干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

海人

干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf( s; O9 A! S# {% c6 ~4 \: T( @1 s
徐赤裔周建庆方建江廉孝芳
0 O/ }4 V5 e$ J* W- h【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—8 O. ?: A! ]5 M6 p# n9 k
SCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植
' J; T& {: g! M$ o组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存' o" m: H! y9 @! w& M: O! R& Q; _
活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡9 I1 M, e# Q2 M8 e0 ]6 E
细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，) Q, A. n: L+ E" }
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗2 i0 h0 ?- U" ^+ V6 p7 H$ B9 m
死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上
& ~9 J* V+ X% I5 ^8 |升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌
+ m' W) |6 T9 z7 E样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。
2 H+ p9 Y  s: h' o0 B【关键词】干细胞移植心肌梗死心力衰竭
  ^6 A3 q- a" ?! y$ U8 C( i9 o/ mThe Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU
" a# v+ g' b/ Y, jiyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—9 x: X/ r1 }9 K# y! M
niversity College of Medicine，Hcmgzhou 310009,China
. ~% h. B& s. [3 e! L" P4 U【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—- {1 Q! f% C$ v0 Z$ n( ^3 K. A
tion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—
- m  d1 F. J8 anines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm* L% }; M5 A7 N( y! u: {
and ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group
% [8 I( I2 \9 U5 ^! j) [  r9 c4 dat 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—- O6 Y# t. b0 C! ?) f8 Z+ \
entiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and
. m, f7 y- o4 ^9 Kapoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．* Y, ?2 [& d- F6 S1 v2 @
Smwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——
$ K* E7 p8 w; U' ~" r* soeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal
# l. m0 b: C$ K# i: _% f) gindex、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—
2 m! v8 X9 _0 i( l# D! c% P) }trol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．
! Q1 x/ A# X6 c( G. _" C3 TConclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible& @/ g+ l; m5 b! k
reason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—
) A" m3 I* T7 qtion collogen deposition in noninfraetion zone．3 m% I* u+ s4 u) b# A1 Q
【Key words] Stem cells Myocardial infarction Heart failure8 V+ g5 Y: c' _: l
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月飘东海

我要看看嘿嘿

siwa

很好，参考一下，谢谢

ligangcangxb

可以参考

在路上

1# 海人 [/b谢谢

luohaijian

哥吃的不是面

天空为我蓝

回复 1# 海人
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    谢谢,看看

桂圆儿

谢谢

study

想看看，谢啦