylation of Akt and total Akt levels were assessed by using specific antibodies. (G) LPA-dependent activation of PLC was assessed by monitoring the levels of total inositol phosphates generated. Data are from the experiment performed in parallel with the experiment shown in F. (H) Western blotting analysis of lysates from serum-starved SKOV-3 stimulated with 20 ng/ml EGF for the indicated times. Phosphorylation of Akt and total Akt levels were assessed by using specific antibodies.
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When we analyzed PtdIns-3,4,5-P3, we observed that the levels of this phosphoinositide were very low in resting COS7 but rapidly increased upon LPA stimulation (Fig. S1 C), as reported previously (Laffargue et al., 1999). In contrast, HPLC analysis of 3H-labeled cells revealed that both SKOV-3 (Fig. S2 A, available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1) and HeLa (unpublished data) possessed high basal levels of PtdIns-3,4,5-P3 that were unaffected by LPA stimulation. As an alternative method to detect and visualize PtdIns-3,4,5-P3, phospholipids were extracted from 32P-labeled cells and analyzed by TLC. Stimulation with LPA for different times did not increase the basal levels of PtdIns-3,4,5-P3 in HeLa (Fig. 1 C). However, a clear increase in PtdIns-3,4,5-P3 was observed in HeLa upon EGF stimulation in experiments performed in parallel (HPLC analysis of 3H-labeled cells: Fig. 1 D; TLC analysis of 32P-labeled cells: see Fig. 3 C). We checked the phosphorylation state of the well-established PtdIns-3,4,5-P3 target protein kinase B/Akt. As a consequence of the observed high basal levels of PtdIns-3,4,5-P3, phosphorylation of Akt was already detectable in unstimulated HeLa (Fig. 1 E) and SKOV-3 (Fig. 1, F and H). LPA stimulation for different times did not induce a further phosphorylation of Akt in either cell line (Fig. 1, E and F). Efficiency of LPA in stimulating these cells was confirmed by monitoring PLC activation in parallel experiments (Fig. 1 G, representative example of PLC activation in SKOV-3 in the experiment performed in parallel to the experiment shown in Fig. 1 F). The possibility that Akt was already maximally activated in these cells and therefore could not be further stimulated was ruled out by the observation that EGF induced a clear increase in phosphorylation of Akt in SKOV-3 (Fig. 1 H) as previously reported (Anderson et al., 2001). Similar results were obtained in HeLa (unpublished data). Together, these results indicate that LPA specifically stimulates PtdIns-3-P synthesis without detectably affecting the levels of PtdIns-3,4,5-P3 at least in HeLa and SKOV-3.8 d% R' k: y$ \
& o/ C. u( f0 b* VThe LPA-dependent pool of PtdIns-3-P is generated at the plasma membrane& w. m) n# x5 x2 Z: N% G
: ~# T! e+ ]: a" j) ]To localize the LPA-dependent pool of PtdIns-3-P, cells were transfected with a cDNA encoding GFP-2XFYVEHrs, a GFP fusion protein known to specifically bind PtdIns-3-P (Gillooly et al., 2000; Maffucci et al., 2003). Confocal microscopy analysis revealed that in COS7 (Fig. S1 D), HeLa (Fig. 2 A), and SKOV-3 (Fig. 2 B), LPA stimulation induced a rapid translocation of GFP-2XFYVEHrs to the plasma membrane that paralleled the time course of PtdIns-3-P formation observed by HPLC analysis (for COS7 compare Fig. S1 B and histogram in Fig. S1 D; for SKOV-3 compare Fig. 1 B and Fig. S2 B). As shown in Fig. 2 C, plasma membrane translocation in SKOV-3 was confirmed by colocalization of YFP-2XFYVEHrs and the plasma membrane marker CFP-PH PLC1 (Maffucci et al., 2003).1 n$ X' D! z# C
) u7 W2 b% X' ]! j% ?8 H0 CFigure 2. The LPA-dependent pool of PtdIns-3-P is generated at the plasma membrane of HeLa and SKOV-3. HeLa (A) and SKOV-3 (B) were transfected with a cDNA encoding GFP-2XFYVEHrs. After 24 h, cells were serum deprived and stimulated with 25 μM LPA for the indicated times before fixing for confocal microscopy analysis. (C) Confocal microscopy analysis of SKOV-3 transfected with cDNAs encoding YFP-2XFYVEHrs and CFP-PH PLC1 and stimulated with 25 μM LPA for 10 min. Bars, 10 μm.
