请教piggyBac transposons的缺点。xx

dispassion

从原文上看，piggyBac transposons是不整合的可以移除的。可是Junying Yu对它的评价是/ [" F# ]6 w& Y3 S0 d
A second approach used seamless excision of
3 k: m& Y5 |2 P. h5 QpiggyBac transposons to produce vector and transgene-free* b5 P4 y3 `, X! |
mouse iPS cells (10). Although a promising approach, vector# }4 c$ i/ m+ p( _- @
removal from human iPS cells produced by this method has7 H) r& O" T+ `' s
not yet been reported, and removing multiple transposons is
$ u9 D* Z6 a: Z9 N8 ~0 P: ~labor intensive.$ M9 ?* b5 E7 W- X
我想请问她说的缺点是从何而来。谢谢