请教piggyBac transposons的缺点。xx

dispassion

从原文上看，piggyBac transposons是不整合的可以移除的。可是Junying Yu对它的评价是
( g$ A. L8 T' rA second approach used seamless excision of
0 ^8 |2 {% N, p5 v( \/ r: lpiggyBac transposons to produce vector and transgene-free
1 C. Q: U. N% s+ \/ q0 n7 e6 V) t9 pmouse iPS cells (10). Although a promising approach, vector7 v, i1 }$ k  s8 d0 Q4 v6 L
removal from human iPS cells produced by this method has9 E+ S3 x1 ]7 ^! T
not yet been reported, and removing multiple transposons is- ]  o7 S& t9 Y) ]
labor intensive.
, @+ v; X, b6 N6 e1 p0 e我想请问她说的缺点是从何而来。谢谢