knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
8 T3 x) {$ t( |: Y# z5 M2 dksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清
. q3 r8 W; y' N3 i2 ?! j1 X1 z3 Z% ]$ e9 f9 l* W, ^3 j
具体如下4 g8 D2 \% p2 t8 ]' e3 ]
1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.6 I- G( M/ _0 x9 s4 x  s
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
! c1 N0 \8 ]8 X7 z& o1 F
. d: u3 T$ A2 ^* m4 rKnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe % i0 P! F7 ?1 v3 ~& d/ R

2 A9 o$ ]% S4 ?
* w6 {9 g( X! n( x    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基: [9 O6 H+ m7 E, W, w
ksr是一种成分明确的无血清配方，可直接替代现行方案 ...
1 L* n5 K9 v" E$ Xdaviehe 发表于 2010-5-14 16:24

5 y" y) K- ^2 b7 L

7 `; Q: J6 M! q
/ m7 ~7 c" S3 J7 Q   谢谢解答！

znhy

回复 5# lifescience1
7 Q% R; U# q3 b8 |. x% Y) V1 [# }
! S6 ?% X7 l- }4 G+ u/ D很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確
' ^6 A3 n2 R. b* t- d但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份
; D7 z9 v3 @# ^0 ]% f5 I只提到專利申請中(可能怕使用者自己調配吧）1 B' P+ H* b: }( d, `" y* D
也就是說8 t) ?& l$ `. U* i& @
成份明確，但是不讓你知道....

简简单单

好，学习了