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（1）http://www.nature.com/nprot/journal/v5/n3/pdf/nprot.2009.236.pdf
+ b/ I! Z& ^0 c5 R N( Q% S5 ZNature Protocols 5, 516–535 (1 March 2010) | doi:10.1038/nprot.2009.236
, ~: e, X# K7 N' [/ o. |( e( bRNA-Seq analysis to capture the transcriptome landscape of a single cell.; e8 m4 O* z/ ]4 o
（2）http://www.nature.com/nprot/journal/v4/n11/pdf/nprot.2009.119.pdf
5 Z2 w& e# _/ Z- Q' ZA reporter gene imaging system for monitoring microRNA biogenesis .7 Q# I: f% z# M: Q* A
Nature Protocols 4, 1663–1669 (1 October 2009) | doi:10.1038/nprot.2009.119
" z) n, G* A o6 Q1 T（3）ttp://www.nature.com/nprot/journal/v4/n9test/pdf/nprot.2009.145.pdf) Y, h5 E R$ `7 C
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression .
& Q; a' p/ h9 PNature Protocols 4, 1338–1348 (1 August 2009) | doi:10.1038/nprot.2009.145
' K0 g9 ]- g( c" }2 q（4）http://www.nature.com/nprot/journal/v4/n7/pdf/nprot.2009.95.pdf9 |6 J4 A3 V* b3 Z( e+ e* \3 U+ b9 e) S
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase .
) S" c( ?% W" p$ m2 b* z2 z' @Nature Protocols 4, 1064–1072 (1 June 2009) | doi:10.1038/nprot.2009.95 5 f& V; t0 A; L+ Y7 y4 G+ n5 Z
（5）http://www.nature.com/nprot/journal/v3/n9/pdf/nprot.2008.140.pdf! {; t% C% f0 X. w! E4 }; Y
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification .
. P; I2 n% L8 {5 z; _* _Nature Protocols 3, 1435–1443 (1 August 2008) | doi:10.1038/nprot.2008.140
- B$ f( T4 N& a( x& M$ w（6）http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.67.pdf
% O; V A" I3 J" V$ G g: lImproved northern blot method for enhanced detection of small RNA - ]& X A+ G, W& J4 c
Nature Protocols 3, 1077–1084 (1 June 2008) | doi:10.1038/nprot.2008.67
: {% a* m, h' T（7）http://www.nature.com/nprot/journal/v3/n6/pdf/nprot.2008.73.pdf
5 i C! U: e: d* o* D% jAnalyzing real-time PCR data by the comparative CT method .- K7 {2 f+ \* c0 T) c; w5 q8 ~
Nature Protocols 3, 1101–1108 (1 June 2008) | doi:10.1038/nprot.2008.73
5 h+ l0 | ^, }, D; z( f（8）http://www.nature.com/nprot/journal/v3/n4/pdf/nprot.2008.14.pdf8 N) d9 w( z. b4 r t, f
MicroRNA expression profiling using microarrays .
7 P# |7 y6 `- D0 INature Protocols 3, 563–578 (1 April 2008) | doi:10.1038/nprot.2008.14 |
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发表于 2010-3-1 22:32
