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(1)http://www.nature.com/nprot/journal/v5/n3/pdf/nprot.2009.236.pdf, v! X1 D( q& z% g
Nature Protocols 5, 516–535 (1 March 2010) | doi:10.1038/nprot.2009.236 % c4 N% r( _2 l
RNA-Seq analysis to capture the transcriptome landscape of a single cell.2 [4 e5 @( a! G1 d( c3 K' O: i
(2)http://www.nature.com/nprot/journal/v4/n11/pdf/nprot.2009.119.pdf
2 U& N0 j5 Q* n7 o# BA reporter gene imaging system for monitoring microRNA biogenesis .' ]5 }- b% }( @' A" o/ k
Nature Protocols 4, 1663–1669 (1 October 2009) | doi:10.1038/nprot.2009.119 1 o1 z7 E) O; |2 U
(3)ttp://www.nature.com/nprot/journal/v4/n9test/pdf/nprot.2009.145.pdf5 g) _) t2 Q+ J; e I
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression ./ Q) ~( `$ S0 ?" I' |
Nature Protocols 4, 1338–1348 (1 August 2009) | doi:10.1038/nprot.2009.145 # w7 Q4 R* G* e
(4)http://www.nature.com/nprot/journal/v4/n7/pdf/nprot.2009.95.pdf
) ^1 N( |% w" h1 M3 [6 h FConditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase ./ ? j9 _; Z/ T
Nature Protocols 4, 1064–1072 (1 June 2009) | doi:10.1038/nprot.2009.95 / u0 n5 Q r# E$ g% |- U+ Z! J
(5)http://www.nature.com/nprot/journal/v3/n9/pdf/nprot.2008.140.pdf
; a7 p9 g, X; J( uFeeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification .$ K) q$ q& m9 v- {# n
Nature Protocols 3, 1435–1443 (1 August 2008) | doi:10.1038/nprot.2008.140 0 u" ~9 s0 e* d1 Y
(6)http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.67.pdf8 d. ^" X, D9 M' h' Q
Improved northern blot method for enhanced detection of small RNA 8 {# q& Y. \8 c4 u* `) N% Y' ~# U
Nature Protocols 3, 1077–1084 (1 June 2008) | doi:10.1038/nprot.2008.67 ) l0 b7 c! {2 L" h! v4 l- s+ k
(7)http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.73.pdf+ h: d: _1 Z/ D" R; _
Analyzing real-time PCR data by the comparative CT method .
. V. h3 c( j2 {' w* u4 cNature Protocols 3, 1101–1108 (1 June 2008) | doi:10.1038/nprot.2008.73 + M" X* r- x) l8 H- e
(8)http://www.nature.com/nprot/journal/v3/n4/pdf/nprot.2008.14.pdf' B$ m% c* d! U; w& S( e, c( c
MicroRNA expression profiling using microarrays .% \, J8 `2 o# e- V A2 d
Nature Protocols 3, 563–578 (1 April 2008) | doi:10.1038/nprot.2008.14 |
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