肝细胞与骨髓间质干细胞共同培养时的肝细胞功能

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3 f4 w. _7 |0 K1 l. b黄艳欣, 杜雅菊, 李宝杰( F2 U5 j+ [5 e. V  t* h4 q
黄艳欣, 杜雅菊, 李宝杰, 哈尔滨医科大学附属第二医院消化内科 黑
  G3 \$ v$ O+ D* H4 T* ~龙江省哈尔滨市 150086+ ^/ M8 H+ U9 \4 c& n* u% o
黄艳欣, 女, 1977-11-14 生, 黑龙江省哈尔滨人, 汉族. 2001 年哈尔滨医科5 Y6 M$ X6 r! Y% k" J
大学硕士生.1 J( P, M) a( v: |, h$ E
项目负责人: 杜雅菊, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学附属第
1 Z1 \7 U6 v1 I6 L二医院消化内科.
( S0 J- G. T. O5 @8 [" X电话: 0451-86605143
7 O2 ]3 C% U! E! m( {( p收稿日期: 2003-12-10 接受日期: 2004-02-01# l4 t* W+ g, A1 h
Effect on function of rat hepatocytes
* q6 Z% l. V7 P9 a% U1 f6 p( x+ `& tcultured with bone marrow cells# Q8 F8 f( O( T. z0 y1 ^
Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li
* Q% |# u9 ^! \# u6 E  VYan-Xin Huang, Ya-Ju Du, Bao-Jie Li, Department of Gastroenterology,
% @  W( H+ l2 v3 R1 |* pThe Second Hospital of Harbin Medical University, Harbin 150086,
  t9 U" ~. o+ E; ~$ @. ?2 e0 _' ~6 zHeilongjiang Province, China
1 `$ E4 X! F8 n  H0 E$ }+ E  H- ^8 FCorrespondence to: Dr. Ya-Ju Du, Department of Gastroenterology,* d0 @# H- P3 T% W" p) l1 X0 c' J
The Second Hospital of Harbin Medical University, Harbin 150086,
3 J6 O9 ]1 h9 n5 p8 wHeilongjiang Province, China
, J7 y! I" R1 ^( GReceived: 2003-12-10 Accepted: 2004-02-014 p2 d0 L0 O8 w& i+ b( t  _
Abstract
/ k& |4 n, E! [" xAIM: To study of the effect on the function of rat hepatocytes
* ?- h( f8 O% lcultured with bone marrow cells.
( f: _0 ?6 F4 n2 g+ P* D+ ~* nMETHODS: Rat hepatocytes were isolated by the modified
# o) q+ o* ]* C# A$ A0 Ltwo-step method described by Seglen. The primary cultured! V* O: R3 |1 T/ i
hepatocytes and bone marrow cells were served as cocultured
4 H& A3 F+ r- J; b+ G: A# F' Vgroup, and single cultured hepatocytes as control group. The
) F/ a' ~+ C( I0 `0 c& B0 Oyield and viability were assessed by trypan blue exclusion.
+ x! F: v# I# s  s/ c) r& x9 s' ]The morphologic changes of cultured hepatocytes were& \) Y' v* y6 g( n3 D. F
observed. The concentrations of albumin and urea in the" m+ e# W# E3 ~: `; s9 p
supernatant in different cultural period were examined.
- p7 s- w2 }: r) G- M7 K, WRESULTS: The albumin synthesis (13.75>2.179, P <0.05)
; t7 p& c0 }. Oand urea level (7.27>2.179, P <0.05) had fluctuating
% N- c$ `/ c5 Z$ j7 Dchanges in one week, and cocultured group had higher9 l1 o# Y! T/ R1 Z! j, f! Y
albumin synthesis and urea level.
4 ]8 I0 ~/ T4 RCONCLUSION: Cocultured hepatocytes with bone marrow
" @8 n1 T$ Y2 b* Wcells can improve the function of hepatocytes.
3 o7 B& k) l5 c6 j' Q' \Huang YX, Du YJ, Li BJ. Effect on function of rat hepatocytes cultured with
2 N: ^/ H& N/ n/ wbone marrow cells. Shijie Huaren Xiaohua Zazhi 2004;12(5):1129-1131* `4 f' @, C! M8 [& K( S0 h8 u
摘要
1 V3 @2 @4 S, k3 \! l, |$ a$ [$ d目的: 利用原代培养的大鼠肝细胞与骨髓间质干细胞共同培
5 R( f8 S2 _/ p' @" Q7 U) a1 R养研究骨髓间质干细胞对肝细胞功能的影响以便更好
' h* L+ d! i% d4 t, E用于肝细胞移植及生物人工肝.. {3 g( ?# N* M! O; ?
方法: 采用低浓度胶原酶原位循环灌流法分离大鼠肝细胞
( h7 E% `  F! c. p3 o. ?9 Q, N获得有活性的肝细胞进行原代培养. 台盼蓝排斥法计算细胞1 J5 f! l* d+ @- I' e4 P  e" u8 L
产量和细胞活性; 光镜下动态观察细胞形态学改变并收集不同时期培养上清检测其细胞分泌等功能. 比较肝细胞单
  ?- ]6 F) A* o8 X' y4 M纯培养及其与骨髓间质干细胞共同培养时细胞的功能., ?/ C% _3 H7 K2 `& r) t8 [2 `" C
结果: 培养7 d时两组均仍然维持白蛋白分泌及尿素合成功
: g$ c) t. i3 b能; 共同培养组与单纯肝细胞培养组在白蛋白分泌 (13.75># ?, Z, H1 I/ O2 h: _1 W
2.179, P <0.05) 及尿素合成功能 (7.27>2.179, P <0.05) 存在
+ o+ ~1 N: t# W显著性差异共同培养组明显高于单纯培养组.
: s0 T" @( @8 a5 m: v3 M结论: 肝细胞与骨髓间质干细胞共同培养可以使肝细胞
) e9 `7 y) J' z( ]' T维持特异性功能提高肝细胞活性.) y! M! d/ T4 e* u9 @, ]

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feng123456

想要学习一下

missyoudad

好文章

yuheng

MSC分泌因子支持肝细胞生长，可是比较怀疑单纯共培养是否能有那么好的效果？

tjutiger

baiduyixia

cncpht

good

yubingzheng

谢谢楼主

碧海蓝天

学习学习

baichunyu

1# cdk1 ) P. h# v# o; ^
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4 g: M. x$ m0 y+ D! a支持