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1 T- M0 V) Z* y YTo investigate the involvement of PI3K in generation of the LPA-dependent pool of PtdIns-3-P, we performed HPLC analysis in 3H-labeled SKOV-3 pretreated with different concentrations of the reversible PI3K inhibitor LY294002 before stimulation with LPA. Pretreatment with LY294002 up to 10 μM did not inhibit the LPA-mediated formation of PtdIns-3-P (Fig. 3 A), although it efficiently reduced the basal levels of PtdIns-3,4,5-P3 (unpublished data) and inhibited the basal phosphorylation of Akt (Fig. 3 B). Furthermore, TLC analysis revealed that 10 μM LY294002 completely blocked the EGF-induced generation of PtdIns-3,4,5-P3 in SKOV-3 and HeLa (Fig. 3 C). Similarly, pretreatment with 100 nM of the irreversible PI3K inhibitor wortmannin inhibited the LPA-induced translocation of the PtdIns-3-P fluorescent probe GFP-2XFYVEHrs to the plasma membrane, whereas 10 μM LY294002 had almost no effect (Fig. 3, D and E). These data indicate that LPA generates PtdIns-3-P at the plasma membrane of COS7, HeLa, and SKOV-3 in a mechanism sensitive to wortmannin but, unlike agonist-induced PtdIns-3,4,5-P3 production, more resistant to LY294002.- t8 Z8 q& @) _$ w
% b! t0 |8 f4 Y6 [, s) lFigure 3. Effect of PI3K inhibitors on the LPA-mediated PtdIns-3-P formation. (A) SKOV-3 were labeled with -myo-inositol for 24 h and left untreated or pretreated with the indicated concentrations of LY294002 before stimulation with 25 μM LPA for 10 min. Phospholipids were then extracted, deacylated, and analyzed by HPLC. Levels of PtdIns-3-P are shown as a percentage of basal levels. (B) Western blotting analysis of lysates from serum-starved SKOV-3 treated with 100 nM wortmannin or 10 μM LY294002 for 30 min. Phosphorylation of Akt on residue Ser473 was assessed by using a specific antibody. Filter was stripped and reprobed with an anti-Akt antibody. (C) SKOV-3 (top) and HeLa (bottom) were labeled with phosphoric acid for 3 h and left untreated or pretreated with 10 μM LY294002 before stimulation with 20 ng/ml EGF for 5 (SKOV-3) or 1 min (HeLa). Phospholipids were extracted and analyzed by TLC. (D) GFP-2XFYVEHrs-expressing SKOV-3 cells were pretreated with 100 nM wortmannin or 10 μM LY294002. After 30 min, cells were stimulated with 25 μM LPA for 10 min before fixing for confocal microscopy analysis. Bar, 10 μm. (E) Quantitative analysis of experiments as in D. Data are mean ± SEM of five independent experiments.& c: O% v5 |. v2 m9 l( `9 w+ [
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The LPA-dependent pool of PtdIns-3-P is involved in cell migration* @! D* G7 r+ h+ @5 u6 i6 A3 r
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To gain further insight into the LPA signaling in HeLa and SKOV-3 and precisely assess the role of the LPA-stimulated pool of PtdIns-3-P, we tested the effects of LPA on cell proliferation and migration in both cell lines. LPA had only a small effect on cell proliferation in HeLa, whereas it clearly increased cell growth in SKOV-3 (Fig. S3 A, available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1). In contrast, LPA induced migration in both cell lines as assessed by wound healing (Fig. 4 A; Fig. 5 A; and Fig. S3, B and C) and Transwell (Fig. 4 C, Fig. 5 C, and Fig. S3 D) assays. Therefore, we hypothesized that PtdIns-3-P might be involved in the LPA-mediated cell motility.
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Figure 4. Effect of wortmannin on the LPA-mediated migration. (A and B) Time-lapse microscopy of wound healing assay in SKOV-3 pretreated with the indicated concentrations of wortmannin for 30 min. Images in A show the wounded cell monolayers at time 0 and after the indicated hours in the absence (control) or presence of 25 μM LPA with or without the inhibitor. (B) Quantitative analysis was performed as described in Materials and methods. Data are mean ± SEM of three to four independent experiments. (C) Serum-starved SKOV-3 were pretreated with the indicated concentrations of wortmannin for 30 min. Migration was assessed by Transwell assay in the presence of 25 μM LPA and the indicated concentrations of the inhibitor. Data are mean ± SEM of five independent experiments and are expressed as a percentage of LPA-stimulated cells. In these experiments, LPA induced a three- to fourfold increase in cell migration.
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Figure 5. Effect of LY294002 on the LPA-mediated migration. (A and B) Time-lapse microscopy of wound healing assay in SKOV-3 pretreated with the indicated concentrations of LY294002 for 30 min. Images in A show the wounded cell monolayers at time 0 and after the indicated hours in the absence (control) or presence of 25 μM LPA with or without the inhibitor. (B) Quantitative analysis was performed as described in Materials and methods. Data are mean ± SEM of three to five independent experiments. (C) Serum-starved SKOV-3 were pretreated with the indicated concentrations of LY294002 for 30 min. Migration was assessed by Transwell assay in the presence of 25 μM LPA and the indicated concentrations of the inhibitor. Data are mean ± SEM of five independent experiments and are expressed as a percentage of LPA-stimulated cells. In these experiments, LPA induced a three- to fourfold increase in cell migration.
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; g+ D$ D1 H5 s8 S! T" b7 eEffect of PI3K inhibitors.
