FGF8-FGFR1是保持小鼠精原干细胞未分化状态的生境因子

cartoonyang

FGF8-FGFR1 Signaling Acts as a Niche Factor for Maintaining Undifferentiated Spermatogonia in the Mouse1$ M) e) }$ I  u! r9 U
0 U8 r) v8 ?* Z& p  j3 I8 c! \
Published online before print October 30, 2014, doi: 10.1095/​biolreprod.114.121012
6 G0 @2 \* r8 r3 N! r  v" VBiology of Reproduction December 1, 2014 vol. 91 no. 6 145
3 a  X7 I# C' N0 {3 b% q3 b+ F3 ]) [
ABSTRACT$ n; a: m. `+ E* x' R
; J5 X8 {1 ]1 D8 r- M" F$ w( \$ S; e
In mammalian testes, spermatogonial stem cells (SSCs) maintain spermatogenesis over a long period of time by undergoing self-renewal and differentiation. SSCs are among the most primitive of spermatogenic cells (undifferentiated spermatogonia), and their activities are strictly regulated by extrinsic niche factors. However, the factors that constitute a testicular niche remain poorly understood. In this study, we demonstrate that fibroblast growth factor (FGF) signaling maintains undifferentiated spermatogonia through activating ERK1/2 signaling in vivo. Undifferentiated spermatogonia comprise GFRA1+ and NANOS3+ subpopulations, which are likely to undergo self-renewal and enter the differentiation pathway, respectively. In the testis, Fgfr1 was expressed in the entire population of undifferentiated spermatogonia, and deleting FGFR1 in spermatogenic cells partially inactivated ERK1/2 and resulted in reduced numbers of both GFRA1+ and NANOS3+ cells. In addition, Fgf8 was expressed in spermatogenic cells, and loss- and gain-of-function models of FGF8 demonstrated that FGF8 positively regulated the numbers of undifferentiated spermatogonia through FGFR1, particularly among NANOS3+ cells. Finally we show a possible involvement of FGF signaling in the reversion from NANOS3+ into GFRA1+ undifferentiated spermatogonia. Taken together, our data suggest that FGF signaling is an important component of the testicular niche and has a unique function for maintaining undifferentiated spermatogonia.$ e2 F" y# t; K" R  y% \+ r. t
3 V+ X" [" D* [7 o- {
Key words: developmental biology knockout model Sertoli cells signal transduction spermatogonial stem cells transgenic model
# L, [) }2 F5 K+ Q8 c
7 K- ~) i0 w0 D

yeshch

thanks a lot

yeshch

thanks a lot

yeshch

求原文，亲爱的楼主