knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
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  S( f' H5 ?7 S, O1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.  _6 Y: ?2 O3 k, J0 A
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe
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    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基$ R% c3 Z6 X6 h
ksr是一种成分明确的无血清配方，可直接替代现行方案 ...1 v* w& e6 N4 W3 U% [1 o, X
daviehe 发表于 2010-5-14 16:24

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   谢谢解答！

znhy

回复 5# lifescience1
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很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確9 O6 N! P/ n" X& ]9 X& S/ ^! m
但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份
5 A% K- S( }8 N4 R, C只提到專利申請中(可能怕使用者自己調配吧）
8 s3 ^$ h% g0 D, c  I, x也就是說& u+ V$ D9 `. ?5 g8 C0 U% h0 X' x' ?) k% v. y
成份明確，但是不讓你知道....

简简单单

好，学习了