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3 b4 p9 B. [5 i! J3 eTo check this hypothesis, we first tested the effect of different concentrations of PI3K inhibitors on cell migration. Wound healing assay monitored by time-lapse microscopy (Fig. 4, A and B) and Transwell assay (Fig. 4 C) indicated that a pretreatment with wortmannin inhibited the LPA-dependent migration. In contrast, a concentration of 1 μM LY294002 did not affect the LPA-dependent migration (Fig. 5, A and B) and 10 μM LY294002 had only a partial inhibitory effect (Fig. 5, A and B, wound healing assay monitored by time-lapse microscopy; Fig. 5 C, Transwell assay). Together, these data indicate that generation of the LPA-dependent pool of PtdIns-3-P and the LPA-induced migration show a similar sensitivity to PI3K inhibitors.2 k; Z+ `) V, @+ W9 k
n I4 E% O1 | I* ?. ~* v- K" TEffect of PtdIns-3-P–specific phosphatase.5 w' @) l) x; {% ~3 l; d3 S o
1 D7 e$ X" d5 X" H% u* Y. kThese observations further prompted us to specifically check a potential role for PtdIns-3-P in LPA-dependent migration. Therefore, we analyzed migration of SKOV-3 transfected with a cDNA encoding a Flag-tagged PtdIns-3-P–specific phosphatase, myotubularin MTM1 (Laporte et al., 2002), that has been recently successfully used to inhibit the insulin-dependent pool of PtdIns-3-P (Chaussade et al., 2003). In these experiments, migration was assessed in Transwell assays by counting the total number of migrated cells as described in Materials and methods. Although the transfection efficiency in SKOV-3 was only 50%, overexpression of MTM1 was able to reduce the LPA-induced migration whereas overexpression of the point mutant MTM1 C375S, which has no phosphatase activity toward PtdIns-3-P, had no effect (Fig. 6 A, top). Expression of both proteins was assessed in Western blotting analysis (Fig. 6 A, bottom). Although expression of MTM1 was able to induce a partial inhibition of migration, it did not seem to affect the levels of PtdIns-3,4,5-P3 as basal Akt phosphorylation was not even partially reduced (Fig. 6 B). Importantly, the lack of effect on Akt phosphorylation was not due to the transfection efficiency because overexpression of the PtdIns-3,4,5-P3–specific phosphatase PTEN at similar transfection efficiency was indeed able to reduce Akt phosphorylation (Fig. 6 C).
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Figure 6. PtdIns-3-P is involved in the LPA-mediated migration of SKOV-3. (A-G) SKOV-3 were transiently transfected with the indicated cDNAs. Transfection efficiency in these cells was 50%. (A, top) SKOV-3 were transfected with cDNAs encoding Flag-MTM1 and Flag-MTM1 C375S. After 24 h, cells were serum deprived overnight and migration was assessed by Transwell assay in the presence of 25 μM LPA. The total number of migrated cells was counted after staining the membranes of the Transwell chambers with crystal violet. Data are mean ± SEM of three independent experiments. *, P 80%). After 24 h, cells were serum deprived overnight and wound healing was performed in the presence of 25 μM LPA. (H) Representative phase-contrast images of the wounded cell monolayers at time 0 and after 24 h of migration. (I) Quantitative analysis of wound healing as in H. Data are mean ± SEM of three independent experiments.* h5 _- D! J" \3 C/ F0 q, _3 p
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Effect of PtdIns-3-P–specific binding domains.
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We analyzed the LPA-dependent migration in cells overexpressing the GFP-2XFYVEHrs fusion protein that we hypothesized might act as a PtdIns-3-P–sequestering domain and therefore interfere with the LPA-dependent pool of PtdIns-3-P. Wound healing assays monitored by confocal microscopy revealed that overexpression of GFP-2XFYVEHrs inhibited the LPA-dependent migration in SKOV-3, whereas overexpression of GFP alone had no effect (Fig. 6 D). Because none of the GFP-2XFYVEHrs-transfected cells were able to migrate into the wounded area, we could not perform a quantitative analysis of this assay (Fig. 6 D). Therefore, to quantify the effect of overexpression of the PtdIns-3-P–binding domain, we transfected cells with cDNAs encoding GFP alone or GFP-2XFYVEHrs and analyzed migration by using Transwell assays. In a first set of experiments, we counted the total number of cells migrated after staining the membranes of the Transwell chambers with crystal violet and we observed that LPA clearly stimulated migration in GFP-transfected cells, whereas overexpression of GFP-2XFYVEHrs partially reduced the LPA-dependent migration (Fig. 6 E). The observed partial effect was consistent with a transfection efficiency of 50%, as discussed in the previous paragraph./ A7 A4 l1 @; Q/ x0 g# y! x+ f/ V
% w3 q8 ?* Z! t% |* K- w3 jTo clearly assess the effect of the PtdIns-3-P–sequestering domain, we performed a very accurate second set of experiments and counted only the transfected (green) cells that had migrated. Indeed, in this more precise assay we observed that migration was completely inhibited in cells expressing GFP-2XFYVEHrs, whereas cells expressing GFP alone migrated upon LPA stimulation (Fig. 6 F). As discussed in the previous section, although a 50% transfection efficiency of GFP-PTEN was enough to reduce Akt phosphorylation, basal levels of Akt phosphorylation were not even partially reduced in cells transfected with GFP-2XFYVEHrs with the same efficiency (Fig. 6 G).
