有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章% s6 j7 V9 n8 Y4 ]6 `$ S/ S
2002 Lactate inhibits germ cell apoptosis in the human testis4 {5 Y; H3 C: Z0 ]3 D
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture0 ?+ F0 n; r4 I# g% n( G! Z
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal) J) J& ?$ w! `+ s# b7 ~) d
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells# o( s& p% B+ F; W4 x( R
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro
0 v/ l; v5 m9 r7 }8 N2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture
7 c8 o! U$ K. ~2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
& F: q6 `- j' R/ n3 z2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
% \% F( H* R7 @. I& |. \7 l# j9 K2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse! l) h* `2 S6 |
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines! r9 }+ ]* A  J+ D
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells" q7 g5 [3 [9 O  N6 e. t( f
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells  y' v$ E/ ?, E
2005 Anchorage-independent growth of mouse male germline stem cells in vitro0 h& B5 N7 F3 E: u8 J. J( _
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
: t, `7 t0 }/ _$ n+ Z' h: Y2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
: j- g( w  N7 f( e5 @( u  e2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
1 X- J4 [9 X& y: x- ?2005 Isolation of male germ-line stem cells; influence of GDNF
& r8 [3 \! `. j* z  h* M% h; U/ O2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions8 |' \; P0 ^# K" g. w- Q
2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
! Q7 q2 T9 }4 o/ k' {2006 In vitro propagation of spermatogonial stem cells from KM mice
7 ^+ p* B/ B# x/ @  z6 R# _: z% h, L# j& l( X& v, N2 `
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的