求助2篇nature protocol文献，已给链接，辛苦！！！多谢了！！！

sunyucheng

（1）http://www.nature.com/nprot/journal/v5/n3/pdf/nprot.2009.236.pdf
$ K6 L9 b  q1 a( `( b% M* u. GNature Protocols 5, 516–535 (1 March 2010) | doi:10.1038/nprot.2009.236
& C) c9 w8 ]  B" BRNA-Seq analysis to capture the transcriptome landscape of a single cell.
& a( w! r3 |+ l1 E（2）http://www.nature.com/nprot/journal/v4/n11/pdf/nprot.2009.119.pdf% Y" G$ Z) h4 S3 |
A reporter gene imaging system for monitoring microRNA biogenesis .
7 I3 J' k2 x0 j  rNature Protocols 4, 1663–1669 (1 October 2009) | doi:10.1038/nprot.2009.119 & X% {. G+ m7 M/ `
（3）ttp://www.nature.com/nprot/journal/v4/n9test/pdf/nprot.2009.145.pdf! N* V5 H( G4 `' j: T" c6 s7 O
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression .
( m! G9 I9 s5 v( x6 x" uNature Protocols 4, 1338–1348 (1 August 2009) | doi:10.1038/nprot.2009.145
' C/ P9 }+ L: M, p5 F（4）http://www.nature.com/nprot/journal/v4/n7/pdf/nprot.2009.95.pdf
4 T# x* a6 K$ H! W$ W' q* k: S1 dConditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase .) P2 ^/ F/ j' _* l$ T' n
Nature Protocols 4, 1064–1072 (1 June 2009) | doi:10.1038/nprot.2009.95 7 v5 Y  R+ X9 d7 v: S" A% t
（5）http://www.nature.com/nprot/journal/v3/n9/pdf/nprot.2008.140.pdf# }' S* Q+ l+ L+ B+ T
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification .3 }/ w# P/ @0 l* L1 m4 E
Nature Protocols 3, 1435–1443 (1 August 2008) | doi:10.1038/nprot.2008.140
$ g1 A7 P! l2 [* [5 T（6）http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.67.pdf' T5 z  s: `2 I/ P5 ?. [: `) ?+ M
Improved northern blot method for enhanced detection of small RNA 9 g1 F% n& X' a& \8 k! l* U
Nature Protocols 3, 1077–1084 (1 June 2008) | doi:10.1038/nprot.2008.67
  x3 p+ ^% J6 L1 _（7）http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.73.pdf! z( }  A# B4 o* k
Analyzing real-time PCR data by the comparative CT method .0 |  }* A5 A$ ]5 v' s; n! k1 `( x+ D
Nature Protocols 3, 1101–1108 (1 June 2008) | doi:10.1038/nprot.2008.73 / k/ O- O7 M$ N6 i! u
（8）http://www.nature.com/nprot/journal/v3/n4/pdf/nprot.2008.14.pdf- d: i! V4 T# J' |0 k" d& L' I+ W
MicroRNA expression profiling using microarrays .: p" J1 A) [' T1 l+ P0 k0 K2 G
Nature Protocols 3, 563–578 (1 April 2008) | doi:10.1038/nprot.2008.14

hansey

回复 1# sunyucheng

hansey

回复 2# hansey

hansey

回复 3# hansey

hansey

回复 4# hansey

hujimmy623

58062782

谢谢

sunyucheng

太强了，多谢帮助！！！

nculsa747

非常感谢二楼的战友！

yihandaniel

i need these too