PDF电子书：Current Protocols in Stem Cell Biology 2007

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$ _- `  o+ m. x$ V4 X$ i( z; c/ rFOREWORD7 `3 ~# t: q- V' P
Stem cell biology is emerging as a field in biology with tremendous therapeutic potential.
0 b+ p' u  m; wMaking this potential a reality requires an international effort. The recognition
. {6 y+ {; o1 [5 `5 Dthat such a promising yet multifaceted discipline needs fostering led to the establishment1 @/ r4 C, O: _+ m- r8 P" b) e" c
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the
) U' N5 Y- O% {3 h$ a5 yISSCR is the development of tools to ensure the success of stem cell researchers. What7 H; D- P9 x1 p: v, W
better way to do this than to collaborate with Current Protocols to develop this valuable
# h5 e! {+ k) b7 E$ Qcompendium of protocols in stem cell biology?8 M- p% j9 R' m# ]7 C* R' C/ I1 B- r) E2 F
Stem cell researchers have developed a number of breakthrough techniques, from the0 u2 O4 f( l$ I- e
derivation and manipulation of pluripotent embryonic stem cells to purification and
3 u( @. C, ~# ]$ f1 V# Stransplantation of tissue-restricted stem cells from adult organs. A number of laboratories( s( K% J# }" T  D$ b4 z5 V9 Q- J
have become leaders in the field as a result of developing such techniques. The more
  K8 G4 ^" y7 G- e: Xefficient scientists are at implementing new and powerful methodologies in their own
( x/ e1 h& h1 x; A9 slaboratories, the faster stem cell biology will advance our understanding of normal
+ R/ S7 v- C/ R, t0 ~& y; O2 Udevelopment and lead to the development of therapies. Thus, the availability of quality. C) {% i2 {& I
protocols will have amajor impact on the success of the entire field. Current Protocols has, o% ?4 i$ E/ O( N! w
long been the premier volume for proven in-depth protocols regarding many aspects of
( N' |/ N6 V3 G% sbiology, and this volume on stem cell biology will prove a valuable addition to researchers6 k" y- j0 ]: c8 U' r
worldwide.
8 o+ W4 z6 g: \Experiments in stem cell biology must be interpreted with great caution as well as
* U. W3 _& V; r- ]* oopenness to alternative explanations. For example, the recently discovered phenomenon! s. y# N0 Y* X
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
, n7 q% N( J! h9 F- h; r9 }+ s! `" Atissues were initially misinterpreted as evidence for stem cell trans-differentiation.
4 u9 o! \* A5 z1 ?9 _, qIt is very important that this compendium of protocols highlight potential pitfalls as well
* O1 w( h$ g7 [as maintain the opportunity for clarification and correction when the need arises. The; t1 f, J3 w7 C) r6 T" N! z
fact that these protocols will be provided online will help ensure that researchers always
/ i; ^8 Z2 }; f! d7 L) ahave the latest, most up-to-date protocols available to them., g6 h% ~" u3 l, g2 Z9 J" @
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine
, y" {3 t! c% i% bpush to share information and interact so that the field can move forward quickly. There
7 \; Y2 Z, t5 u# I; M0 ?* a; b& E3 @is a drive to develop not only excellent basic research skills but to bring the findings$ \, v, k4 Q7 y8 j- I5 _
to clinical use so that patients can benefit. As a Hematology Attending Physician at
7 s' K" H3 q$ [( v" YChildren’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia
# {$ @) k2 s" W% i9 ?and am drawn by the need to translate our research findings into therapies to help treat a
; C; y# V' X* e7 y* w2 mnumber of diseases. The promise is great, but we need to deliver, and I believe Current
$ t% o  M2 P2 i  d  ^: TProtocols in Stem Cell Biology will help tremendously.
4 M& G. m1 \% T' N4 o( CLeonard I. Zon" _5 g  q$ h- Y5 L- q) {" S
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