骨髓单个核细胞移植对慢性心力衰竭大鼠心肌修复的远期效果

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骨髓单个核细胞移植对慢性心力衰竭大鼠心肌修复的远期效果.pdf- I5 }: A! C6 d% M
黄海怡1 , 黄　佐1 , 胡国梁1 , 侯　健2" t- n1 x. K+ a2 S" j6 G6 \5 Z
(第二军医大学长征医院1. 心血管内科; 2. 血液科, 上海市200003)8 g7 p! i5 P0 s
[关键词] 　病理学与病理生理学; 　干细胞修复心力衰竭大鼠受损心肌; 　骨髓干细胞移植; 　慢性心力衰竭; 　4 H* Q; L, p+ E& m5 K2 c( ^8 v
心肌修复; 　心功能; 　远期效果
* Y& U- X, O& h[摘　要] 　目的　研究骨髓单个核细胞移植对慢性心力衰竭大鼠心肌的修复作用及其远期效果观察。方法　建' O, |/ Q* v4 F+ i' X2 A& N
立慢性心力衰竭大鼠模型。造模后通过心肌直接注射的方法,于移植组移植DAPI 标记过的骨髓单个核细胞;对照3 Z9 M- f0 m& q2 Q" {
组注射同等剂量PBS 溶液;假手术组仅开胸和关胸。并于造模后即刻、移植后4、8 和12 周,分批分组进行免疫组织9 w  N, k- a  c# Y- l+ n) |
化学和透射电镜的检测。结果　心脏标本切片,荧光显微镜检查证实在细胞移植区有细胞核因被标记而呈荧光的4 r; m+ t' g4 X' y# d! _8 y- Q/ [, C: L
新生心肌和血管。免疫组织化学检查发现细胞移植区有类似心肌细胞的细胞团、心肌特异性结蛋白和肌动蛋白,: t' |6 \4 C3 F/ b, |9 v2 e' U
增殖细胞核抗原检测阳性。透射电镜检查可见不成熟的心肌细胞,有细胞超微结构的变化,胞质中有散在肌丝样( U! L9 x$ E5 g
物质,还可见内膜不完整的新生血管,且阳性物质随时间推移而逐渐增多。对照组和假手术组则没有这些改变,并
, ?4 J" g. x% p" p2 Q: m且在不同时间段出现死亡病例。结论　骨髓单个核细胞移植可有效防止慢性心力衰竭大鼠心功能恶化,促进心肌( B) b/ y1 c, Q8 }4 T( f3 g
和血管再生,在移植后长时间内没有出现新的心肌缺血损害、炎症反应和恶性心律失常,持续修复受损心肌,远期
4 M5 P: d/ u8 N' h1 E, Y  O2 f效果明显。8 u$ r" b3 D+ l& Y
[中图分类号] 　R363 [文献标识码] 　A
2 [( E& a- J# D" w7 u# w2 GThe Long2Term Effects of Mononuclear Bone Marrow Cell Transplantation on Repairing4 Z( }; q+ M4 \' {
Necrotic Myocardium in Chronic Heart Failure Rats" D( k' O% G- ^4 j
HUANG Hai2Yi1 , HUANG Zuo1 , HU Guo2Liang1 , and HOU Jian2) T) T" J% l6 u9 J" A
(1. Department of Cardiovasology ; 2. Department of Haematology , Changzheng Hospital , Second Military Medical University , Shanghai 200003 ,
; S8 b' g4 r7 t1 R# KChina)
- z4 |5 p* h9 |. u  R[ KEY WORDS] 　Chronic Heart Failure ; 　Myocaidium Repairing ; 　Heart Function ; 　Long2Term Effects ; 　Cell Transplan2; Z% X3 j( k( K( {( s
tion ; 　Mononuclear Bone Marrow Cell
) B% {$ r& J- C) `5 d6 a+ ?+ t0 [[ABSTRACT] 　　Aim　To investigate the long2term effects of transplanting mononuclear bone marrow stem cell (MBMC) on
  h- E0 X% @- }! f/ Rrepairing necrotic myocardium in chronic heart failure rats. 　　Methods 　First ,to establish chronic heart failure rat animal mod2
0 Z, |5 m& C4 G& M' i+ s; {el. 　MBMC isolated from the rats were directly injected into the myocardium of transplantation group , control group were injected  `$ g9 Q; s8 n* r
PBS , and sham2operated rats were underwent the surgery but not transplantation. 　At 4 th , 8 th and 12 th week after transplanta2
" e; z" g8 g# e& |tion , myocardium were assessed by immunohistochemistry and electron2microscope. 　　Results 　In heart specimens slice , by; u  o( N) f1 C1 o
immunofluorescence2microscope , it was confirmed that there were newborn cardiomyocyte and blood vessels at the site of transplan2
% J4 H" D/ C/ btation which nucleolus were marked with fluorescence. 　Myogenic cell similar with cardiomyocyte ,desmin and actin could be de2
3 N7 |; n. R2 @tected by immunohistochemistry , proliferating cell nuclear antigen(PCNA) detected the proliferated cell. 　Immature cardiomyo22 m, u. `& P( I  @8 a9 C
cyte ,cell ultrastructure changes and newborn blood vessels which endothelium were not complete could also be detected by elec2
$ o  H( K- e  d) j( B. {# gtron2microscope. 　All these matter became more and more with time went on. 　There were no significant discoveries between& J( P+ g5 U$ c) K, p: W0 a" o2 S
control and sham2operation groups , and at different time , there appeared dead cases. 　　Conclusions 　MBMC transplantation4 Q$ j1 L6 f# P4 |
can effectively prevent heart function deteriorated in chronic heart failure , regenerate cardiomyocyte and blood vessels. 　There are0 Z% n7 j1 J) T- Y5 F  l  f
no bad responses such as new ischemia , inflammation and maliganant arrhythmia. 　MBMC can continue to repair necrotic myo23 j7 n( h) W! T/ F
cardium. 　The long2term effect is notable.
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