knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
0 X) g$ N: w2 h, U2 Iksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清$ X0 K4 R1 G4 }5 [' y

: n+ G  c( P. e( y% N0 i. B具体如下' s- z- k& \5 u
1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.0 w, F2 a2 B+ D" H2 e! {
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
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0 L+ t3 v& c  d* C6 _" t+ T! cKnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe ' Y3 K: V6 ?2 v' N1 G

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    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基
7 G: K& F6 H8 U" Aksr是一种成分明确的无血清配方，可直接替代现行方案 ...
- Q. s  J3 G7 }0 i" `$ D' a/ Edaviehe 发表于 2010-5-14 16:24

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* V& \  t7 O( z3 y) [3 j5 T9 V   谢谢解答！

znhy

回复 5# lifescience1
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很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確- ]  z" t* T8 r
但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份4 j' _9 Z: ]$ H1 r$ V5 y5 M
只提到專利申請中(可能怕使用者自己調配吧）
3 }/ f% ~7 c1 O- ]也就是說
% t) ]' H  K" H' Z8 C" u成份明確，但是不讓你知道....

简简单单

好，学习了