有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章
2 V5 B; _( }& D- D7 x2002 Lactate inhibits germ cell apoptosis in the human testis  x; V  [, H3 @0 N0 j
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture9 h3 \+ e0 b$ F/ [1 ^
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal" ?$ }$ I  n% s$ _9 G
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells0 N1 V8 e+ T  i' }
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro# x6 C# w' ~6 i, J4 K
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture1 v6 F1 K5 j: v  c* h- ]
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis& T8 `) T; M; x5 D2 n" Z
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture8 @* @1 t' u: ~7 U1 U
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse
2 Y  |8 o! V  L# a4 Z5 G2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines4 n% n# F) o4 H/ s; Z, o# w& X0 h
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells& n( b9 y2 I1 ]2 T
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells# `" w" B- g2 q9 p; r: O- l
2005 Anchorage-independent growth of mouse male germline stem cells in vitro
3 U! A8 c& S; i) ~2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor" ^) O4 C0 j+ s( v( Q
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
* V4 `! [/ N0 @5 t( c2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
% R3 N( M% v1 M+ n0 d2005 Isolation of male germ-line stem cells; influence of GDNF
/ M8 g+ }5 n1 g0 Z2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
8 x+ V8 M) _: u1 t2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture' J% g2 D) c8 I% r! F% s
2006 In vitro propagation of spermatogonial stem cells from KM mice
7 B3 m# z2 M$ g: h' v( B* {+ c1 w0 X$ u& @. j' s
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的