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（1）http://www.nature.com/nprot/journal/v5/n3/pdf/nprot.2009.236.pdf) H4 p G" u) A& H9 K% E
Nature Protocols 5, 516–535 (1 March 2010) | doi:10.1038/nprot.2009.236
. i6 X/ n" C; k/ \5 SRNA-Seq analysis to capture the transcriptome landscape of a single cell.
) ^+ f) b+ j% Z- M% G# s（2）http://www.nature.com/nprot/journal/v4/n11/pdf/nprot.2009.119.pdf
8 [" a" u% a! j7 \& R! aA reporter gene imaging system for monitoring microRNA biogenesis ./ x) f& y# d" G* M% \ V5 t; u
Nature Protocols 4, 1663–1669 (1 October 2009) | doi:10.1038/nprot.2009.119
6 @1 h8 T& H% E; O, k7 e（3）ttp://www.nature.com/nprot/journal/v4/n9test/pdf/nprot.2009.145.pdf
. K# j/ _& D% Q6 z% a/ {' O: OSimultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression .
1 ?5 w' `7 m/ c6 K" z0 KNature Protocols 4, 1338–1348 (1 August 2009) | doi:10.1038/nprot.2009.145 i1 H$ `5 k! V/ |
（4）http://www.nature.com/nprot/journal/v4/n7/pdf/nprot.2009.95.pdf& D! p/ t X7 V5 `$ d2 t" ~
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase .
7 Z8 _: r a7 ONature Protocols 4, 1064–1072 (1 June 2009) | doi:10.1038/nprot.2009.95
, Y: _% H$ P+ ?8 I7 [: u（5）http://www.nature.com/nprot/journal/v3/n9/pdf/nprot.2008.140.pdf
' E7 ]: S4 J/ s- q) Y7 N# LFeeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification .- E0 j0 a5 v$ M* K- C. n
Nature Protocols 3, 1435–1443 (1 August 2008) | doi:10.1038/nprot.2008.140
u* [/ C/ d; Q' k（6）http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.67.pdf& x b) a* P9 [
Improved northern blot method for enhanced detection of small RNA
4 r- T( F U5 X6 X( V: S/ C0 `, Z( ]Nature Protocols 3, 1077–1084 (1 June 2008) | doi:10.1038/nprot.2008.67 % @5 D' F, A: R4 C/ i
（7）http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.73.pdf
- r1 y) D$ M8 x4 x, yAnalyzing real-time PCR data by the comparative CT method .
* g% i( N& P- d8 L4 _Nature Protocols 3, 1101–1108 (1 June 2008) | doi:10.1038/nprot.2008.73
1 d# t/ t/ u" y1 p x1 ^2 M（8）http://www.nature.com/nprot/journal/v3/n4/pdf/nprot.2008.14.pdf# Q4 D. }, l: p
MicroRNA expression profiling using microarrays .! k) i! E5 B0 m0 Q# P1 }
Nature Protocols 3, 563–578 (1 April 2008) | doi:10.1038/nprot.2008.14 |
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发表于 2010-3-1 22:32
