外周血干细胞与组织工程骨移植修复犬牙周骨缺损区转化生长因子-β的表达

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# r9 z0 V8 t8 g4 N# R赵淑贤,赵东方,王　敏,张建国,董　凯,张　兰
" Y/ M* t5 D: k- \! B! ^) H2 h[摘要] 　目的　探讨外周血干细胞(APBSC) 与组织工程骨(TEB) 复合移植修复牙周骨缺损中转化生长因子(TGF) -β的表达。
8 J" l3 q" a5 y5 M3 f( n方法　犬的牙周骨缺损区移植APBSC - TEB 后,用免疫组织化学方法观察不同时期APBSC 分化、增殖成为成骨细胞过程中
1 L5 B) ~4 P# ^) c4 ^- \TGF -β的表达,观察TGF -β诱导APBSC 分化、增殖成骨细胞的情况。结果　实验组在APBSC - TEB 移植2 周,即见骨缺损区% O4 i& |9 L4 }6 o/ h# f
边缘TGF -β呈弱阳性表达,4～8 周骨缺损区中心区域可见大量的成骨细胞、成纤维细胞和胶原纤维呈现强阳性表达,12 周骨! t2 r$ s) w6 D9 U$ B6 K: t; o  a! U3 ^
缺损区已完全修复。而对照组TEB 移植8～12 周,仅在骨缺损边缘区域TGF -β呈弱阳性反应。结论　APBSC 在骨缺损区分
: S9 q, P% n) z( @5 P. k0 T) o化、增殖为成骨细胞的过程中TGF -β是重要的调节因子,具有诱导成骨的作用。- z5 q. ~3 Z( h, ]$ }0 Z3 s
[关键词] 　外周血干细胞; 免疫组织化学; TGF -β表达; 犬
* K8 @" I% K) U! `  F# x6 B[中图分类号] 　R781. 4 　　[文献标识码] 　A 　　[文章编号] 　100329872 (2007) 0520236203& F+ {6 _& D5 R5 L9 m! X! M
Expression of TGF2β in region of dog experimental alveolar bone defect repaired by Transplantation APBSC2TEB1 y, o2 u' O9 ]) n/ g- r8 k" s
ZHAO Shu2xian , ZHAO Dong2fang , WANG Min , ZHANG Jian2guo , DONG Kai , ZHANG Lan. ( Department of Oral Medicine , Stomatological
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" _9 J5 I( Z3 @; W: W' j$ L9 vAbstract : 　Objective 　To study the positive expression of TGF2βin the region of dog experimental alveolar bone defect repaired by Trans2
) A  Y" p8 G3 i: m8 p% m- fplantation APBSC2TEB. Methods 　After the APBSC2TEB transplantation was introduced into the dog alveolar bone defect region ,the positive
; I! J! L: j: hexpression of TGF2βof APBSC proliferation and differentiation into osteoblast was observed. Results 　After 2 weeks ,a weak2positive expression6 P8 c" Y; H. @: l7 O
of TGF2B in the purlieus region of dog experimental alveolar bone defect was found. After 428 weeks ,the osteoblast ,collagenous fiber and fi2
5 {+ d, R0 M+ b. @- V7 Dbroblast showed strong2positive expression of TGF2βin the centre region of alveola bone defect in the experimental group. The alveolar bone de2
" D4 P5 u! n: T9 w7 K; O. K. Xfect was repaired on the 12th week and only weak2positive expression of TGF2βin the purlieus region of alveolar bone defect was found in the
# v# M4 H9 i' mcontrol group. Conclusion 　TGF2βis an important factor that promotes proliferation and differentiation of APBSC into osteoblast in the alveolar
. G+ D/ v% W/ Xbone defect. TGF2βis effective on bone growth.
* l, Z) Y4 d* `, k, o( ]" R* C, ?, [4 ZKey words : 　autologous periphera blood stem cell ; immunity tissue chemistry ; expression of TGF2β; dogs
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绮梦

谢谢

luwei8157

该文献中，& K" [' ]. F8 G  ?
对外周血干细胞在移植区如何示踪？
+ c$ B6 L' I2 e/ k& i  i- z移植区TGF-b表达是机体对细胞的反应，还是细胞自身分化的表现？

巭秂

谢谢

dongxin

谢谢分享
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