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- 积分
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- 858
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O/ O) H1 E9 @1 I; l0 `7 c
(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g& [% _8 F' B7 B7 Q$ ~
MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),
' H1 n; S# g: G, b4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;
" Q. k# ^3 ?% t2 F9 sSigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no./ F9 o* m, F7 Y& p
G6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g0 y7 H. G; w' }# R# _' R
penicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate
& z" M; q$ k/ L) r(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of& x# Y) J, n5 ?+ o
Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L
( U* c$ g* b, W; U( m( P' `with Milli-Q water. |
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
