请教piggyBac transposons的缺点。xx

dispassion

从原文上看，piggyBac transposons是不整合的可以移除的。可是Junying Yu对它的评价是6 A9 W0 D, F* W% D
A second approach used seamless excision of
) Q# i% C3 h7 B# WpiggyBac transposons to produce vector and transgene-free
3 d$ ]2 F- W& G: c+ Cmouse iPS cells (10). Although a promising approach, vector6 G% o/ X1 E3 ~. U# u0 g
removal from human iPS cells produced by this method has
& Y- Y3 R/ v1 p- y, W4 Onot yet been reported, and removing multiple transposons is
2 \% d# G, d5 J/ s# A" ^labor intensive.2 v7 o5 `) K2 _; z2 ]3 N+ t% `- W
我想请问她说的缺点是从何而来。谢谢