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When we performed similar experiments in HeLa we observed a total inhibition of the LPA-mediated migration in cells expressing GFP-2XFYVEHrs, a finding consistent with the high transfection efficiency of these cells (>80%; Fig. 6, H and I; and Fig. S3 E). To further confirm that inhibition of migration was due to a specific sequestering of PtdIns-3-P, we transfected HeLa with a cDNA encoding another fusion protein also known to bind PtdIns-3-P (GFP-PX p40phox) that we have already used to monitor the intracellular PtdIns-3-P (Maffucci et al., 2003). As a control we used a mutant of this domain with a reduced ability to bind PtdIns-3-P (GFP-PX p40phox Y59A/L65Q). GFP-PX p40phox inhibited the LPA-mediated migration, whereas less effect was observed in cells transfected with the mutated version of the protein (Fig. 6, H and I). Similar results were obtained in Transwell assay (Fig. S3 E). Overexpression of these domains did not affect cell growth (Fig. S3 F).
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; `/ W( p- f7 u3 iTogether these data indicate that the lipid second messenger PtdIns-3-P is a new effector in LPA cascade and plays a role in the LPA-mediated cell migration.
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5 K% q# {' P0 g7 I+ G. |" c0 V) f, xThe LPA-dependent pool of PtdIns-3-P is generated through PI3K-C2? activation
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The observation that in HeLa and SKOV-3 LPA did not affect the levels of PtdIns-3,4,5-P3, the major in vivo product of class I PI3Ks, together with the observed resistance to the inhibitor LY294002 ruled out the possibility that PtdIns-3-P derives from activation of the class I PI3Ks. This finding is in agreement with recent data indicating that the LPA-dependent migration of SKOV-3 requires activation of a Ras–MEK kinase 1 pathway but not class I PI3K (Bian et al., 2004). Several lines of evidence suggest that, at least in insulin signaling, the stimulated pool of PtdIns-3-P is the product of a class II PI3K (Maffucci et al., 2003). Moreover, the high sensitivity to wortmannin and reduced sensitivity to LY294002 observed in this study was in agreement with observed in vitro sensitivities of the class II enzyme PI3K-C2? to these inhibitors and is distinct from that observed for class I enzymes (Arcaro et al., 1998; Fig. S4, A and B, available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1). On the basis of these observations, we hypothesized that the LPA-dependent pool of PtdIns-3-P might be generated through activation of a class II PI3K.5 x$ ~" e7 G3 _$ f! S b- ^7 X
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To investigate this hypothesis, we examined the effect of specific inhibition of these isoforms on the LPA-dependent generation of PtdIns-3-P at the plasma membrane. As there are no class II PI3K-specific chemical inhibitors available, we used RNA interference to specifically knockdown the class II isoforms PI3K-C2? and PI3K-C2. Then, we analyzed the intracellular localization of GFP-2XFYVEHrs in LPA-stimulated SKOV-3 transfected with the corresponding siRNAs. A complete inhibition of LPA-dependent GFP-2XFYVEHrs plasma membrane translocation was observed in SKOV-3 transfected with the PI3K-C2? siRNA. No inhibition was observed in cells transfected with the PI3K-C2 siRNA (Fig. 7, A and B). The observed complete inhibition was consistent with a high uptake of siRNAs in SKOV-3 that was confirmed by Western blotting analysis showing that a >90% knockdown of protein expression was achieved (Fig. 7 C). The higher transfection efficiency compared with plasmid DNAs is in agreement with reported high uptake of siRNAs in cells (Holen et al., 2002; Splinter et al., 2003). The LPA-dependent activation of PI3K-C2? was confirmed by in vitro lipid kinase assay (Fig. 8 A). In the same experiments, no activation of PI3K-C2 was observed (unpublished data). In addition, we observed that LPA induced the translocation of a myc-tagged version of PI3K-C2? to the plasma membrane in both HeLa (Fig. 8 B) and SKOV-3 (Fig. 8 C), suggesting that formation of PtdIns-3-P might occur through an LPA-dependent targeting and subsequent activation of the enzyme at the plasma membrane.( K; f5 Z! O @- a5 z
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Figure 7. Knockdown of PI3K-C2? completely inhibits the LPA-mediated PtdIns-3-P formation. (A) SKOV-3 were transfected with oligofectAMINE alone or with siRNAs corresponding to PI3K-C2 and PI3K-C2? and further transfected with a cDNA encoding GFP-2XFYVEHrs. After 24 h, cells were serum deprived overnight and stimulated with 25 μM LPA for 10 min before fixing for confocal microscopy analysis. Bar, 10 μm. (B) Quantitative analysis of GFP-2XFYVEHrs translocation to the plasma membrane in SKOV-3 as in A. Data are mean ± SEM from three independent experiments. (C) Representative blots of lysates from SKOV-3 transfected with oligofectAMINE or the indicated siRNAs. Levels of PI3K-C2 and PI3K-C2? were assessed by using specific antibodies.
