有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章1 g/ D3 y6 ?# e3 {# M
2002 Lactate inhibits germ cell apoptosis in the human testis8 }! v0 {: P+ _7 m) i( i
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
9 H5 y4 D$ R) g( M! {" T2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal
6 v3 t7 ]( R9 \3 k) k2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells  l- }5 c5 v. V; E8 S
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro+ E2 I$ O* g) p: W$ S
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture
9 P" G5 x/ f5 i; H) w) D2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
* |6 X% M8 t4 A$ ~/ T2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture0 r! L4 v4 H& \0 J& |* n
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse. _; N! g* \3 x- M3 u) K
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines
& ^) ~5 {! K. u8 C) \1 Y  |2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
2 h1 m8 f, Z. D% b: S; b" m! B2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells* m  u3 Q) @! }9 {( D, {% ~- q
2005 Anchorage-independent growth of mouse male germline stem cells in vitro
: R+ e6 U. a% t8 W2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
: k/ G* b# A2 d9 X" h5 L2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
  e4 X5 L2 p+ C2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment1 X8 Y, I' s1 u( \3 j% z
2005 Isolation of male germ-line stem cells; influence of GDNF
* v( A- a% C; ~2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
) A0 w0 @1 O% j8 @% f2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
5 n) g, A7 s/ y% [( E4 c5 O4 i2006 In vitro propagation of spermatogonial stem cells from KM mice
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8 r9 ]7 J* f% E: g% y  j上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的