诱导人脐血神经干细胞向神经细胞分化的研究

cdk1

6 _) N. {7 g% S. Z

8 S) d3 ^# b8 d) o" e2 f& g: u季旭东1 , 高　超2 , 杨月景3 , 刘计荣40 K4 B0 [3 s( E
(11 郑州大学口腔医学院口腔内科　河南郑州　450052 ;21 郑州市儿童医院神经内科　河南郑州　450053 ;
6 b! c" z* P7 G7 u+ F$ c31 郑州大学第二附属医院消化内科　河南郑州　450003 ;41 郑州大学基础医学院　河南郑州　450052)
* m# \9 [* z5 y. p$ @9 E) L& B摘要: 目的: 体外诱导人脐血单个核细胞(mononuclear cells ,MNCs) 中的神经干细胞(neural stem cells ,NSCs) 向
+ m3 X2 y2 M" r; f- h* _神经细胞分化的研究。方法: 体外培养中用表皮生长因子(epithelium growth factor ,EGF) 和碱性成纤维细胞# e5 p& {4 q! y3 W' B
生长因子(basic fibroblast growth factor ,bFGF) 诱导人脐血NSCs 向神经细胞的分化。结果: 在EGF 和bFGF 作0 X, F# k& M; K9 G
用下,实验组Nestin 阳性细胞数量显著增多;在EGF 和bFGF 作用下,Nestin 的mRNA 表达PCR 条带变量两组
6 e3 K! h* N0 o8 g间有显著性差异。结论: 细胞因子EGF 和bFGF 在体外能促进脐血MNCs 中NSCs 的分化,联合应用较单独
$ G" f" h- Z. r! ^. i% T应用具有更强的促进脐血MNCs 向神经样细胞分化作用。
* ?* t+ j3 `. ]! s  X* u7 I关键词: 脐血; 神经干细胞; EGF; bFGF: s5 m0 _& V# x4 T" R- ?: q5 a+ W( M
中图分类号: R32912 　　文献标识码: A
! f( I; `/ E  |3 ~8 tInvestigation of differentiation derived from human umbilical
$ B1 B1 {$ P1 h9 G8 acord blood into neuron-like cells7 I! X+ j  ~/ `1 k
J I Xu-dong1 , GAO Chao2 , YANG Yue-jing3 , LIU Ji- rong4
$ U% _' A) U9 C1 R6 t4 N! j$ s( 1. Department of Oral Medicine , The College of Stomatology of Zhengzhou University , Zhengzhou 450052 , China ; 2. Department of Nerve
$ z2 {' U# b0 I: r% ?. DMedicine Chidren’s Hospital of Zhengzhou , Zhengzhou 450053 , China ; 3. Department of Digestion , The Secend Affiliated Hospital of
- Y1 g+ u4 g  r' E) j, F, m( OZhengzhou University , Zhengzhou 450052 , China ; 4. Department of Pathophysiology , Basic Medical School of Zhengzhou University ,
6 t8 m1 P. ]+ W8 a( F  WZhengzhou 450052 , China)9 B2 t# B" E* R0 g
Abstract : Objective : To investigate differentiation of nerve stem cells in mononuclear cells derived
* u' i- P% \7 E% j( \from human umbilical cord blood into neuron-like cells in vitro. Methods : To induce the differentiation+ ^$ w$ g; h2 g4 a7 s" t
of nerve stem cells into neuron-like cells with epithelium growth factor ( EGF) and basic fibroblast
. Y+ P3 t' o& @# t8 ngrowth factor (bFGF) . Results : In the role of EGF and bFGF ,the nember of nestin’s positive cell is
- ^) ^! w6 M: C# Nsignificant increasing in the experimental group. The belt of PCR which is expressed by nestin’s mRNA
7 y" c' ^( K; B# Kis more brighter in experimental group ,there are significant difference in two groups. Conclusion : EGF
$ y8 f3 u, e0 ]4 x5 v# \: rassociated with bFGF can improve the differentiation of nerve stem cells into neuron-like cells.
3 e$ f, a* \/ k- `% `* K1 sKeywords : mononuclear ; NSCs ; EGF ; bFGF  b3 n$ J( V4 X/ s

4 S* A  X+ k0 u% a( p附件是pdf全文。感谢大家支持，回复本贴就可以下载了
, W8 k; S6 B# P8 t( q+ W8 Q
. `2 [) y: i4 L5 c

gaoling

回复

linfeifei

谢谢，感谢分享

蓝猫3000

不得不谢

dy183

非常感谢

cz200203

thanks

雅孜

我来学习啦