干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

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干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf( {! u: |, n# l  s% Q) I6 V! N
徐赤裔周建庆方建江廉孝芳" ~( f. j, ?/ ~
【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—
7 c3 R9 c8 P8 \; C! M2 q2 NSCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植
: W5 P$ ?% O9 }组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存
4 N" h; J7 c( o/ y. s活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡
) T  l8 ~1 F- R  r4 K细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，/ x9 r% H; u/ j+ U" t
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗( r1 b  x0 v  ?; Y! U0 ^* B2 X
死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上  _7 e6 ?# X4 e: i( _* ?
升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌
; I3 V6 P! T- y* _4 `样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。
% F& w- D0 @+ j2 I* z2 Y【关键词】干细胞移植心肌梗死心力衰竭
! }( G( o( w0 B! f5 C1 [The Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU
7 P4 t: Z/ H/ J3 w* T' |9 N: O4 P$ Giyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—0 }0 O7 p" Y+ a# N7 V
niversity College of Medicine，Hcmgzhou 310009,China
! _/ {& b- z9 W' H+ Z4 c" Y【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—. V) i: G- ~2 B5 R9 c; p& P. p
tion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—
- e* y7 t2 ~3 n7 ~$ Inines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm( @0 q8 H; {6 o
and ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group
' |+ J+ c; ]3 \+ R4 F6 \: sat 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—
8 }, I% Q. R+ \- Eentiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and0 a2 y; P/ S/ @. J0 o& R" x1 b
apoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．
# Y: o; ~7 V1 g0 x+ J, R* \/ ~Smwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——
  C1 l1 e% |; noeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal$ y9 Y; V5 e* O2 P. k) `/ _
index、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—0 T0 i$ o: Z3 z
trol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．
% f5 m( C! W5 b: iConclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible, D# L+ [/ L* _, P- Y
reason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—8 z! K( @/ j! k, _" c, C3 L, B, y
tion collogen deposition in noninfraetion zone．" Q5 u6 E# Z6 u8 P
【Key words] Stem cells Myocardial infarction Heart failure
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月飘东海

我要看看嘿嘿

siwa

很好，参考一下，谢谢

ligangcangxb

可以参考

在路上

1# 海人 [/b谢谢

luohaijian

哥吃的不是面

天空为我蓝

回复 1# 海人
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    谢谢,看看

桂圆儿

谢谢

study

想看看，谢啦