诱导+球培养稳定的癌干细胞株

csc1981

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Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):6097-102. doi: 10.1073/pnas.1118262110. Epub 2013 Mar 11.
+ A8 p1 K+ \& x: }0 k. JTransient treatment with epigenetic modifiers yields stable neuroblastoma stem cells resembling aggressive large-cell neuroblastomas.
, h3 l% F& u) r' i* hIkegaki N1, Shimada H, Fox AM, Regan PL, Jacobs JR, Hicks SL, Rappaport EF, Tang XX.. ?1 W- p- p8 [4 ?* f9 {
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0 ~" D2 r4 z! g- lAbstract
- f% y- ~$ Z& l/ ?/ o& dCancer stem cells (CSCs) are plastic in nature, a characteristic that hampers cancer therapeutics. Neuroblastoma (NB) is a pediatric tumor of neural crest origin, and half of the cases are highly aggressive. By treating NB cell lines [SKNAS, SKNBE(2)C, CHP134, and SY5Y] with epigenetic modifiers for a short time, followed by sphere-forming culture conditions, we have established stem cell-like NB cells that are phenotypically stable for more than a year. These cells are characterized by their high expression of stemness factors, stem cell markers, and open chromatin structure. We referred to these cells as induced CSCs (iCSCs). SKNAS iCSC and SKNBE(2)C iCSC clones (as few as 100 cells) injected s.c. into SCID/Beige mice formed tumors, and in one case, SKNBE(2)C iCSCs metastasized to the adrenal gland, suggesting their increased metastatic potential. SKNAS iCSC xenografts showed the histologic appearance of totally undifferentiated large-cell NBs (LCNs), the most aggressive and deadly form of NB in humans. Immunohistochemical analyses showed that SKNAS iCSC xenografts expressed high levels of the stem cell marker CXCR4, whereas the SKNAS monolayer cell xenografts did not. The patterns of CXCR4 and MYC expression in SKNAS iCSC xenografts resembled those in the LCNs. The xenografts established from the NB iCSCs shared two common features: the LCN phenotype and high-level MYC/MYCN expression. These observations suggest both that NB cells with large and vesicular nuclei, representing their open chromatin structure, are indicative of stem cell-like tumor cells and that epigenetic changes may have contributed to the development of these most malignant NB cells.

csc1981

这种方法有什么优缺点吗？能否用于其他稳定的癌干细胞株的诱导培养呢？
0 A3 d+ T2 _: ?6 Q& H6 w/ d- p特点一：稳定性如何判定
. c+ |& {$ f7 P& a: k" S特点二：干细胞株如何判定2 X6 d7 J9 D" {: n( K4 n
欢迎讨论，谢谢