常用的β-actin 引物序列

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human actin f ctc cat cct ggc ctc gct gt % a; v0 R% ?% p+ O8 n
human actin r gct gtc acc ttc acc gtt cc , G' C) Y* F+ \) g
product size:268
) ~  U# |9 K. R8 J. Krabbit actin r agt gcg acg tgg aca tcc g
4 Z% [+ F/ {# L9 e6 [rabbit actin f tgg ctc taa cag tcc gcc tag ( z+ v. Z8 B; r
product size:295 1 F7 J" }- k& }3 d( N
mouse actin r cgt tga cat ccg taa aga cc * x# G" p  M9 H4 r! w
mouse actin f aac agt ccg cct aga agc ac
$ ?3 K1 Z6 R  J' R+ X9 l  oproduct size:281
* m6 h# D. }8 [* Q6 r8 hrat actin f TCAGGTCATCACTATCGGCAAT
0 O6 C5 D; M# W. n0 C2 F  _5 Srat actin r AAAGAAAGGGTGTAAAACGCA
) L$ X) z* D; h8 ]9 J2 fproduct size:432
/ [! g2 @1 P! B0 H' phuman actin r gag cta cga gct gcc tga cg
- j. ?' }' ]6 m' ohuman actin f cct aga agc att tgc ggt gg
8 X/ q# M1 A2 I6 ~0 kproduct size:416
1 S' a9 v# ~! s+ _% T% Kmouse actin f tca tca cta ttg gca acg agc - E2 U( s' K4 P  A! H+ m
mouse actin r aac agt ccg cct aga agc ac + [6 Q$ \+ u8 |( e2 K; B
product size:399
( B' o/ y5 W2 o# ?* {& Prat actin f CCCATCTATGAGGGTTACGC
' q4 u* R, j( _4 Y! v( M$ [  rrat actin r TTTAATGTCACGCACGATTTC 2 e1 ]! e; F# N" C
product size:150
/ Z+ v$ x) [) z% nrabbit actin f tct tcc agc cct cct tcc tg
# w: c1 N! Q' H& ?rabbit actin r cgt ttc tgc gcc gtt agg t # [6 T% }* s6 s+ J) h8 Z
product size:409 , P9 w3 T4 a0 q
内参基因名称 引物 引物最佳退火 扩增 - t5 g; V. _1 X  D
基因库序列号 引物名称 序列 位置 Tm 温度C 长度
& @4 {, ]0 c5 m0 f7 q. c+ w
  B5 ?/ ]# X3 T! rHuman actin beta F305 ctgggacgacatggagaaaa 305-324 52.3 , G. r3 L- \" L% U0 W1 V1 ]
BC002409 R868 aaggaaggctggaagagtgc 868-849 52.6 59.4 564
6 w8 Q6 z( W$ r: u1 }: \F1379 agcgagcatcccccaaagtt 1379-1398 57.3
% ]3 ~% [$ N+ p) FR1663 gggcacgaaggctcatcatt 1663-1644 56.3 54 285 4 K% Z; N( T" H& l+ S

8 m% d" z% _& R% V' H+ J: P! hRat actin beta F18 cacccgcgagtacaaccttc 18-37 54.5
" I0 N% X! U: _7 d9 }NM_031144 R224 cccatacccaccatcacacc 224-205 54.4 60.4 207 , Y: V( ~' N9 X) q0 U. H1 \
F694 gagagggaaatcgtgcgtgac 694-714 54 ( f* K1 l5 R' N
R1146 catctgctggaaggtggaca 1146-1127 53.2 57.1 452 ; c3 K7 D% }! w) r- @, K6 v
# T; n; `+ n8 i# e5 u) k0 X
Mouse actin beta F91 atatcgctgcgctggtcgtc 91-110 57.5 ; w1 j# G0 [9 z5 e% B" {& p
NM_007393 R607 aggatggcgtgagggagagc 607-588 57.8 60.4 517 2 j( |2 \" B2 e" q; U4 k
F1566 gtccctcaccctcccaaaag 1566-1585 54.5 1 P3 p$ {1 e* _3 c% E" K# E
F1831 gctgcctcaacacctcaaccc 1831-1811 54.4 55.7 266
; i) s9 Y" a) a% E. _2 A
' \' y! D  |+ m- Q) f% Uhuman GAPDH F369 agaaggctggggctcatttg 369-388 55.6
) q/ M5 W: R1 K) d% `* bBC004109 R626 aggggccatccacagtcttc 626-607 55.1 57.5 258 6 Y$ F! j2 R6 N+ p: j% `. J. e& z' y
F66 aggtcggagtcaacggatttg 66-86 52.5
9 c4 G( G1 I" L" Z$ QR597 gtgatggcatggactgtggt 597-578 52.6 57 532
7 V; `; V/ Y/ v6 e8 x
! i4 s3 R" Q( U& s# L) ?8 l0 i$ ZRat GAPDH F50 cagtgccagcctcgtctcat 50-69 55
- v& ~3 f5 d9 F% v$ v' ]BC059110 R644 aggggccatccacagtcttc 644-625 55.1 58.3 595 - v$ o( v; f1 y, \  s
F1029 acagcaacagggtggtggac 1029-1048 54.4 - F7 t- `) B0 t0 t
R1280 tttgagggtgcagcgaactt 1280-1261 54.8 57.7 252
4 \  @4 o$ W" i; n: V8 M! I4 g5 F8 x$ U& P: N  ~; R6 p  L* O  X
Mouse GAPDH F306 aggccggtgctgagtatgtc 306-325 54 1 D# P# W3 q: Y4 Y# |3 p$ r
M32599 R835 tgcctgcttcaccaccttct 835-816 54.6 58.7 530
7 |9 ?/ j* w) V8 v- H! C# j6 jF49 ggtgaaggtcggtgtgaacg 49-68 55.1
, ]+ @* H) \$ g8 u3 q2 T4 dR281 ctcgctcctggaagatggtg 281-262 54.5 56.8 233
  z% t$ h# y$ p5 ^5 U: X2 j2 e
2 d. ?: D' u4 X5 \, h8 I4 [Rat 18S rRNA F1865 tgcggaaggatcattaacgga 1865-1886 58.6 - X( R, z2 c! Y$ |) G
M11188 R2164 agtaggagaggagcgagcgacc 2164-2143 59 59.3 300 6 l$ a5 C9 X( u& R
) B! T& V5 B* t$ d
human 18S rRNA F1565 cagccacccgagattgagca 1565-1584 58.3 & ^2 {) h2 e" i+ l
M10098 R1816 tagtagcgacgggcggtgtg 1816-1797 58.3 59 253 9 `0 m3 y+ ], v) b& q- B% q2 K, }

( C' c$ X7 ^& e9 w: k" u% fmouse 18S rRNA F56 aggggagagcgggtaagaga 56-75 54.4 9 X# ^1 J9 G& R% X+ F
K013364 R296 ggacaggactaggcggaaca 296-277 54 62 241