knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
" p- Z# w5 R* y) Tksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清
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1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.
" }4 k; x  d& f( P  O2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.# V( i2 W+ Z3 {* D0 m) k5 E. r

' i( K8 T) C, H7 r8 r/ y6 M8 \; GKnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe # E, W( \( b% M
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6 C4 m1 T; Q1 m    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基' o" [6 J4 y( L
ksr是一种成分明确的无血清配方，可直接替代现行方案 ...
4 t9 e+ l6 I0 i( P& T: Qdaviehe 发表于 2010-5-14 16:24

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   谢谢解答！

znhy

回复 5# lifescience1 ( I. O  s% K  K; E# |0 [- g
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很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確
: N+ s4 H" ~& n0 S! T# H& c* i+ v/ }5 V  l但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份6 _1 p. {7 H1 E+ @; c  _' D
只提到專利申請中(可能怕使用者自己調配吧）
0 ]) f$ m" D/ q4 h* K也就是說
* c$ s& F' q( M1 E9 l4 V成份明確，但是不讓你知道....

简简单单

好，学习了