9 _; K. ^6 h8 ^5 L; w; [5 d% j# d2 o8 T! C& D$ Y- y9 ~
Figure 8. PI3K-C2? is a downstream effector of LPA. (A) PI3K-C2? activity was assessed in LPA-stimulated HeLa by in vitro kinase assay as described in Materials and methods. Data are mean ± SEM of three independent experiments. (B and C) HeLa (B) and SKOV-3 (C) were transfected with a cDNA encoding a myc-tagged PI3K-C2?. After 24 h, cells were serum deprived overnight and stimulated with 25 μM LPA for the indicated times before fixing for confocal microscopy analysis. Bar, 10 μm.
% D' `2 W) b& W% ^ ]1 A* y0 a8 r( v1 B- J/ x5 ?! p% P6 R
PI3K-C2? is necessary for LPA-dependent migration
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We investigated the potential role of class II PI3Ks in the LPA-mediated cell migration by performing migration assays on cells transfected with the isoform-specific siRNAs. Wound healing assay monitored by time-lapse microscopy revealed that knockdown of PI3K-C2? expression inhibited cell migration in SKOV-3 (Fig. 9 A). Similar results were obtained in wound healing assay performed in HeLa (Fig. S4 C). In both cases no effect was observed in cells transfected with the PI3K-C2 siRNA. Similarly, knockdown of PI3K-C2? expression completely inhibited cell migration assessed by Transwell assay both in SKOV-3 (Fig. 9 B) and HeLa (Fig. 9 C). Representative fields of migrated HeLa in Transwell assay are shown in Fig. 9 D. The complete inhibition of migration was consistent with the high uptake of siRNAs in these cells (Western blotting analysis for HeLa is shown in Fig. S4 D). These data indicate that PI3K-C2? plays an important role in the LPA-dependent migration.
* D: F$ u- m, g8 Y4 [/ K; l; W" D3 Y6 Q4 _; ^
Figure 9. PI3K-C2? is involved in LPA-mediated cell migration. (A) Time-lapse microscopy of wound healing assay in serum-deprived SKOV-3 transfected with oligofectAMINE or the indicated siRNAs. Images show two opposite sides of the same representative fields of the recorded wounded area at time 0 and at the indicated hours in the presence of 25 μM LPA. (B) Transwell assay of serum-deprived SKOV-3 transfected with oligofectAMINE or with the indicated siRNAs. Data are mean ± SEM of four independent experiments performed in duplicate. (C) Transwell assay of serum-deprived HeLa transfected with oligofectAMINE or with the indicated siRNAs. Data are mean ± SEM of three independent experiments. (D) Representative phase-contrast images of migrated HeLa in Transwell assay as in C obtained by staining the membranes of the Transwell chambers with crystal violet.
! ~9 f' Y" k. Z- q# h
0 a. m. Y3 D0 ~$ W: h/ w+ VDiscussion
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+ R8 ?8 |1 y5 q2 U, P7 d, L/ WLPA activates the class II enzyme PI3K-C2? and generates the lipid second messenger PtdIns-3-P
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A role for PtdIns-3-P as an intracellular second messenger has been suggested by the recent demonstration that this phosphoinositide plays an important role in insulin signaling (Chaussade et al., 2003; Maffucci et al., 2003). Here, we demonstrate that LPA can generate PtdIns-3-P at the plasma membrane of COS7, HeLa, and the ovarian cancer cell line SKOV-3, which indicates that pools of PtdIns-3-P can be generated by different stimuli and therefore PtdIns-3-P might play a more general role in signal transduction. The LPA-mediated generation of PtdIns-3-P seems to be very specific, at least in HeLa and SKOV-3, because in our experimental conditions we did not detect any formation of PtdIns-3,4,5-P3 either by HPLC or TLC analysis. These data indicate that, at least in these cells, formation of PtdIns-3-P does not involve activation of p110 or p110?, the members of class I PI3K most frequently associated with LPA signaling (Yart et al., 2002). Furthermore, generation of this LPA-dependent pool of PtdIns-3-P is sensitive to treatment with 100 nM of the PI3K inhibitor wortmannin but is more resistant to treatment with concentrations of LY294002 up to 10 μM, known to completely block class I PI3K. Such sensitivity to the PI3K inhibitors is consistent with observed in vitro sensitivities of the class II enzyme PI3K-C2? (Arcaro et al., 1998; Fig. S4, A and B). This observation together with our evidence suggesting that the insulin-dependent pool of PtdIns-3-P is generated through a class II PI3K-mediated mechanism led us to hypothesize that the LPA-dependent pool of PtdIns-3-P might be the product of a class II PI3K.
