knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
. `2 P' v6 }" J& L( L% yksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清3 ~, D' Q3 i- Q: x+ r
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具体如下! i8 M. q4 E  G
1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.% x' G" G& ~* D: }( D8 U
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.
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: v2 _$ p, P' H5 O# m5 EKnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe / _4 C3 e7 U& G# M  \
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( w  }6 q# U. M; ~, x3 E" T5 s    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基
4 ?7 P/ g; o+ p  n) J. aksr是一种成分明确的无血清配方，可直接替代现行方案 .../ d4 {& V. U' s$ J
daviehe 发表于 2010-5-14 16:24

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   谢谢解答！

znhy

回复 5# lifescience1 ! J) A% O/ O8 Q+ O. b# J5 q

. n. d( H5 g9 k6 h( d! {8 n很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確
4 f3 E! b  V- n  a3 q) n& |但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份9 b0 [/ g( R; k) J7 M
只提到專利申請中(可能怕使用者自己調配吧）
1 c1 m+ y" |5 y) j  g0 |也就是說7 W7 C0 D; n3 Q% E7 o. u1 J
成份明確，但是不讓你知道....

简简单单

好，学习了