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- 积分
- 264
- 威望
- 264
- 包包
- 858
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O
0 [" J5 w9 ^7 d( z(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g
7 ?) R( J* z( U; ?2 X. }MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),, a9 C/ U! N& P7 g" W
4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;. J' R- N0 K7 b8 g" V% B
Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.
( G: b* A8 p, KG6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g7 l# A* k# @7 K: i9 q8 G i
penicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate V# V. Y2 x7 U' X2 e5 _
(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of& }# ~$ N! L' Z8 ]9 S% s2 O" q0 \
Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L9 f( k5 |9 G$ X3 _0 O$ b+ z- _
with Milli-Q water. |
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