% J" u& Z4 s/ x1 ?) g4 e+ n4 @2 R' W/ @ z A. \* W7 P3 @
There is currently a growing interest in class II PI3Ks because several lines of evidence suggest that different agonists can activate these enzymes; however, their precise intracellular role and their lipid products in vivo still remain to be established (Foster et al., 2003). Here, we show that LPA activates PI3K-C2? and that knockdown of this enzyme specifically inhibits formation of PtdIns-3-P at the plasma membrane upon LPA stimulation. This is the first paper showing that PI3K-C2? is activated and PtdIns-3-P is generated upon LPA stimulation, and therefore, this work identifies a novel PI3K-C2?–PtdIns-3-P pathway in LPA signaling. Furthermore, this study is the first clear indication that PtdIns-3-P might be the in vivo product of PI3K-C2?.% l) F3 T/ P% I- O
/ }' C7 Y' ~: V- [# }5 R! p9 q3 QThe PI3K-C2?–PtdIns-3-P pathway is involved in the LPA-dependent cell migration
2 x1 F3 B9 K1 D3 A- {9 a
2 D9 p0 ~! j6 ]' S7 c4 B$ ]4 }$ U: hThe observation that the sensitivity to PI3K inhibitors of LPA-dependent migration is very similar to the effect observed on PtdIns-3-P formation led us to hypothesize that this pathway might be involved in the LPA-dependent cell migration. As for PtdIns-3-P formation, treatment with 100 nM wortmannin blocked migration whereas 1 μM LY294002 had no effect and 10 μM LY294002 had a partial effect on the LPA-mediated migration. Sensitivity to LY294002 is a key feature of PI3K-C2? that shows a different sensitivity to this inhibitor compared with class I PI3K (Fig. S4 B). In particular, 1 μM LY294002, although able to significantly inhibit class I PI3K, has no effect on the activity of PI3K-C2?. More crucially, a concentration of 10 μM LY294002 completely blocks class I PI3K, whereas it has only a partial inhibitory effect on PI3K-C2?. It is important to underline that 10 μM LY294002 represents a crucial point in the curve of sensitivity of PI3K-C2? that is where the activity of the enzyme significantly drops (Fig. S4 B). The observation that 10 μM LY294002 corresponds to the slope in the curve of sensitivity of PI3K-C2? might explain the variability in the results that in some experiments was associated with the use of such concentration of the inhibitor (e.g., see the high SEM associated with the PtdIns-3-P formation in Fig. 3 A). However, even taking into account such variability, it is clear that both the LPA-dependent generation of PtdIns-3-P and migration are quite resistant to treatment with LY294002, and this finding is consistent with the observed sensitivity of PI3K-C2?.
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& l7 M( B7 E( t& ^Specific blockade of PtdIns-3-P (by overexpression of either a PtdIns-3-P–specific phosphatase or PtdIns-3-P–binding domains) blocks the LPA-dependent migration. In SKOV-3, the observed effects were partial when we counted the total number of migrated cells, and this result is consistent with a partial transfection efficiency of plasmid DNAs in SKOV-3 (50%). However, when we monitored migration only in transfected (green) SKOV-3, we observed a complete inhibition of migration. Similarly, inhibition of migration was complete in HeLa whose transfection efficiency was >80%. Additionally, knockdown of PI3K-C2? in both cell lines inhibits the LPA-mediated migration. Together, these data indicate that this newly identified PI3K-C2?–PtdIns-3-P signaling pathway is necessary for the LPA-dependent cell migration. These data represent the first clear identification of an intracellular role for a member of class II PI3K and they may shed new light on PI3K-mediated intracellular events.
3 [. u4 f2 w' K7 x8 s3 {+ r) w
. A2 B3 K8 K6 C5 NOver the last decade wide interest has been focused on understanding the mechanisms underlying the major steps of cell migration and the signaling pathways regulating them. On the one hand, cell migration plays a crucial role in a variety of biological processes such as morphogenesis, inflammation, and wound healing. On the other hand, migration is involved in several diseases, including cancer metastasis, atherosclerosis, and arthritis. Therefore, the identification of both PI3K-C2? and PtdIns-3-P as important players in cell migration may help clarify both physiological and pathological mechanisms of cell migration and represent a novel target for therapeutic intervention. Several lines of evidence are now revealing the crucial role for LPA as a motility factor. It has been shown that in human pancreatic carcinoma cell lines LPA stimulates cell migration but not proliferation (Stahle et al., 2003). More interestingly, LPA has been identified as a critical component of ascites for the motility of pancreatic cancer cells (Yamada et al., 2004). In addition, it has been reported that the ectoenzyme autotaxin, a cell motility-stimulating ectophosphodiesterase, can generate LPA in the extracellular environment (Umezu-Goto et al., 2002), and both LPA and autotaxin are involved in motility of neoplastic and nonneoplastic cells (Hama et al., 2004). These data, together with the observation that elevated plasma LPA concentrations have been found in patients with ovarian cancer compared with healthy control group (Xu et al., 1995; Westermann et al., 1998) and that prostate cancer cells produce high levels of LPA (Xie et al., 2002), definitively support a role for LPA in the initiation or progression of malignant disease (Mills and Moolenaar, 2003).! H8 P( L9 f, u% T- n0 n
