从胚胎干细胞诱导卵母细胞和精细胞

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Derivation of Oocytes from Mouse Embryonic Stem Cells
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Karin Hu¨bner,1 Guy Fuhrmann,3 Lane K. Christenson,4 James Kehler,1 Rolland Reinbold,1 Rabindranath De La Fuente,2 Jennifer Wood,4 Jerome F. Strauss III,4 Michele Boiani,1 Hans R. Scho¨ler1*9 l' o& k& Z& p0 N, e9 x. g

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! t& _# f8 J7 l; C  v+ {+ v# AContinuation of mammalian species requires the formation and development of the sexually dimorphic germ cells. Cultured embryonic stem cells are generally
$ c9 g5 X; d' M$ \/ Sconsidered pluripotent rather than totipotent because of the failure to detect germline cells under differentiating conditions. Here we show that& o) H- T5 Q$ Y1 f
mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop
4 K1 R6 Y8 ?6 jinto blastocysts. Oogenesis in culture should contribute to various areas, including nuclear transfer and manipulation of the germ line, and advance studies/ d. K% b& W$ C
on fertility treatment and germ and somatic cell interaction and differentiation.