肝细胞与骨髓间质干细胞共同培养时的肝细胞功能

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9 R0 \+ }0 z# O$ z$ B* g  q黄艳欣, 杜雅菊, 李宝杰
2 _/ P: _( n( ]黄艳欣, 杜雅菊, 李宝杰, 哈尔滨医科大学附属第二医院消化内科 黑
6 h6 r) {2 k+ i9 n- O" @0 _龙江省哈尔滨市 150086
' }+ }7 b; ~% X  I$ u( X5 @6 a黄艳欣, 女, 1977-11-14 生, 黑龙江省哈尔滨人, 汉族. 2001 年哈尔滨医科+ h+ m$ {2 d, ?: T  g* y! [1 O
大学硕士生./ b) K) o3 I/ p* }/ c% d2 [* R
项目负责人: 杜雅菊, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学附属第
# c: A9 [4 p% S4 n& l* i2 Q二医院消化内科.8 }2 P" a% W2 ^/ w2 D2 h" ~
电话: 0451-86605143
5 w3 i- E) N# L$ k收稿日期: 2003-12-10 接受日期: 2004-02-01
& @* X- g; w7 A2 U. MEffect on function of rat hepatocytes0 _. O2 e9 {1 L* q
cultured with bone marrow cells5 ~& n, G1 y. {& C( z/ }. D
Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li
4 y: A# x) ?  b4 ]Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li, Department of Gastroenterology,: }) ]1 z: j5 c
The Second Hospital of Harbin Medical University, Harbin 150086,
$ E1 F( i  X, f  ^7 |Heilongjiang Province, China( G+ w! v. k, p3 j
Correspondence to: Dr. Ya-Ju Du, Department of Gastroenterology,# n  \1 z* O; V' B+ z
The Second Hospital of Harbin Medical University, Harbin 150086,- E* Q8 Q$ [3 B2 B1 M  }
Heilongjiang Province, China
! J* f! N% S# W$ x# i% yReceived: 2003-12-10 Accepted: 2004-02-01
, ~7 |+ D8 y: jAbstract% }$ y* a" w+ O9 w. e6 a; P
AIM: To study of the effect on the function of rat hepatocytes
  @( O3 J4 q: s5 x- ~cultured with bone marrow cells.
0 F- c5 P3 T$ M% B# J$ y9 ?# yMETHODS: Rat hepatocytes were isolated by the modified) S+ j  Z# F6 ~) |0 y
two-step method described by Seglen. The primary cultured9 T/ l$ E" {! ]+ R) L; ^
hepatocytes and bone marrow cells were served as cocultured0 F# u% l% A) Q7 }1 u  b
group, and single cultured hepatocytes as control group. The. Y' E$ @# i& ]9 J" p* K
yield and viability were assessed by trypan blue exclusion.
  W% F* l! A) l, Z5 h6 gThe morphologic changes of cultured hepatocytes were3 T2 P5 t1 w0 H; N% ]
observed. The concentrations of albumin and urea in the
6 }# c3 m# u; P+ y9 u5 E, Xsupernatant in different cultural period were examined.3 L% R1 O8 g+ K/ R. {- B# \
RESULTS: The albumin synthesis (13.75>2.179, P <0.05)) w! a3 f% x$ J5 u
and urea level (7.27>2.179, P <0.05) had fluctuating
( H2 a6 w/ G% G& y6 U7 Ichanges in one week, and cocultured group had higher. F2 Y, H$ c" y
albumin synthesis and urea level.
' c7 Z& o( ~+ Q& u0 B9 VCONCLUSION: Cocultured hepatocytes with bone marrow
, p5 _$ w0 C. q9 Rcells can improve the function of hepatocytes.
- T; @* Y/ [* b" t5 U4 }Huang YX, Du YJ, Li BJ. Effect on function of rat hepatocytes cultured with
5 @2 F( y/ d& pbone marrow cells. Shijie Huaren Xiaohua Zazhi 2004;12(5):1129-11314 P' E: F( y% ^1 I
摘要
  B9 \+ c% m) I; U4 |; w5 o目的: 利用原代培养的大鼠肝细胞与骨髓间质干细胞共同培7 r: w% z/ u& R
养研究骨髓间质干细胞对肝细胞功能的影响以便更好
% ~' e/ w  U  a" `) i( r6 r用于肝细胞移植及生物人工肝.+ L6 I: d) l0 y+ h" q
方法: 采用低浓度胶原酶原位循环灌流法分离大鼠肝细胞% P$ r8 ?: y8 _. a
获得有活性的肝细胞进行原代培养. 台盼蓝排斥法计算细胞
( u" @' E5 b9 ~% m( {$ g  z! _5 l' V产量和细胞活性; 光镜下动态观察细胞形态学改变并收集不同时期培养上清检测其细胞分泌等功能. 比较肝细胞单
4 y$ E& E6 f5 [8 e7 h# r6 ], j纯培养及其与骨髓间质干细胞共同培养时细胞的功能.
9 ?# V# R" D: i( R( X: z' \  c结果: 培养7 d时两组均仍然维持白蛋白分泌及尿素合成功
/ L7 l9 d% ^$ a: k9 i& ^! G' p/ C# G能; 共同培养组与单纯肝细胞培养组在白蛋白分泌 (13.75>
: G+ P: s2 g/ e. Z2.179, P <0.05) 及尿素合成功能 (7.27>2.179, P <0.05) 存在2 e4 a9 e- \3 ?" ~. D8 @$ \* T3 O
显著性差异共同培养组明显高于单纯培养组.
% l% \4 m3 m4 F! s+ e结论: 肝细胞与骨髓间质干细胞共同培养可以使肝细胞- U: G+ h4 B9 ?# ^+ k+ x
维持特异性功能提高肝细胞活性.
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