9 Q8 g- ?% T1 C" w8 J, {" AThe discovery of this novel PI3K-C2?–PtdIns-3-P pathway in LPA-dependent migration of different cell lines, including an ovarian cancer cell line, may therefore result useful in elucidating the role of LPA in this disease. Furthermore, because a wide range of mammalian cell types are LPA-responsive, it is tempting to speculate that the PI3K-C2?–PtdIns-3-P pathway might have a more general role in different biological contexts.' x' U# F* ]! t
5 G, q# z& s: [; ~' V2 W" c) bMaterials and methods
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( D8 P4 K. U- Z+ N( o5 pMaterials and plasmids1 m4 n, o' t1 G# W
/ e7 A7 s3 H0 WL--LPA, oleoyl (C18:1,-9), anti-myc, anti–?-actin, and anti-Flag were purchased from Sigma-Aldrich; anti-Akt, anti-pSer473Akt, and anti-pThr308 from Santa Cruz Biotechnology, Inc.; anti-HA from Boehringer; anti-p85 from Upstate Biotechnology; anti-PI3K-C2? and anti-GFP from BD Biosciences; and Alexa 594 phalloidin from Molecular Probes. Anti-PI3K-C2 was a gift of P.R. Shepherd (University of Auckland, Auckland, New Zealand). Flag-MTM1 and Flag-MTM1 C375S were a gift of J. Laporte (Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France). myc-PI3K-C2? was provided by M.D. Waterfield (The Ludwig Institute for Cancer Research, London, UK). Chemically synthesized double-stranded siRNAs with 19-nucleotide duplex RNA and 2-nucleotide 3' dTdT overhangs were purchased from Dharmacon or Eurogentec. To design class II PI3K-specific siRNAs, the human PI3K-C2 (GenBank/EMBL/DDBJ accession no. NM 002645) and PI3K-C2? (GenBank/EMBL/DDBJ accession no. NM 002646) mRNA sequences were searched for 21-nucleotide unique sequences using the National Center for Biotechnology Information database and the BLAST search algorithm. The cDNA sequences against which siRNAs were prepared are 5'-AAGTCCAGTCACAGCGCAAAG-3' (nucleotides 1224–1244) for PI3K-C2 and 5'-AAGAATGCGACGCCTGGCAAG-3' (nucleotides 1368–1388) for PI3K-C2?.
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: w! t$ ?; k$ Z8 S5 jCell cultures and transfection/ ^1 R! t1 S, g
( l2 h4 p: O' w2 f+ f/ sCells were maintained in DME supplemented with 10% FBS and transfected with LipofectAMINE PLUS (Invitrogen) according to the manufacturer. Transfection with siRNAs was performed as described previously (Elbashir et al., 2002) and cells were used after 48–72 h.
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HPLC analysis" G8 R7 [: e8 C& h( ?% C5 X
& |1 }2 M- }6 Z1 J$ K3 H0 w1 RCOS7, HeLa, and SKOV-3 were incubated with 10 μCi/well myo-inositol (PerkinElmer) in inositol-free medium M199 for 24 h, and then left untreated or stimulated with LPA for different times. HPLC analysis was performed as described previously (Maffucci et al., 2003).7 e2 z' `6 u4 |" |9 B' Q
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TLC analysis
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$ \& |% X7 Z6 T1 c3 ZSerum-starved cells were incubated with 0.8 mCi/well phosphoric acid (PerkinElmer) in phosphate-free medium, M3786, for 3 h before stimulation as indicated. Phospholipids were extracted, dried under nitrogen, resuspended in 100 μl chloroform/methanol (3:2), and spotted onto silica plates presoaked in water/methanol (3:2) and 1.2% (wt/vol) oxalic acid, and oven baked for 15 min at 110°C. TLC plates were developed in chloroform/acetone/methanol/acetic acid/water (90:36:30:27:18).
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+ ]8 q3 u& {! p% p% h, YConfocal microscopy and quantitative analysis
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8 i$ I t% Z2 h9 ~; y( {! yCells grown on glass coverslips were fixed with PBS containing 4% PFA for 10 min at RT. Where necessary, cells were permeabilized with 0.25% Triton X-100 and incubated with an anti-myc antibody followed by an FITC secondary antibody. Microscopy was performed by using a laser confocal microscope system (TCS NT; Leica) connected to a microscope (model DMXRE; Leica) equipped with a 63x water immersion objective. Images were acquired and loaded by using a TCS NT program (version 1.6.587). No further processing of the images was done except for changes in brightness/contrast to better visualize the data. For quantitative analyses of GFP-2XFYVEHrs translocation, GFP-2XFYVEHrs-transfected cells were serum starved for 24 h and stimulated with LPA for the indicated times before fixing for confocal microscopy. At least 100 cells/coverslip were analyzed by blind scoring (no score: cells with 0–25% membrane localization; score 1: cells with >25% membrane localization). The number of cells showing a plasma membrane localization of the GFP-2XFYVEHrs was expressed as a percentage of control. Statistical analyses were performed by paired t test.: ^. a0 c' R6 {* z. E( q' L
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PI3K assay
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# \5 N8 M# i) q1 B% {# DPI3K activity assay was performed as described previously (Kamalati et al., 2000).
