ips的克隆不长大是什么原因

mecol

我用病毒转染的方案，看到了克隆，但是一直没见克隆长大，是什么原因啊？

watchen198891

能上图给看看吗？你有把克隆挑出来单独培养？

ciweiyuan

营养不够，检查滋养层细胞状态

yuanyecat

也可以用不用滋养细胞的培养基

lchanlon

回复 mecol 的帖子
- ^7 L. k2 |  e2 O. f
7 E/ C" W) B+ S; `I would say the clone you got is not a fully reprogrammed iPSC. When somatic cells received reprogramming factors, cells were going to be different stages. They might die, apoptosis, transform, senescence et al. It is a very heterogeneous population. Some cells started to reprogram, but they stopped at different stages. Some cells go to a stage to iPSC, then go back to somatic status. Only a very small percentage of cells ultimately become fully iPSC.

无涯

有没有图片啊，你已经诱导多少天了，是不是MEF的使用时间太长了