: O r% h1 ?$ f2 D3 n
! A0 T9 q1 n- K) KMigration assays
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+ ?1 a/ y% a" c; P: x) z3 p0 VWound healing assay.7 W5 |' A! G0 E) u/ b% y3 [; X
) n) v# B+ `9 [% U* A0 g
Confluent cells were serum deprived overnight, left untreated or treated as indicated, and then wounded with a linear scratch by a sterile pipette tip. After washing, cells were incubated in the presence or absence of LPA and 0.5 μg/ml mitomycin-C. Time-lapse microscopy was performed by using a microscope (model Eclipse TE2000-U; Nikon) equipped with a temperature controller (Solvent Scientific; 37°C). No further processing of the images was done except for changes in brightness/contrast to better visualize the data. Cell movement was recorded with a charge-coupled device camera (OrcaER; Hamamatsu Photonics) by using PCI Software (Digitalpixel). To obtain the quantitative analyses of Fig. 4 B and Fig. 5 B, panels as those shown in Fig. 4 A and Fig. 5 A were prepared for each experiment. Each panel was made up of images of cells collected at time 0 and every hour (1–17 h) for each condition (control, LPA, and different concentrations of PI3K inhibitors LPA). The wounded area was defined in each image by positioning red lines in correspondence of the original scratch. The number of cells migrated into the wounded area at different hours was visually counted. Alternatively, the wounded cell monolayers were photographed using a microscope (Nikon). For quantitative analysis shown in Fig. 6 I, experiments were performed in cell culture dishes with a 2-mm grid (Nunc) and migration was quantified by counting the number of cells per grid square (4 mm2) by using a microscope (Nikon).# N# x9 [* |6 a. A1 c
) t7 Q& M$ w9 {% w, v8 rTranswell assay.
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Alternatively, cell migration was performed in Transwell chambers (tissue culture treated, 10-mm-diam, 8 μm pores; Nunc) coated with 100 μg/ml of type I collagen. Cells were resuspended in DME containing 1% BSA, added (30,000 cells/150 μl) to the top of each migration chamber, and allowed to migrate in the presence of LPA in the lower chamber. After 4 h, cells that had not migrated were removed using a cotton swab, whereas migrated cells were fixed with 4% PFA. To count the total number of migrated cells, the membranes of the Transwell chambers were stained with 1% crystal violet and analyzed by phase-contrast microscopy using a microscope (Nikon; examples are shown for HeLa in Fig. 9 D). To count only the transfected cells, we took advantage of the observation that they expressed GFP-tagged proteins. Therefore, the Transwell chambers were analyzed by fluorescent microscopy using a microscope (model DMRXE; Leica) equipped with a 10x objective. In this way, only fluorescent (green) cells that had migrated were visualized and counted.
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" h1 C9 K/ b9 P, c7 C- lCell growth
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- ^* n7 O! e6 v- J6 q, W4 y104 cells/well in a 48-well plate were serum deprived overnight before adding LPA for a further 48 h. thymidine (0.5 μCi/well) was added for the last 18 h. After washing with ice-cold PBS, wells were incubated with 5% TCA at 4°C for 20 min, washed with PBS and methanol, and air dried. After 30 min in 0.2 M NaOH, samples were collected and radioactivity was assessed by scintillation counting. Alternatively, MTT assay was performed as described previously (Piccolo et al., 2004).5 ^" t6 j, X4 m
) ?+ T' b: {# g2 _! m3 P- C3 NPhospholipase C activation$ U4 J1 h. _+ k% T" T2 s
% C2 y5 K( x% Q! M2 }& l
Cells were incubated with 2 μCi/well myo-inositol (PerkinElmer) in M199. After 24 h, cells were incubated in M199 plus 10 mM Hepes and 20 mM LiCl for 15 min before stimulation, scraped in 0.1 M HCOOH, and the total amount of inositol phosphates was assessed using AG 1-X8 resin (formate form, 200–400 mesh; Bio-Rad Laboratories) and liquid radioactivity counting.5 c U% b, P4 Y/ s$ n
. |8 e1 A& ?" Z* AOnline supplemental material
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Fig. S1, Fig. S2, Fig. S3, Fig. S4 and the corresponding legends are available in the online supplemental material. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200408005/DC1.
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Acknowledgments
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We thank Professor M.A. Horton, Dr. G. Schiavo, and Professor P.R. Shepherd for critical reading of the manuscript; The Ludwig Institute for Cancer Research for the myc-tagged PI3K-C2? construct, Dr. J. Laporte for MTM1 constructs, Professor P.R. Shepherd for anti–PI3K-C2 antibody, and Mike Lau for help with the experiments in Fig. S4 (A and B).% [% d. @ f9 b( e
2 S" r" v6 M1 O* \" G% S* @" S
This work was supported by the Association for International Cancer Research (grant 05-127 to M. Falasca), Compagnia di San Paolo (to M. Falasca), and Diabetes UK (grant BDA:RD02/0002388 to M. Falasca). T. Maffucci is the recipient of a Diabetes UK RD Lawrence Fellowship (grant BDA:RD04/0002884). F.T. Cooke, F.M. Foster, and C.J. Traer are supported by the Wellcome Trust, the Biotechnology and Biological Sciences Research Council (grant 4S/C14421 to M.J. Fry), and a Research Endowment Trust Funds studentship from the University of Reading, respectively. M. Falasca is supported by an endowment from the Dr. Mortimer and Mrs. Theresa Sackler Trust.. H5 f$ C; ^/ }# x+ @' t
: F. k( i2 ?; B/ M% X! hThe authors declare that there are no conflicts of interest regarding this article.& q ^9 V* u5 x& W
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